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Scholarly Interest Report
         
John S. Olson
Professor
Ralph and Dorothy Looney Professor of Biochemistry and Cell Biology
 
e-mail:olson@rice.edu
 
  • B.S. Chemistry (1968) University of Illinois, Urbana
  • Ph.D. Biochemistry (1972) Cornell University, Ithaca, New York
 
Primary Department
   Department of BioSciences
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Department Affiliations
 
  • Institute of Biosciences and Bioengineering
  • Keck Center for Quantitative Biomedical Sciences
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    Other Affiliations
     
  • Baylor College of Medicine
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    Research Areas
     Biochemical, biophysical, and physiological properties of myoglobins, hemoglobins, and red blood cells. Genetic engineering of heme protein based blood substitutes. Application of ultrafast mixing and laser kinetic techniques to biological problems.
     
    Research Statement
     

    Our laboratory has been using biochemical, biophysical, chemical, and chemical engineering approaches to examine the fundamental processes involved in oxygen transport and storage in mammalian circulatory systems. We are directing two major research programs, one involving basic molecular biophysics and the other involving the design of O2 delivery pharmaceuticals. (1) Dynamics of O2 binding to heme proteins. The roles of specific amino acids and structural motifs in regulating the kinetics and affinity of O2 binding and the resistance of myoglobins olson_workand hemoglobins to denaturation are being identified by: UV-visible, vibrational, CD, fluorescence, and NMR spectroscopies; rapid mixing and ultrafast laser photolysis kinetic techniques; X-ray crystallography, and various computational methods. (2) The design of Hb-based blood substitutes. Rational and random mutagenesis techniques are being used to optimize seven key properties of extracellular hemoglobin: (a) moderately low O2 affinity and high cooperativity, (b) discrimination against CO binding, (c) high rates of O2 exchange, (d) low rates of reaction with NO, (e) resistance to autooxidation and reactions with H2O2, (f) high affinity for heme, and (g) resistance to denaturation. Our mechanisms are being used by Baxter Hemoglobin Therapeutics, Boulder, CO (formerly Somatogen, Inc.) to develop second generation blood substitutes.

     
    Selected Publications
     Refereed articles
     

    Esquerra, R.M., Bibi, B.M., Tipgunlakant, P., Birukou, I., Soman, J.,Olson,J.S., Kliger, D.S., Goldbeck, R. A. "The Role of Heme Pocket Water in Allosteric Regulation of Ligand Reactivity in Human Hemoglobin." Biochemsitry, submitted (2016) : bi-2016-00081z.Submitted

     
     

    Da, Q., Teruya, M., Guchhait, P., Teruya, J., Olson, J.S., and Cruz, M.A. "Free Hemoglobin modulates von Willebrand factor-mediated platelet adhesion in vitro: implications on circulatory devices." Blood, 126 (2015) : 2338-2341.

     
     

    Mueller, C., Marx, A., Epp, S. W., Zhong, Y., Kuo, A., Balo, A. R., Soman, J., Schotte, F., Lemke, H. T., Owen, R. L., Pai, E. F., Pearson, A. R., Olson, J. S., and Anfinrud, P.A., Ernst, O. P., and Miller, R. J. D. "Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography." Structural Dynamics, 2 (2015) : 054302-1 to 15.

     
     

    Samuel, P. P., Phillips, G. N. Jr., Smith, L. P., and Olson, J. S. "Apoglobin stability is the major factor governing both cell-free and in vivo expression of holomyoglobin." J. Biol. Chem., 290 (2015) : 23479-95.

     
     

    Sjodt, M., Macdonald, R., Spirig, T., Chan, A. H., Dickson, C. F., Fabian, M., Olson, J. S., Gell, D. A., and Clubb, R. T. "The NMR The NMR Model of the 38.8 kDa Tri-Domain IsdH Protein from Staphylococcus aureus Suggests that it Adaptively Recognizes Human Hemoglobin." J. Mol. Biol., Epub (2015) : 10.1016/j.jmb.2015.02.008.

     
     

    Mollan, T. L., Jia, Y., Banerjee, S., Wu, G., Kreulen, T., Tsai, A-L., Olson, J. S., Crumbliss, A. L., and Alayash, A. I. "Redox Properties of Human Hemoglobin in Complex with Fractionated Dimeric and Polymeric Human Haptoglobin." Free Rad. Biol. Med., 69 (2014) : 265-277.

     
     

    Strader, M. B., Hicks, W. A., Kassa, T., Singleton, E. W., Soman, J. Olson, J. S., Weiss, M. J., Mollan, T., L., Wilson, M. T., and Alayash, A "A Post-translational transformation of methionine to aspartate is catalyzed by heme iron and driven by peroxide: A novel subunit specific mechanism in hemoglobin." J. Biol. Chem., 289 (2014) : 22342-57.

     
     

    Boechi, L., Arrar, M., Marti, M. A., Olson, J. S., Roitberg, and A. E., Estrin, D. A. "Hydrophobic effect drives oxygen uptake in myoglobin via histidine E7." J. Biol. Chem., 288 (2013) : 6754-62.

     
     

    Dickson, C.F., Rich, A. M., D’Avigdor, W. M. H., Collins, D., Lowry, J. Mollan, T. L., Khandros, E., Olson, J. S., Weiss, M. J., Mackay, J. P., Lay, P. A., and Gell, D. A. "α-Hemoglobin Stabilizing Protein Perturbs the Proximal Heme Pocket of Oxy-α-Hemoglobin and Weakens the Iron-Oxygen Bond." J. Biol. Chem., 288 (2013) : 19986-20001.

