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Scholarly Interest Report
         
James A. McNew
Professor
Professor in Biochemistry and Cell Biology
 
e-mail:mcnew@rice.edu
 
  • B.S. Biochemistry (1989) Texas A&M University, College Station, Texas
  • Ph.D. Pharmacology (1994) University of Texas Southwestern Medical Center, Dallas, Texas
  • Postdoctoral Training Cell Biology (1995-2000) Memorial Sloan-Kettering Cancer Center, New York, New York
 
Primary Department
   Department of BioSciences
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Department Affiliations
 
  • Institute of Biosciences and Bioengineering
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    Websites
     McNew Lab
     
    Research Areas
     Molecular mechanism of biological membrane fusion. Peroxisome biogenesis. membrane protein expression and reconstitution, intracellular vesicular transport, ER homotypic fusion
     

    Research Statement 2010

     

    My lab is interested in the molecular mechanisms of membrane fusion. Membrane fusion is a fundamental process involved in diverse cellular events such as fertilization and neuroscretion. Biological membrane fusion relies on proteins to drive membrane merger. mcnew_workA family of integral membrane proteins collectively known as SNAREs mediates the fusion of intracellular transport vesicles. While SNAREs pair in specific ways to provide the mechanical energy to drive fusion, the delicate interplay of regulatory elements that orchestrate this event in space and time remain elusive. We use biochemistry, cell biology and molecular genetic techniques to study the assembly and regulation of SNARE proteins in two model systems: vesicular transport in the budding yeast S. cerevisiae and neurosecretion in the fruity D. melanogaster. We have initially focused our attention on the regulatory role of Sec1p in yeast exocytosis. We have recently shown that Sec1p binds primarily to the yeast t-SNARE complex and directly stimulates membrane fusion. We are currently examining the precise method of Sec1p stimulation as well as analyzing the function of the Sec1p family member ROP in neuronal exocytosis. Additional studies in the lab involve membrane fusion events in other aspects of yeast cell biology including sporulation and cell-cell fusion during mating.

     
    Selected Publications
     Abstracts
     

    Faust, J.E., Manisundaram, A., Stern, M., and McNew, J.A. "Peroxisome Biogenesis in Drosophila melanogaster."  (March 7-11, 2012)

     
     

    Chu, P., Faust, J., Stern, M., and McNew, J.A. "Stressed and hungry flies: The effects of peroxisome loss on oxidative stress and lipid metabolism."  (April 2010)

     
     

    Dos Santos, E.D., Moss, T.J., McNew, J.A. "Atlastin mediated membrane fusion."  (April 2010)

     
     

    Manisundaram, A., Faust, J., Stern, M., and McNew, J.A. "Generation of a Knockout Of Pex3 Gene In Drosophila Melanogaster."  (April 2010)

     
     

    Moss, T.J., and McNew, J.A. "Molecular analysis of homotypic membrane fusion by the GTPase atlasin, Biochemistry of Membrane Traffic: Secretory and Endocytic Pathways."  (October 2010)

     
     

    Moss, T.J., and McNew, J.A. "Molecular analysis of membrane fusion by Drosophila atlastin."  (December 2010)

     
     

    Peng, C., Moss, T., and McNew, J.A. "Improving Expression and Purification of Atlastin and Marf Proteins."  (April 2010)

     
     

    Doneske, B., Schaub, J. R., Lu, X., Shin, Y-K, and McNew, J. A. "Hemifusion Arrest by Complexin Is Relieved by Ca2+-Synaptotagmin I." American Society for Cell Biology (December 9, 2006)

     
     

    Rodkey, T.L., and McNew, J.A. "Neuronal Sec1 Binds the t-SNARE Complex and Stimulates Fusion." American Society for Cell Biology (December 9, 2006)

     
     

    Schaub, J.R., Liao, J., and McNew, J.A. "Examination of Complexin Function During SNARE-mediated Fusion in vitro." Rice Undergraduate Research Symposium (April 21, 2006)

     
     

    Stoops, E., and McNew, J.A. "Disassembly of a Prototypical Ternary Complex: Examining the Role of Sec9 in SNARE Complex Recycling." Rice Undergraduate Research Symposium (April 21, 2006)

     
     Refereed articles
     

    Chen S, Desai T, McNew JA, Gerard P, Novick PJ, Ferro-Novick S "Lunapark stabilizes nascent three-way junctions in the endoplasmic reticulum." Proc Natl Acad Sci U S A, 112(2) (2015) : 418-23.


    doi: 10.1073/pnas.1423026112.
     