     
     

    Mollan, T. L., Banerjee, S., Wu, G., Parker Siburt, C. J., Tsai, A-L., Olson, J. S., Weiss, M. J., Crumbliss, A. L., and Alayash, A. I. "Alpha-Hemoglobin Stabilizing Protein (AHSP) Markedly Decreases the Redox Potential and Reactivity of α Subunits of Human HbA with Hydrogen Peroxide." J. Biol. Chem., 288 (2013) : 4288-98.

     
     

    Nienhaus, K., Olson, J.S., and Nienhaus, G.U. "An Engineered Heme-Copper Center in Myoglobin: CO Migration and Binding." Biochim. Bioophys. Acta. Proteins, 1834 (2013) : 1824-31.

     
     

    Schotte, F., Hyun Sun Cho, H. S.,.Soman, J., Wulff, M., Olson, J. S., and Anfinrud, P. A. "Real-time tracking of CO migration and binding in the alpha and beta subunits of human hemoglobin via 150 ps time-resolved Laue crystallography." Chem. Physics, 422 (2013) : 98-106.

     
     

    Varnado, C. L., Mollan, T. L., Birukou, I., Smith, B. J. Z., Henderson, D. P., and Olson, J. S. "Development of Recombinant Hemoglobin-Based Oxygen Carriers." Antioxid. Redox Signal.: Forum Review, 18 (2013) : 2314-28.

     
     

    Zheng, W., Olson, J. S., Vakharia, V., and Tao, Y. J. "The crystal structure and RNA-binding of an orthomyxovirus nucleoprotein.." Plos Pathogens, 9 (2013) : e1003624.

     
     

    Ekworomadu, M., Poor, C.B., Owens C.P., Balkabasi, M., Fabian, M., Olson, J. S., Murphy, F., Balkabasi, E., Honsa, E., He, C., Celia W. Goulding, C.W., and Maresso, A. W. "Differential Function of Lip Residues in the Mechanism and Biology of an Anthrax Hemophore." PloS Pathogens, 8(3) (2012) : e1002559.

     
     

    Khandros, E., Mollan, T. L., Yu, X., Wang, X., Yao, Y., D'Souza, J. D., Gell, D. A., Olson, J. S., and Weiss, M. J. "Insights Into Hemoglobin Assembly Through In Vivo Mutagenesis of Alpha-Hemoglobin Stabilizing Protein." J. Biol. Chem., 287 (2012) : 11325-11337.

     
     

    Mollan, T. L., Abraham, B., Strader, M. B., Jia, Y. Lozier, J. N., Olson, J. S., and Alayash, A. I. "Familial Secondary Erythrocytosis Due to Increased Oxygen Affinity Is Caused by Destabilization of the T State of Hemoglobin Brigham (alpha2beta2(2Pro100Leu))." Protein Sci., 287 (2012) : 11338-11350.

     
     

    Mollan, T. L., Khandros, E., Weiss, M. J., and Olson, J. S. "The Kinetics of α-Globin Binding to α-Hemoglobin Stabilizing Protein (AHSP) Indicate Preferential Stabilization of a Hemichrome Folding Intermediate." J. Biol. Chem., 287 (2012) : 11338-11350.

     
     

    Salter, M. D., Blouin, G. C., Singleton, E. W., Dewilde, S., Moens, L., Pesce, A., Nardini, M., Bolognesi, M., and Olson, J. S. "Determination of Ligand Pathways in Globins: Apolar Tunnels versus Polar Gates." J. Biol. Chem., 287 (2012) : 33163-33178.

     
     

    Tsai, A-L., Martin, E., Berka, V., and Olson, J. S. "How do heme-protein sensors exclude oxygen: Lessons learned from cytochrome c', Ns H-NOX and soluble guanylyl cyclase." Antioxid. Redox Signal.: Forum Review, 17 (2012) : 1246-63.

     
     

    Tsai, A. L., Berka, V., Martin, E., and Olson, J. S. "A "Sliding-Scale Rule" for Selectivity between NO, CO and O2 by Heme Protein Sensors." Biochemistry, 51 (2012) : 172-86.

     
     

    Birukou, I., Maillett, D. H., Birukova, A., and Olson, J. S. "Modulating Distal Cavities in the alpha and beta Subunits of Human HbA Reveals the Primary Ligand Migration Pathway." Biochemistry, 50 (2011) : 7361-74.

     
     

    Birukou, I., Soman, J., and Olson, J. S. "Blocking the Gate to Ligand Entry in Human Hemoglobin." J. Biol. Chem., 286 (2011) : 10515-52.

     
     

    Crowley, M. A., Mollan, T. L., Abdulmalik, O. Y., Butler, A. D., Goodwin, E. F., Sarkar, A. Stolle, C. A., Gow, A. J., Olson, J. S., Weiss, M. J. "Hemoglobin Toms River – A Variant Causing Neonatal Cyanosis and Anemia.." New Eng. J. Med, 364 (2011) : 1837-43.

     
     

    Honsa, E. S., Fabian, M., Cardenas, A. M., Olson, J. S., and Maresso, A. W. "The five near-iron transporter (NEAT) domain anthrax hemophore, IsdX2, scavenges heme from hemoglobin and transfers heme to the surface protein IsdC."  J. Biol. Chem., 286 (2011) : 33652-60.

     
     

    Lukianova-Hleb, E. Y., Oginsky, A. O., Olson, J. S., and Lapotko, D. O. "Short laser pulse-induced irreversible photothermal effects in red blood cells." Lasers in Surgery & Medicine, 43 (2011) : 249-60.

     
     

    Pesce, A., Nardini, M., Dewilde, S., Capece, L., Martí, M.A., Congia, S., Salter, M.D., Blouin, G.C., Estrin,D. A., Ascenzi, P., Moens, L., Bolognesi, M., and Olson, J.S. "Ligand Migration in the Apolar Tunnel of Cerebratulus lacteus Mini-Hemoglobin.." J. Biol. Chem., 286 (2011) : 5347-58.

     
     

    Bianchetti, C. M., Blouin, G. C., Bitto, E., Olson, J. S., and Phillips, G. N. Jr. "The Structure and NO Binding Properties of the Nitrophorin-like Heme-binding Protein from Arabidopsis thaliana gene locus Atlg79260.1." Proteins, 78 (2010) : 917-931.

     
     

    Birukou, I., Schweers, R. L., and Olson, J. S. "The Distal Histidine Stabilizes Bound O2 and Acts as Gate for Ligand Entry n Both Subunits of Human HbA." J. Biol. Chem., 285 (2010) : 8840-54.

     
     

    Blouin, G., Schweers, R. L., and Olson, J.S. "Alkyl Isocyanides Serve as Transition State Analogs for Ligand Entry and Exit in Myoglobin." Biochemistry, 49 (2010) : 4987-4997.

     
     

    Blouin, G., and Olson, J.S. "The Stretching Frequencies of Bound Alkyl Isocyanides Indicate Two Distinct Ligand Orientations within the Distal Pocket of Myoglobin." Biochemistry, 49 (2010) : 4968-4976.

     
     

    Culbertson, D. S. and Olson, J. S. "The Role of Heme in the Unfolding and Assembly of Myoglobin." Biochemistry, 49 (2010) : 6052-6063.

     
     

    Esquerra, R. M., Lopez-Pena, I., Tipgunlakant, P., Birukou, I., Nguyen, R. L., Soman, J., Olson, J.S., Kliger, D.S., and Goldbeck, R. A. "Kinetic Spectroscopy of Heme Hydration and Ligand Binding in Myoglobin and Isolated Hemoglobin Chains: An Optical Window into the Functional Dynamics of Water in the Heme Pocket." Physical Chemistry Chemical Physics, 12 (2010) : 10270-8.

     
     

    Mollan, T., Yu, X., Weiss, M.J., and Olson, J.S. "The role of alpha-hemoglobin stabilizing protein in redox chemistry.." Antioxidants and Redox Signaling, 12 (2010) : 219-231.

     
     

    Pena, M., Davlieva, M., Olson, J. S. Bennett, M., and Shamoo, Y. "Evolutionary fates within a microbial population highlight an essential role for reversible and irreversible protein folding during natural selection." Molecular Systems Biology, on-line journal (2010) : Article 387.

     
     

    Smith, R. D., Blouin, G., Johnson, K. A., Phillips, G.N.Jr., and Olson, J.S. "Straight-Chain Alkyl Isocyanides Open the Distal Histidine Gate in Crystal Structures of Myoglobin." Biochemistry, 49 (2010) : 4977-4986.

     
     

    Sodatova, A.V., Ibrahim, M., Olson, J.S., and Spiro, T.G. "New light on NO bonding in Fe(III) heme proteins from resonance Raman spectroscopy and DFT modeling." J Amer Chem Soc, 132 (2010) : 4614-4625.

     
     

    Tarlovsky, Y., Fabian, M., Olson, J.S., and Maresso, A. W. "An S-Layer Homology Protein Mediates Heme Delivery to the Isd system in the Bacillus anthracis." J. Bacteriology, 192 (2010) : 3503-3511.

     
     

    Tsai, A-L., Berka, V., Martin, F., Ma, X., van den Akker, F., Fabian, M., and Olson, J. S. "Is Nostoc H-NOX a Gaseous Sensor or a Redox Switch?." Biochemistry, 49 (2010) : 6587-6599.

     
     

    Aranda, R. 4th, Cai, H., Worley, C.E., Levin, E.J., Li, R., Olson, J.S., Phillips, G.N. Jr., and Richards, M.P. "Structural analysis of bovine, trout IV, and perch hemoglobin: Effects of the heme pocket environment on autooxidation and hemin loss rates." Proteins, 75 (2009) : 217-230.

     
     

    Asmundson, A. L., van der Walde, A., Lin, D. H, Olson, J. S., and Anthony-Cahill, S. J. "Co-Expression of Human α- and Circularly Permuted β-Globins Yields a Hemoglobin with Normal R state but modified T state Properties." Biochemistry, 48 (2009) : 5456-5465.

     
     

    Fabian, M., Solomaha, E., Olson, J.S., and Maresso, A. "Heme transfer to the bacterial cell envelope occurs via a secreted hemophore in the gram-positive pathogen Bacillus anthracis." J Biol Chem, 284 (2009) : 32138-32146.

     
     

    Golbeck, R.S., Pillsbury, M.L., Jensen, R.A., Mendoza, J.L., Nguyen, R.L., Olson, J.S., Soman, J., Kliger, D.S., and Esquerra, R.M. "Optical detection of disordered water within a protein cavity." J Amer Chem Soc, 131 (2009) : 12265-12272.

     
     

    Smagghe, B. J., Hoy, J. A., Percifield, R., Kundu, S., Hargrove, M. S., Sarath, G., Hilbert, J-L., Watts, R. A., Dennis, E. S., Peacock, W. J., Dewilde, S., Moens, L., Blouin, G. C., Olson, J. S., and Appleby, C. "Correlations between Oxygen Affinity and Sequence Classifications of Plant Hemoglobins." Biopolymers, 91 (2009) : 1083-1096.