     

    Faust JE, Desai T, Verma A, Ulengin I, Sun TL, Moss TJ, Betancourt-Solis MA, Huang HW, Lee T, McNew JA "The Atlastin C-terminal Tail Is an Amphipathic Helix That Perturbs the Bilayer Structure during Endoplasmic Reticulum Homotypic Fusion." J Biol Chem., 290(8) (2015) : 4772-83.


    doi: 10.1074/jbc.M114.601823
     
     

    Faust JE, Manisundaram A, Ivanova PT, Milne SB, Summerville JB, Brown HA, Wangler M, Stern M, McNew JA. "Peroxisomes are required for lipid metabolism and muscle function in Drosophila melanogaster." PLoS One, 9(6) (2014) : e100213.


    doi: 10.1371/journal.pone.0100213
     
     

    McNew JA, Sondermann H, Lee T, Stern M, Brandizzi F. "GTP-dependent membrane fusion." Annu Rev Cell Dev Biol, 29 (2013) : 529-550.

     
     

    Shen D, Yuan H, Hutagalung A, Verma A, Kümmel D, Wu X, Reinisch K, McNew JA, Novick P. "The synaptobrevin homologue Snc2p recruits the exocyst to secretory vesicles by binding to Sec6p." J Cell Biol, 202(3) (2013) : 509-26.

     
     

    Faust, J.E., Peng, C., and McNew, J.A. "An inventory of peroxisomal proteins and pathways in Drosophila melanogaster." Traffic (2012)

     
     

    Kim, K., Petrova, Y.M., Scott, B.L., Nigam, R., Agrawal, A., Evans, C.M., Azzegagh, Z., Gomez, A., Rodarte, E.M., Olkkonen, V., Bagirzadeh, R., Piccotti, L., Ren, B., Yoon, J-H., McNew, J.A., Adachi, R., Tuvim, M., Dickey, B.F. "Munc18b Is an Essential Gene in Mice Whose Expression Is Limiting for Secretion by Airway Epithelial and Mast Cells." Biochem J. , 446 (2012) : 383-94.

     
     

    Stefano, G., Renna, L., Moss, T.J., McNew, J.A., and Brandizzi, F. "In Arabidopsis the spatial and dynamic organization of the Endoplasmic Reticulum and Golgi apparatus is influenced by the integrity of he C-terminal domain of RHD3, a non-essential GTPase." Plant J., 69 (2012) : 957-966.

     
     

    Moss, T.J, Daga, A., McNew, J.A. "Fusing a lasting relationship between ER tubules." Trends in Cell Biology, 21 (2011) : 416-23.

     
     

    Moss, T.J., Andreazza, C., Verma, A., Daga, A. and McNew, J.A. "Membrane fusion by the GTPase atlastin requires a conserved C-terminal cytoplasmic tail and dimerization through the middle domain." Proc Natl Acad Sci USA, 108 (2011) : 11133-38 .

     
     

    Nair, U., A. Jotwani, J. Geng, N. Gammoh, D. Richerson, W.L. Yen, J. Griffith, S. Nag, K. Wang, T. Moss, M. Baba, J.A. McNew, X. Jiang, F. Reggiori, T.J. Melia, and D.J. Klionsky "SNARE proteins are required for macroautophagy." Cell, 146 (2011) : 290-302.