     
     

    Yu, X., Mollan, T. L., Butler, A., Gow, A. J., Olson, J. S., and Weiss M. J. "Analysis of Human α Globin Gene Mutations that Impair Binding to the Alpha Hemoglobin Stabilizing Protein (AHSP)." Blood, 113 (2009) : 5961 - 5969.

     
     

    Graves, P.E, Henderson, D.P., Horsman, J.M., Solomon, B.J., and Olson, J.S. "Enhancing stability and expression of recombinant human hemoglobin in E. coli: Progress in the development of a recombinant HBOC source." Biochim. Biophys. Acta, 1784 (2008) : 1471-1479.

     
     

    Hussain, F., Olson, J.S., and Wittung-Stafshede, P. "Conserved residues modulate copper release in human copper chaperone Atox1." Proceedings of the National Academy of Sciences, USA, 105 (2008) : 11158-11163.

     
     

    Maillett, D.H., Simplaceanu, V., Shen, T.J., Ho, N.T., Olson, J.S. and Ho, C. "Interfacial and distal-heme pocket mutations exhibit additive effects on the structure and function of hemoglobin." Biochemistry, 47 (2008) : 10551-10563.

     
     

    Salter, M.D., Nienhaus, K., Nienhaus, G.U., Dewilde, S., Moens, L., Pesce, A., Nardini, M., Bolognesi, M., and Olson, J.S. "The apolar channel in cerebratulus lacteus hemoglobin is the route for O2 entry and exit." J. Biol. Chem., 283 (2008) : 35689-35702.

     
     

    Villarreal, D.M., Phillipds, C.L., Kelley, A.M., Villarreal, S., Villaloboz, A., Hernandez, P., Olson, J.S., and Henderson, D.P. "Enhancement of recombinant hemoglobin production in Escherichia coli BL21(DE3) containing the Plesiomonas shigelloides heme transport system." Appl. Environ. Microbiol., 74 (2008) : 5854-5856.

     
     

    Zhu, H., Xie, G., Liu, M., Olson, J.S., Fabian, M., Dooley, D.M., and Lei, B. "Pathway for heme uptake from human methemoglobin by the iron-regulated surface determinants system of Staphylococcus aureus." J. Biol. Chem., 283 (2008) : 18450-18460.

     
     

    Aranda, R., Worley, C.E., Liu, M., Bitto, E., Cates, M.S., Olson, J.S., Lei, B., and Phillips, G.N. Jr. "Bis-methionyl coordination and heme stacking in the crystal structure of the heme-binding domain of the streptococcal cell surface protein Shp." J. Mol. Biol., 374 (2007) : 374-383.

     
     

    Deng, P., Nienhaus, K., Palladino, P., Olson, J.S., Blouin, G., Moens, L., Dewilde, S., Gevens, E., and Nienhaus, G.U. "Transient ligand docking sites in Cerebratus lacteus mini-hemoglobin." Gene, 398 (2007) : 208-223.

     
     

    Mocny, J.C., Olson, J.S., and Connell, T.D. "Passively released heme from hemoglobin and myoglobin is a potential source of nutrient iron for Bordetella bronchiseptica." Infection & Immunity, 75 (2007) : 4857-4866.

     
     

    Olson, J.S., Soman, J., and Phillips, G.N. Jr, "Ligand pathways in myoglobin: A review of Trp cavity mutations." IUBMB Life, 59 (2007) : 552-562.

     
     

    Ran, Y., Zhu, H., Liu, M., Fabian, M., Olson, J.S., Aranda, R. IV., Phillips, G.N., Jr., Dooley, D.M., and Lei, B. "Bis-methionine coordination in Shp facilitates rapid heme transfer to HtsA of the HtsABC transporter in a plug-in mechanism." J. Biol. Chem., 282 (2007) : 31380-31388.

     
     

    Gardner, P.A., Gardner, A.M., Brashear, W.T., Suzuki, T., Hvitved, A.N., Setchell, K.D.R., and Olson, J.S. "Hemoglobins Deoxygenate NO with High Fidelity." Journal of Inorganic Biochemistry, 100 (2006) : 542-550.

     
     

    Goldbeck, R.A., Bhaskaran, S., Ortega, C., Mendoza, J., Olson, J. Soman, J., Kliger, D.S., and Esquerra, R.M. "Water and ligand entry in myoglobin: Assessing the speed and extent of heme pocket hydration after CO photodissociation." Proc. Natl. Acad. Sci., 103 (2006) : 1254-1259.

     
     

    Mocny, J.C., Olson, J.S., and Connell, T.D. "Iron acquisition by Bordetella bronchiseptica depends upon a spontaneous release of heme from hemoglobin and myoglobn." Infection & Immunity (2006) In Revision

     
     

    Nygaard, T.K., Blouin, G.C., Liu, M., Fukumura, M., Olson, J.S., Fabian, M., Dooley, D.M., and Lei, B. "The mechanism of heme transfer from the Streptococcal cell surface protein Shp to HtsA of the HtsABC transporter." J. Biol. Chem., 281 (2006) : 20761-20771.

     
     

    Zhou, S., Olson, J.S., Fabian, M., Weiss, M.J., and Gow, A.J. "Biochemical fates of alpha hemoglobin bound to alpha hemoglobin stabilizing protein (AHSP)." J. Biol. Chem., 281 (2006) : 32611-32618.

     
     

    Dantsker, D., Roche, C., Samuni, U., Blouin, G., Olson, J.S., and Friedman, J. "The position 68(E11) side chain in myoglobin regulates ligand capture bond formation with the heme iron, and internal movement into the Xe cavities." J. Biol. Chem., 280 (2005) : 38740-38755.

     
     

    Helmick, R.A., Fletcher, A.E., Gardner, A.M., Gessner, C.R., Hvitved, A.N., Gustin, M.C., and Gardner, P.R. "Imidazole antibiotics inhibit the nitric oxide dioxygenase function of microbial flavohemoglobin." Antimicrob. Agents Chemother, 49 (2005) : 1837-1843.

     
     

    Ionascu, D., Gruis, F., Ye, X., Yu, A., Rosca, F., Beck, C., Demidov, A., Olson, J.S., and Champion, P.M. "Temperature dependent studies of NO recombination to heme and heme proteins." J. Amer. Chem. Soc. (2005) In Press

     
     

    Miranda, J.J.L., Maillett, D.H., Soman, J., and Olson, J.S. "Thermoglobin, oxygen-avid hemoglobin in a eubacterial hyperthermophile." J. Biol. Chem., 280 (2005) : 36754-36761.

     
     

    Nienhaus, K., Olson, J.S., Franzen, S., and Nienhaus, G.U. "The origin of stark splitting in the initial photoproduct state of MbCO." J. Amer. Chem. Soc., 127 (2005) : 40-42.

     
     

    Zhang, W., Olson, J.S., and Phillips, G.N., Jr. "Biophysical, kinetic characterization, and crystallization of HemAT: An aerotaxis transducer from Bacillus subtilis." Biophys. J., 88 (2005) : 2801-2814.

     
     

    Geuens, E., Dewilde, S., Hoogewijs, D., Pesce, A., Nienhaus, K., Olson, J.S., Vanfleteren, J., Bolognesi, M., and Moens, L. "Nerve hemoglobins in invertebrates." IUBMB Journal (2004) In Press

     
     

    Kundu, S., Blouin, G.C., Premer, S.A., Sarath, G., Olson, J.S., and Hargrove, M.S. "TyrB10 inhibits stabilization of bound CO and O2 in soybean leghemoglobin." Biochemistry, 43 (2004) : 6241-6252.

     
     

    Nienhaus, K., Olson, J.S., Franzen, S., Nienhaus, G.U. "The Origin of Stark Splitting in the Initial Photoproduct State of MbCO." J. Amer. Chem. Soc. (2004) In Press

     
     

    Olson, J.S., Foley, E.W., Rogge, C., Tsai, A-L., Doyle, M.P., and Lemon, D.D. "NO Savenging and the hypertensive effect of Hb-based blood substitutes." Free Radical Biol. Med., 36 (2004) : 685-697.

     
     

    Pesce, A., Nardini, M., Ascenzi, P., Geuens, E., Dewilde, S., Moens, L., Bolognesi, M., Riggs, A.F., Hale, A., Deng, P., Nienhaus, G.U., Olson, J.S., and Nienhaus, K. "ThrE11 Regulates O2 affinity in Cerebratulus lacteus minihemoglobin." J. Biol. Chem., 279 (2004) : 33662-33672.

     
     

    Puranik, M., Nielsen, S.B., Youn, H., Bourassa, J.L., Case, M.A., Hvitved, A.N., Tengroth, C., Balakrishnan, G., Thorsteinsson, M.V., Olson, J.S., Roberts, G.P., Groves, J.T., McLendon, G.L., and Spiro, T.G. "Dynamics of carbon monoxide binding to the CooA protein." J. Biol. Chem., 279 (2004) : 21096-21108.

     
     

    Schotte, F., Soman, J., Olson, J.S., Wulff, M., and Anfinrud, P.A. "Picosecond time-resolved X-ray crystallography: Probing protein function in real time." J. Biol. Struct., 147 (2004) : 235-246.

     
     

    Unno, M., Matsui, T., Chu, G.C., Couture, M., Yoshida, T., Rousseau, D.L., Olson, J.S., and Ikeda-Saito, M. "Crystal structure of the dioxygen-bound heme oxygenase from Corynebacterium diphtheriae: Implications for heme oxygenase function." J. Biol. Chem., 279 (2004) : 21055-21061.

     
     

    Nienhaus, K., Deng, P., Olson, J.S., Warren, J.J., and Nienhaus, G.U. "Structural Dynamics of Myoglobin: Ligand Migration and Binding in Valine 68 Mutants." J. Biol. Chem., 278 (2003) : 42532-42544.

     
     

    Schotte, F., Lim, M., Jackson, T.A., Smirnov, A.V., Soman, J., Olson, J.S., Phillips, G.N., Jr., Wulff, M., and Anfinrud, P.A. "Watching a protein as it functions with 150 ps time-resolved X-ray crystallography." Science, 300 (2003) : 1944-1947.

     
     

    Vogel, K.M., Coyle, C.M., Rush III, T.S., Kozlowski, P.M., Williams, R., Spiro, T.G., Dou, Y., Ikeda-Saito, M., Olson, J.S., and Zgierski, M.Z., "FeNO structure in distal pocket mutants of myoglobin from resonance Raman spectroscopy." Biochemistry, 42 (2003) : 4896-4903.

     
     

    Dou, Y., Maillett, D.H., Eich, R.F., and Olson, J.S. "Myoglobin as a model system for designing heme protein based blood substitutes." Biophys. Chem, 98 (2002) : 127-148.

     
     

    Draghi, F., Miele, A.E., Travaglini-Allocatelli, C., Vallone, B., Brunori, M., Gibson, Q.H., and Olson, J.S. "Controlling ligand binding in myoglobin by mutagenesis." J. Biol. Chem., 277 (2002) : 7509-7519.