     
     

    Pendin, D., McNew, J.A. and Daga, A. "Balancing ER dynamics: shaping, bending, severing, and mending membranes." Curr Opin Cell Biol (2011)

     
     

    Pendin, D., Tosetto, J., Moss, T.J., Andreazza, C., Moro, S., McNew, J.A., and Daga, A. "GTP-dependant packing of a 3-helix bundle is required for atlastin mediated fusion." Natl Acad Sci USA, 108 (2011) : 16283-88 .

     
     

    Orso, G., Pendin, D., Liu, S., Tosetto, J., Moss, T.J., Faust, J.E., Micaroni, M., Polishchuk, R.S., Martinuzzi, A., McNew, J.A., and Daga, A. "The dynamin-like GTPase atlastin is required for homotypic fusion of endoplasmic reticulum membranes." Nature, 460 (2009) : 978-983.

     
     

    Brandie, F.M., Aran, V., Verma, A., McNew, J.A., Bryant, N.J., and Gould, G.W. "Negative regulation of syntaxin4/SNAP-23/VAMP2-mediated membrane fusion by Munc18c in vitro." PLoS One, 3 (12) (2008) : e4074.

     
     

    Curtis, L.B., Doneske, B., Liu, X., Thaller, C., McNew, J.A., and Janz, R. "Syntaxin 3B is a t-SNARE specific for ribbon synapses of the retina." J. Comp. Neurol., 510 (5) (2008) : 550-559.

     
     

    McNew, J.A. "Regulation of SNARE-mediated membrane fusion during exocytosis." Chem. Rev., 108 (5) (2008) : 1669-1686.

     
     

    Rodkey, T.L., Liu, S., Barry, M., and McNew, J.A. "Munc18a scaffolds SNARE assembly to promote membrane fusion." Molecular Biology of the Cell, 19 (12) (2008) : 5422-5434.

     
     

    Yang, H.J., Nakanishi, H., Liu, S., McNew, J.A., and Neiman, A.M. "Binding interactions control SNARE specificity in vivo." J. Cell. Biol., 183 (6) (2008) : 1089-1100.

     
     

    Liu, S., Wilson, K.A., Rice-Stitt, T., Neiman, A.N., and McNew, J.A. "In vitro fusion by the sporulation specific t-SNARE light chain Spo20p is stimulated by phosphatidic acid." Traffic, 8 (2007) : 1630-1643.

     
     McNew, J.A. (2008). Regulation of SNARE-Mediated Membrane Fusion during Exocytosis. Chem Rev.
     
     

    Schaub, J.R., Lu,X., Doneske, B., Shin, Y-K., and McNew, J.A. "Hemifusion arrest by complexin is relieved by Ca2+-Synaptotagmin I." Nature Structural & Molecular Biology, 13 (2006) : 748-750.

     
     

    Van Komen, J.S., Bai, X., Scott, B.L., and McNew, J.A. "An intramolecular t-SNARE complex functions in vivo without the syntaxin N-terminal regulatory domain." J. Cell Biol., 172 (2006) : 295-307.

     
     

    Lu, C., Zhang, F., McNew, J.A., and Shin, Y.K. "Membrane fusion induced by neuronal SNAREs transits through hemifusion." J. Biol. Chem., 280 (2005) : 30538-30541.

     
     

    Van Komen, J.S., Bai, X., Rodkey, T.L., Schaub, J., and McNew, J.A. "The polybasic juxtamembrane region of Sso1p is required for SNARE function in vivo." Eukaryotic Cell, 4 (2005) : 2017-2028.

     
     

    Xu, Y., Zhang, F., Su, Z., McNew, J.A., and Shin, Y.K. "Hemifusion in SNARE-mediated membrane fusion." Nat. Struct. Mol. Biol., 12 (2005) : 417-422.

     
     

    McCartney, A.W., Dyer, J.M., Dhanoa, P.K., Kim, P.K., Andrews, D.W., McNew, J.A., and Mullen, R.T. "Membrane-bound fatty acid desaturases are inserted co-translationally into the ER and contain different ER retrieval motifs at their carboxy termini." Plant J., 37 (2004) : 156-173.

     
     

    Scott, B.L., Van Komen, J.S., Irshad, H., Liu, S., Wilson, K.A., and McNew, J.A. "Sec1p directly stimulates SNARE-mediated membrane fusion in vitro." J. Cell Biol., 167 (2004) : 75-85.

     
     

    Yan, Q., Sun, W., McNew, J.A., Vida, T.A., and Bean, A.J. "Ca2+ and N-ethylmaleimide-sensitive factor differentially regulate disassembly of SNARE complexes on early endosomes." J. Biol. Chem., 279 (2004) : 18270-18276.

     
     

    Melia, T.J., Weber, T., McNew, J.A., Sollner, T.H., and Rothman, J.E. "Regulation of membrane fusion by the membrane-proximal coil of the t-SNARE during zippering of SNAREpins." J. Cell Biol., 158 (2002) : 929-940.

     
     

    Parlati, F., Varlamov, O., Paz, K., McNew, J.A., Hurtado, D., Sollner, T.H., and Rothman, J.E. "Distinct SNARE complexes mediating membrane fusion in Golgi transport based on combinatorial specificity." Proc. Natl. Acad. Sci. U.S.A., 99 (2002) : 5424-5429.

     
     

    Paumet, F., Brugger, B., Parlati, F., McNew, J.A., Sollner, T., and Rothman, J.E. "A t-SNARE of the endocytic pathway must be activated for fusion." J. Cell Biol., 155 (2001) : 961-968.

     
     

    Brugger, B., Nickel, W., Weber, T., Parlati, F., McNew, J.A., Rothman, J.E., and Sollner, T.H. "Putative fusogenic activity of NSF is restricted to a lipid mixture whose coalescence is also triggered by other factors." EMBO J., 19 (2000) : 1272-1278.

     
     

    Fukuda, R., McNew, J.A., Weber, T., Parlati, F., Engel, T., Nickel, W., Rothman, J.E., and Sollner, T.H. "Functional architecture of an intracellular t-SNARE." Nature, 407 (2000) : 198-202.

     
     

    McNew, J.A., Parlati, F., Fukuda, R., Johnston, R.J., Paz, K., Paumet, F., Sollner, T.H., and Rothman, J.E. "Compartmental specificity of cellular membrane fusion encoded in SNARE proteins." Nature, 407 (2000) : 153-159.

     
     

    McNew, J.A., Weber., T., Parlati, F., Johnston, R.J., Melia, T.J., Sollner, T.H., and Rothman, J.E. "Close is not enough: SNARE-dependent membrane fusion requires an active mechanism that transduces force to membrane anchors." Journal of Cell Biology, 150 (2000) : 105-117.

     
     

    Parlati, F., McNew, J.A., Fukuda, R., Miller, R., Sollner, T.H., and Rothman, J.E. "Topological restriction of SNARE-dependent membrane fusion." Nature, 407 (2000) : 194-198.

     
     

    Weber, T., Parlati, F., McNew, J.A., Johnston, R.J., Westermann, B., Sollner, T.H., and Rothman, J.E. "SNAREpins are functionally resistant to disruption by NSF and alphaSNAP." J. Cell Biol., 149 (2000) : 1063-1072.

     
     

    Lupashin, V.V., Pokravskaya, I.D., McNew, J.A., and Waters, M.G. "Characterization of a novel yeast SNARE protein implicated in Golgi retrograde traffic." Molec. Biol. Cell, 8 (1997) : 2659-2676.

     
     

    McNew, J.A., Coe, J.G.S., Sogaard, M., Zemelman, B.V., Wimmer, C., Hong, W., and Sollner, T.H. "Gos1p, a Saccharomyces cerevisiae SNARE protein involved in Golgi transport." FEBS Lett., 435 (1998) : 89-95.

     
     

    McNew, J.A., Hartmann, E., and Sollner, T.H. "Vesicular flow patterns in yeast delineated by SNAREs." Chemtracts-Biochemistry and Molecular Biology, 11 (1998) : 446-458.