     
     

    Liong, E.C., Dou, Y., Scott, E.E., Olson, J.S., and Phillips, G.N. Jr. "Water-Proofing the Heme Pocket: the Role of Proximal Amino Acid Side Chains in Preventing Hemin Loss from Myoglobin." J. Biol. Chem., 276 (2001) : 9093-9100.

     
     

    Scott, E.E., Gibson, Q.H., and Olson, J.S. "Mapping Pathways for O2 Entry and Exit from Myoglobin." J. Biol. Chem., 276 (2001) : 5177-5188.

     
     

    Gardner, A.M., Martin, L.A., Gardner, P.R., Dou, Y., and Olson, J.A. "Steady-State and Transient Kinetics of Escherichia coli Nitric Oxide Dioxygenase (Flavohemoglobin): The Tyrosine B10 Hydroxyl Is Essential for Oxygen Binding and Catalysis." J. Biol. Chem., 275 (2000) : 12581-12589.

     
     

    Gardner, P.R., Martin, L.A., Gardner, A.M., Dou, Y., Li, T., and Olson, J.S. "Nitric Oxide Dioxygenase Activity and Function of Microbial Flavohemoglobin." J. Biol. Chem., 275 (2000) : 31581-31587.

     
     

    Hargrove, M.S., Brucker, E.A., Stec, B., Sarath, G., Arrendondo-Peter, R., Klucas, R.V., Olson, J.S., and Phillips, G.N., Jr. "Crystal Structure of a Non-Symbiotic Plant Hemoglobin." Structure, 8 (2000) : 1005-1014.

     
     

    Qui, Y., Maillett, D.H., Knapp, J., Olson, J.S., and Riggs, A.F. "Lamprey Hemoglobin: Structural Basis of the Bohr Effect." J. Biol. Chem., 275 (2000) : 13515-13528.

     
     

    Scott, E.E., Paster, E.V., and Olson, J.S. "The Stabilities of Mammalian Apomyoglobins Vary Over a 600-fold Range and Can Be Enhanced by Comparative Mutagenesis." J. Biol. Chem., 275 (2000) : 27129-27136.

     
     

    Alayash, A.I., Brockner Ryan, B.A., Eich, R.F., Olson, J.S., and Cashon, R.E. "Reactions of Sperm Whale Myoglobin with Hydrogen Peroxide: Effects of Distal Pocket Mutations on the Formation and Stability of the Ferryl Intermediate." J. Biol. Chem., 274 (1999) : 2029-2037.

     
     

    Phillips, G.N., Jr., Teodoro, M., Li, T., Smith, B., Gilson, M.M., and Olson, J.S. "Bound CO Is a Molecular Probe of Electrostatic Potential in the Distal Pocket of Myoglobin." J. Phys. Chem. B, 103 (1999) : 8817-8829.

     
     

    Thorsteinsson, M.V., Bevan, D.R., Potts, M., Dou, Y., Eich, R.F., Hargrove, M.S., Gibson, Q.H., and Olson, J.S. "A Bacterial Hemoglobin with Unusual Ligand Binding Kinetics and Stability Properties." J. Biol. Chem. B, 38 (1999) : 2117-2126.

     
     

    Tomita, T., Hirota, S., Ogure, T., Olson, J.S., and Kitagawa, T. "Resonance Raman Investigation of Fe-N-O Structure of Nitrosylheme in Myoglobin and Its Mutants." J. Phys. Chem., 103 (1999) : 7044-7054.

     
     

    Brucker, E.A., Olson, J.S., Ikeda-Saito, M., and Phillips, G.N., Jr. "Nitric Oxide Myoglobin: Crystal Structure and Analysis of Ligand Geometry." PROTEINS: Structure, Function, Genetics, 30 (1998) : 352-356.

     
     

    Doherty, D.H., Doyle, M.P., Curry, S.R., Vali, R.J., Fattor, T.J., Olson, J.S., and Lemon, D.D. "Rate of Reaction with Nitric Oxide Determines the Hypertensive Effect of Cell-free Hemoglobin." Nature Biotechnology, 16 (1998) : 672-676.

     
     

    Kryzwda, S., Murshudov, G.N., Brzozowski, A.M., Jaskolski, M., Scott, E.E., Klizas, S.A., Gibson, Q.H., Olson, J.S., and Wilkinson, A.J. "Stabilizing Bound O2 in Myoglobin by Valine68(E11) to Asparagine Substitution." Biochemistry, 37 (1998) : 15896-15907.

     
     

    Mansy, S.S., Olson, J.S., Gonzalez, G., and Gilles-Gonzalez, M.A. "Imidazole Is a Sensitive Probe of Steric Hindrance in the Distal Pockets of Oxygen-binding Heme Proteins." Biochemistry, 37 (1998) : 12452-12457.

     
     

    Migita, C.T., Matera, K.M., Ikeda-Saito, M., Olson, J.S., Fujii, H., Yoshimura, T., Zhou, H., and Yoshida, T. "The Oxygen and Carbon Monoxide Reactions of Heme Oxygenase." Biochemistry, 273 (1998) : 945-949.

     
     

    Mukai, M., Nakashima, S., Olson, J.S., and Kitagawa, T. "Time-Resolved UV Resonance Raman Detection of a Transient Open Form of the Ligand Pathway in Tyr64(E7) Myoglobin." J. Phys. Chem., B102 (1998) : 3624-3630.