     
     

    McNew, J.A., Sogaard, M., Lampen, N.M., Machido, S., Ye, R.R., Lacomis, L., Tempst, P., Rothman, J.E., and Sollner, T.H. "Ykt6p, a prenylated SNARE essential for endoplasmic reticulum-Golgi transport." J. Biol. Chem., 272 (1997) : 17776-17783.

     
     

    McNew, J.A., Weber, T., Engelman, D.M., Sollner, T.H., and Rothman, J.E. "The length of the flexible SNAREpin juxtamembrane region is a critical determinant of SNARE-dependent membrane fusion." Mol. Cell, 4 (1999) : 415-421.

     
     

    Nickel, W., Weber, T., McNew, J.A., Parlati, F., Sollner, T.H., and Rothman, J.E. "Content mixing and membrane integrity during membrane fusion driven by pairing of isolated v-SNAREs and t-SNAREs." Proc. Natl. Acad. Sci. USA, 96 (1999) : 12571-12576.

     
     

    Parlati, F., Weber, T., McNew, J.A., Westermann, B., Sollner, T.H., and Rothman, J.E. "Rapid and efficient fusion of phospholipid vesicles with the alpha-Helical core domain of a SNARE complex in the absence of an N-terminal regulatory domain." Proc. Natl. Acad. Sci. USA, 96 (1999) : 12565-12570.

     
     

    Weber, T., Zemelman, B.V., McNew, J.A., Westermann, B., Gmachl, M., Parlati, F., Sollner, T.H, and Rothman, J.E. "SNAREpins: Minimal machinery for membrane fusion." Cell, 92 (1998) : 759-772.

     
     Book chapters
     

    Rodkey, T.R., and McNew, J.A. "SNARE protein effectors." SNARE Proteins (2007)

     
     

    Scott, B.L., Van Komen, J.S., Liu, S., Weber, T., Melia, T. J., and McNew, J. A. "A liposome fusion assay to monitor intracellular membrane fusion machines." Methods in Enzymology, 372 (2003) : 274-300.

     
    Presentations
     Invited Talks
     

    "Cellular aspects of Hereditary Spastic Paraplegia." Spastic Paraplegia Foundation Conference, St. Louis, MO. (June 15, 2013)

     
     

    "ER membrane fusion by the GTPase atlastin." The Physiology of the Endoplasmic Reticulum (ER) : Function and Dysfunction, EMBO Conference Series, Girona, Spain. (October 26, 2014)

     
     

    "ER membrane fusion by the GTPase atlastin." Department of Biology and Biochemistry, University of Houston, Houston, TX. (September 11, 2013)

     
     

    "ER membrane fusion by the GTPase atlastin." Department of Cell and Developmental Biology, The University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC. (April 29, 2013)

     
     

    "ER membrane fusion by the GTPase atlastin." Department of Biological Sciences, The University of North Texas, Denton, TX . (April 30, 2012)

     
     

    "Molecular analysis of membrane fusion by the GTPase atlastin." Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Saint Louis, MO. (January 25, 2012)

     
     

    "Characterization of Atlastin-mediated fusion in vitro." Molecular Basis for Biological Membrane Organization and Dynamics Keystone Symposia, Snowbird, Utah. (January 2010)

     
     

    "Molecular analysis of homotypic membrane fusion by the GTPase atlastin." Biochemistry of Membrane Traffic: Secretory and Endocytic Pathways, American Society for Biochemistry and Molecular Biology Special Symposia, Lake Tahoe, CA. (October 2010)

     
     

    "Regulation of SNARE-mediated membrane fusion." Department of Molecular Medicine, Cornell University School of Veterinary Medicine, Ithaca, NY. (April 10, 2008)

     
     

    "Regulation of SNARE-mediated Membrane Fusion." FASEB Summer Research Conference on Glucose Transporter Biology, Snowmass, Colorado. (August 4-9, 2007)

     
     

    "Regulation of SNARE-mediated Membrane Fusion." Molecular Membrane Biology Gordon Research Conference, Andover, New Hampshire. (July 8-13, 2007)

     
     