     
     

    Nakashima, S., Kitagawa, T., and Olson, J.S. "Time-Resolved Resonance Raman Study of Intermediates Generated After Photodissociation of Wild-type and Mutant CO Myoglobins." Chem. Phys., 228 (1998) : 323-336.

     
     

    Nguyen, B.D., Zhao, X., Krishnamurthi, V., La Mar, G.N., Lile, R.A., Brucker, E.A., Phillips, G.N., Jr., Olson, J.S., and Wittenberg, J.B. "Solution and Crystal Structures of a Sperm Whale Myoglobin Triple-Mutant that Mimics the Sulfide Binding Hemoglobin from Lucina pectinata." J. Biol. Chem., 273 (1998) : 9517-9526.

     
     

    Tang, Q., Kalsbeck, W.A., Olson, J.S., and Bocian, D.F. "Disruption of the Heme Iron-Proximal Histidine Bond Requires Unfolding of Deoxymyoglobin." Biochemistry, 37 (1998) : 7047-7056.

     
     

    Unno, M., Christian, J.F., Olson, J.S., and Champion, P.M. "Evidence of Hydrogen Bonding Effects in the Iron Ligand Vibrations of Carbonmonoxy Myoglobin." J. Amer. Chem. Soc., 120 (1998) : 2670-2671.

     
     

    Unzai, S., Eich, R., Shibayama, N., Olson, J.S., and Morimoto, H. "Rate Constants for O2 and CO Binding to the a and b Subunits within the R and T States of Human Hemoglobin." J. Biol. Chem., 273 (1998) : 23150-23159.

     
     Book chapters
     

    Thom, C. S., Dickson, C. F., Olson, J. S., Gell, D. A., and Weiss, M. J. "Normal and Abnormal Hemoglobins." Chapter 19, NATHAN and OSKI's HEMATOLOGY OF INFANCY AND CHILDHOOD 8th Edition (January 3, 2015)

     
     

    Hastings, J. W. and Olson, J. S. "Quentin H. Gibson 1918-2011." National Academy of Sciences Biographical Memoirs, (2014) : 41 pages. http://www.nasonline.org/member-directory/biographical-memoirs.html Free Access.

     
     

    Olson, J.S. and Gutfreund, H. "Quentin H. Gibson: December 9, 1918 – March 16, 2011." Biographical Memoirs of Fellows of the Royal Society, Royal Society of London, 60 (2014) : 169-210.

     
     

    Culbertson, D. and Olson, J.S. "Folding and assembly of myoglobins and hemoglobins." Protein Folding and Metal Ions: Mechanisms, Biology, and Disease (edited Gomes, C.M. and Wittung-Stafshede, P.), Taylor & Francis Books, Inc., Chapter 6 (2010, October ) : 97-122.

     
     

    Olson, J.S., and Ghosh, A. "Mammalian myoglobin as a model for understanding ligand affinities and discrimination in heme proteins." Chapter 1, The Smallest Biomolecules: Perspectives on Heme-Diatomic Interactions (2007) In Press

     
     

    Olson, J.S., and Maillett, D.H. "Designing recombinant hemoglobin for use as a blood substitute." Chapter 27 Blood Substitutes (2005) : 354-374.

     
     

    Olson, J.S., Foley, E.W., Maillett, D.H., and Paster, E.V. "Measurement of rate constants for the reactions of O2, CO, and NO with hemoglobin." Molecular Medicine Series (Ronald L. Nagel, editor), 82 (2003) : 65-91.

     
     Other
     

    Olson, J.S. "Novel Hemoglobin Mutants Having Reduced Nitric Oxide Binding." U.S. Patent Application (Provisional), No.: 360/US

     
     

    Olson, J.S., Aitken, J.F., and Mathews, A.J. "Mutant Hemoglobins Containing Heme Pocket Mutations." U.S. Patent Application Serial No. 08/381, 175, Docket #220

     
     

    Olson, J.S., Whitaker, T.L., and Hargrove, M.S. "Hemoglobin Mutants that Reduce Heme Loss." U.S. Patent Application Serial No. 60/006,020, Docket #251

     
    Presentations
     Invited Talks
     

    "Ligand discrimination by hemoglobins and myoglobins: How does it work and why." Symposium on Frontiers of Iron Chemistry in Biology, Pacifichem 2015 Meeting (ACS sponsored), Honolulu, HI . (December 2015)

     
     

    "Strategies for Engineering rHBOCs: Compromises between Hemoglobin Stability, Production, Function, and Toxicity In Vivo." International Symposium on Blood Substitutes (ISBS –Toward Novel Blood Transfusion Therapies), Lund, Sweden. (June 2015)

     
     

    "An Introduction to Selectively Engineering Ligand Affinities and Resistance to Autooxidation in Heme Proteins." XVIIIth International Conference on Oxygen Binding and Sensing Proteins (O2BiP), University of Sheffield. (July 2014)

     
     

    "The Kinetics of Ligand Binding: From Pac-Man to Pathways." Symposium Honoring Florante A. Quiocho, BCM Biochemistry. (September 2014)

     
     

    "Transfusions, Hypertension, Evolution, and Baseball Gloves: Lessons Learned from 46 Years of Hemoglobin Research." University of New York, NYC. (September 2014)

     
     

    "Hemoglobin Evolution and Engineering: From Baseball Gloves to Blood Substitutes." University of Nebraska, Lincoln, NE. (September, 2013)

     
     

    "Recombinant Hb-Based Oxygen Carriers: Optimizing Design, Testing Toxicities, and Enhancing Production." Sangart External Scientific Advisory Board Meeting , San Diego, CA. (March, 2013)

     
     