    "Regulation of SNARE-mediated Membrane Fusion." Biophysical Society, Baltimore, Maryland. (March 3-7, 2007)

     
     

    "Regulation of SNARE-mediated Membrane Fusion." Department of Biochemistry & Molecular Biology, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma. (February 21, 2007)

     
     

    "Mechanisms of Protein Mediated Membrane Fusion." 2nd Annual Rice Institute of Biosciences and Bioengineering Symposia, Rice University, Houston, Texas. (July 13, 2006)

     
     

    "Regulation of SNARE-Mediated Membrane Fusion." Department of Biochemistry & Cell Biology, Rice University, Houston, Texas. (August 21, 2006)

     
     

    advisor.  "A Novel SNARE Complex Implicated in Vesicular Traffic to the Prevacuolar Compartment." International Conference on Yeast Genetic and Molecular Biology, Bratisalva, Slovakia. (August 7-12, 2005) With B. Doneske, K. Wilson and T. Rice-Stitt

     
     

    "An Intramolecular t-SNARE Complex Functions in vivo Without the Syntaxin N-terminal Regulatory Domain." Yeast Cell Biology, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. (August 18, 2005)

     
     

    "Regulation of SNARE-mediated Membrane Fusion during Exocytosis in S. cerevisiae." Department of Biology, University of Utah. (September, 8 2005)

     
     

    "SNARE Proteins Fuse Membrane via a Hemifusion Intermediate." Rice Membrane Biophysics Group, Department of Physics, Rice University, Houston, Texas. (June 20, 2005)

     
     

    "Molecular Mechanisms of Protein-mediated Membrane Fusion." Integrated Graduate Education and Research Training Program, Rice University. (July 13, 2004)

     
     

    "Molecular Mechanisms of SNARE-mediated Membrane Fusion." Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York. (January 15, 2004)

     
     

    "Regulation of Exocytosis in S. cerevisiae." Cellular Biochemistry and Biophysics Program, Sloan Kettering Institute, New York, New York. (January 18, 2004)

     
     

    "Molecular Mechanisms of Exocytosis in S. cerevisiae." Department of Microbiology and Molecular Genetics, University of Texas-Houston, Houston, Texas. (April 3, 2003)

     
     

    "Molecular Mechanisms of Protein Mediated Membrane Fusion." Frontiers in Biology: Minisymposium on Interdisciplinary Biology at Rice University, Rice University. (January 10, 2003)

     
     

    "Molecular Mechanisms of Protein Mediated Membrane Fusion." Gulf Coast Consortia Membrane Biology Retreat, Battleship Texas. (September 13, 2003)

     
     

    "Mutational Analysis of the S. cerevisiae Plasma Membrane t-SNARE Sso1p." Houston Area Yeast Group, University of Texas-Houston, Houston, Texas. (February 11, 2003)

     
     

    "Proteomic analysis of cell-cell fusion during mating in S. cerevisiae." American Society for Cell Biology, San Francisco, CA. (December 13, 2003)

     
     

    "Mechanism of NIH-1 Fusion and Potential Therapeutic Intervention." Tanox Inc. Meeting, Rice University, Houston, Texas. (September 26, 2002)

     
     

    "Molecular Mechanisms of SNARE-Mediated Membrane Fusion." Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas. (October 9, 2002)

     
     Lectures
     

    "The Role of Complexin in Neuronal SNARE Fusion." IGERT Undergraduate Program, Rice University. (August 6, 2004) With J.R. Schaub, B.L. Scott, and J.S. Van Komen

     
     Other
     

    "Molecular Mechanisms of SNARE-mediated Membrane Fusion." Department of Pharmacology and Integrative Biology, The University of Texas-Houston, Houston, Texas. (April 2001)

     
     

    "Molecular Mechanisms of SNARE-mediated Membrane Fusion." Department of Biology, Texas A&M University, College Station, Texas. (March, 2001)

     
     

    "Molecular Mechanisms of SNARE-mediated Membrane Fusion." Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas. (February, 2001)

     
     

    "Molecular Mechanisms of SNARE-mediated Membrane Fusion." Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas. (February, 2001)

     
     

    "Molecular Mechanisms of SNARE-dependent Membrane Fusion." Department of Biochemistry & Cell Biology, Rice University, Houston, Texas. (February 10, 2000)

     
     Posters
     

    "Munc18a scaffolds SNARE assembly to promote membrane fusion." Molecular Biology of the Cell, American Society for Cell Biology, San Diego, CA. (December 2008) With Rodkey, T.L., Liu, S., Barry, M., and McNew, J.A.

     
     

    lab director.  "Interaction Between the Munc18 and Syntaxin1a Stimulates Membrane Fusion." Alliances for Graduate Education and the Professoriate (AGEP) Banquet, (Aug. 9, 2007) With Rodriguez, A., and McNew, J.A.

     
     

    "An Intramolecular t-SNARE Complex Functions in vivo without the Syntaxin N-terminal Regulatory Domain." Molecular Membrane Biology Gordon Research Conference, Andover, New Hampshire. (July 10-14, 2005) With J. Van Komen, X. Bai and B. Scott

     
     

    "Examination of Complexin Function during SNARE-Mediated Fusion in vitro." Pfizer Summer Undergraduate Research Symposium, Groton, Connecticut. (September 29-30, 2005) With J. Schaub and J. Liao

     
     

    "Examination of Complexin Function during SNARE-Mediated Fusion in vitro." American Society for Cell Biology, San Francisco, California. (December 11, 2005) With J. Schaub and J. Liao

     
     

    "Proteomic Analysis of Cell-Cell Fusion during Mating in S. cerevisiae." American Society for Cell Biology, San Francisco, California. (December 11, 2005) With X. Bai, and S. Lui

     
     

    "Gos1p, Pep12p, Sec9p - A Novel SNARE Complex." NIH biotechnology/NSF IGERT retreat, Rice University. (January 10, 2004) With B. Doneske, T. Rice-Stitt, and K. A. Wilson

     
     

    "Sec1p Binding in vitro in S. cerevisiae." Rice Undergraduate Research Symposium, Rice University. (April 16, 2004) With N. Parikshak, B. L. Scott, J. S. Van Komen

     
     

    "Sec1p Directly simulates SNARE-mediated Membrane Fusion." Lysosomes and Endosomes Gordon Research Conference, Andover, New Hampshire. (June 27 - July 2, 2004) With B.L. Scott, J.S. Van Komen, H. Irshad, S. Liu, and K.A. Wilson

     
     

    "The Role of Complexin in Drosophila Neuronal SNARE Fusion." Rice Undergraduate Research Symposium, Rice University. (April 16, 2004) With J.R. Schaub, B.L. Scott, and J.S. Van Komen

     
     

    "Analysis of Mutations in the Juxtamembrane Region of the S. cerevisiae Plasma Membrane t-SNARE Sso1p." International Congress of Biochemistry and Molecular Biology, Toronto, Canada. (July 20-24, 2003) With J.S. Van Komen, B.L. Scott, and S. Liu

     
     

    "Analysis of mutations in the juxtamembrane region of the S. cerevisiae plasma membrane t-SNARE Sso1p." American Society for Cell Biology, San Francisco, CA. (December 13-17) With J.S. Van Komen and B.L. Scott,

     
     

    "Mutational Analysis of the S. cerevisiae Plasma Membrane t-SNARE Complex." American Society for Cell Biology, San Francisco, California. (December 14-18, 2002) With Van Komen, J.S., Scott, B.L., and Liu, S.

     
     

    "Regulation of Membrane Fusion by the Membrane-proximal Coil of the t-SNARE During Zippering of SNAREpins." American Society for Cell Biology, San Francisco, California. (December 14-18, 2002) With Melia, T.J., Weber, T., McNew, J.A., Fisher, L.E., Johnston, R.J., Parlati, F., Mahal, L.K., and Sollner, T.H.

     
     

    "Sec1p Function in Yeast Exocytosis." American Society for Cell Biology, San Francisco, California. (December 14-18, 2002) With Scott, B.L., Liu, S., and Van Komen, J.S.

     
    Editorial Positions
     Member of the Editorial Board, F1000 Research. (2012 - 2012)

    Supervised Theses & Dissertations
     Miles Scotcher, Ph.D. Genetic Factors Affecting the Regulation of Solventogenesis in Clostridium acetobutylicum ATCC 824. (2004) (Thesis Committee Member)

     Jeffery Myers, Ph.D. Human UP1 as a Model for Understanding Purine Recognition in the Family of Proteins Containing the RNA Recognition Motif (RRM). (2004) (Thesis Committee Member)

     Dennis Lee, Ph.D. Plant Responses to Environmental Stimuli: A Screen for Mutants in TCH4 Expression Behavior, and a Full-genome Assessment of Touch and Darkness Inducibility. (2004) (Thesis Committee Member)

     Brenton Scott, Ph.D. Regulation of SNARE-Mediated Membrane Fusion by Sec1/Munc18 (SM) Proteins. (2005) (Thesis Director)

     David Ji, M.A. Elucidating the Mechanism of Action of Lmo4 in Vertebrate Eye Development. (2005) (Thesis Committee Member)

     A. Raquel Adham, Ph.D. Molecular and Genetic Analysis of IBA Homeostasis and Action in Arabidopsis. (2005) (Thesis Committee Member)

     Andrew Woodward, Ph.D. Genes, Organelles, and Molecules that Influence Plant Development Through Auxin Regulation. (2005) (Thesis Committee Member)

     Jeff VanKomen, Ph.D. Regulatory Elements of Sso1p and Their Effect on SNARE Mediated Membrane Fusion. (2005) (Thesis Director)

     Jamie Becnel, Ph.D. Xyloglucan Endotransglucosylase/Hydrolases of Arabidopsis: Expression and Function of a Gene Family. (2006) (Thesis Committee Member)

     Blair Doneske, Ph.D. Regulation of Neuronal SNARE Complexes by Complexin and Ca++-Synaptotagmin. (2007) (Thesis Director)

     Xiaoyang Bai, Ph.D. The Molecular Mechanism of Membrane Fusion in the Yeast S. cerevisiae. (2007) (Thesis Director)

     Song Liu, Ph.D. In Vitro Fusion Catalyzed by the Sporulation-Specific t-SNARE Light-chain Spo20p Is Stimulated by Phosphatidic Acid. (2007) (Thesis Director)

     Catherine McCollum, Ph.D. Zebrafish lmo4b Restricts the Size of the Embryonic Forebrain and Eyes Through six3b and rx3, and is Regulated by Extracellular Signals in Patterning the Eye Along the Proximodistal Axis. (2007) (Thesis Committee Member)

     Jeanne Rasbery, Ph.D. Genetic and Biochemical Characterization of the Squalene Epoxidase Gene Family in . (2007) (Thesis Committee Member)

     Elizabeth Poggio, M.A. Molecular Genetic Analyses of Plant Peroxisome Functions. (2008) (Thesis Committee Member)

     Todd Jaszewski, Ph.D. Specificity of Transmembrane Domain Self-association by Mutagenesis and Protein Design. (2008) (Thesis Committee Member)

     Tanvi Desai, Ph.D. Characterization of Structure and Function Relationship between Domains of the ER Membrane Protein Atlastin. (2013) (Thesis Director)

     Avani Verma, Ph.D. Characterization of Proteins Involved in Intracellular Membrane Fusion- Atlastin and Munc18c. (2013) (Thesis Director)

     Joseph Faust, Ph.D. Peroxisome Biogenesis in Drosophila melanogaster: Protein Trafficking, Lipid Metabolism, and Muscle Function. (2013) (Thesis Director)

     Miguel A Betancourt, PhD In vitro structure-function analysis of Drosophila atlastin. (Thesis Director)

    Awards, Prizes, & Fellowships
     Simmons Foundation Award, (2013)

     Member, American Society for Cell Biology,

     Ad hoc Reviewer, National Science Foundation,