    "Recombinant Hb-Based Oxygen Carriers: Optimizing Design, Testing Toxicities, and Enhancing Production." Citywide Transfusion Medicine conference, Methodist Hospital, Houston, TX. (April, 2013)

     
     

    "The Kinetics of Ligand Binding: From Pac-Man to Pathways." Symposium Honoring Florante A. Quiocho, Baylor College of Medicine , Houston, TX. (September, 2013)

     
     

    "“Gates versus Tunnels: from Alkyl Isocyanides to Mutagenesis Mapping” Symposium sponsored by Department of Biochemical Sciences, Sapienza University of Rome, Rome, Italy." (November 2012)

     
     

    "Acellular Hb Toxicity, NO Dioxygenation, and Engineering Hb-Based Oxygen Carriers (HBOCs)." Greater Philadelphia Red Cell Club, Children's Hospital of Philadelphia, PA. (October 2011)

     
     

    "Engineering Recombinant Hb-Based O2 Carriers to Be More Effective, Safer, and Easier To Produce." XIII International Symposium on Blood Substitutes and Oxygen Therapeutics (ISBS) , Boston, MA. (August 2011)

     
     

    "NO Dioxygenation, Globin Evolution, and Recombinant Hb-based Blood Substitutes." Department of Biochemistry & Molecular Biology, University of Kansas Medical School, Kansas City, KS. (April 2011)

     
     

    "Recombinant Hb-Based Oxygen Carriers: Optimizing Design, Testing Toxicities, and Production Problems." Interagency Alternative Oxygen Therapeutics Working Group , NIH/DOD/FDA, Massachusetts General Hospital, Boston, MA. (July 2011)

     
     

    "Strategies for Engineering Safer, More Efficient, and More Stable Recombinant Hemoglobins for Use as O2 Delivery Pharmaceuticals." Sangart, Inc., San Diego, CA. (October 2011)

     
     

    "Lessons Learned from Mutagenesis: Reactions of NO with Mbs and Hbs." Second Annual Wake Forest Nitrogen Oxide Conference at Nemacolin Resort, Farmington, PA. (May 2008)

     
     

    "Biophysics, Baseball Gloves, and Blood Substitutes." 51st Annual Biophysical Society Meeting, Baltimore, Maryland. (March 2007)

     
     

    "Designing Recombinant Hemoglobin to be a Safe, Effective, and Commercially Feasible Blood Substitute." European Society of Hematology -Club du Globule Rouge et du Fer Euroconference: DISORDERS OF IRON HOMEOSTASIS, ERYTHROCYTES AND ERYTHROPOIESIS, Athens, Greece. (November 2007)

     
     

    "Heme Protein Engineering: Selective Oxygen Filters, Artificial Gills, and Hemoglobin-Based Blood Substitutes." Iowa State Student Micro Symposium, Iowa State University, Ames, Iowa. (March 2007)

     
     

    "Hemoglobin-Based Oxygen Carriers (HBOCs) and Hypertension." Structural Basis of Human Disease -- Baylor College of Medicine Graduate Course, Houston, Texas. (April 2007)

     
     

    "NO Dioxygenation by Single and Multi-domain Globins: The Other Function of Hemoglobins." Southwest Region ACS Meeting, Symposium: Redox Proteins Catalytic and Regulatory Roles (in honor of Dr. David Knaff), Lubbock, Texas. (November 2007)

     
     

    "Pathways for O2 binding to globins, NO dioxygenation, and Hb-based Blood Substitutes." University of Texas Medical Branch, Galveston, Texas. (September 2007)

     
     

    "Designing Recombinant Hemoglobin to Be a Safe and Effective Blood Substitute." 48th American Society of Hematology Annual Meeting and Exposition, Orlando, Florida. (December 2006)

     
     

    "O2 Filters, Artificial Gills, and Hemoglobin-Based Blood Substitutes: Mimicking Nature by Protein Engineering." IVth International Conference on Dioxygen Binding and Sensing Proteins, Stazione Zoologica Anton Dohrn, Napoli, Italy. (September 2006)

     
     

    "Blood Pressure, NO Dioxygenation, Hemoglobin Expression, and the Design of Blood Substitutes." Iowa State University, Ames, Iowa. (September 16-17, 2004)

     
     

    "Electrostatics, Baseball Gloves, Worms, and Fungi: Lessons on Hemoglobin Physiology." Austen Riggs - Adventures in Hemoglobin Research, University of Texas, Austin, Texas. (November 12-14, 2004)

     
     

    "The Design of Blood Substitutes: From Baseball Gloves to Brewing Hemoglobin." Carnegie Mellon University and Pittsburgh Life Sciences Greenhouse, Pittsburgh, Pennsylvania. (December 8-9, 2004)

     
     

    "Blood Pressure, Baseball Gloves, and Blood Substitutes: Using Molecular Biophysics to Engineer Heme Proteins." Department of Chemistry, University of Texas, San Antonio, San Antonio, Texas. (September 8, 2003)

     
     

    "Blood, Toil, Tears, and Sweat: Using Molecular Biophysics to Design O2 Delivery Pharmaceuticals." Department of Chemistry, University of Texas, San Antonio, San Antonio, Texas. (September 8, 200)

     
     

    "Blood, Toil, Tears, and Sweat: Using Molecular Biophysics to Design O2 Delivery Pharmaceuticals." Department of Chemistry, University of Texas, El Paso, El Paso, Texas. (March 28, 2003)

     
     

    "Electrostatics, Baseball Gloves, and Blood Substitutes." Department of Biochemistry & Cell Biology, Rice University, Houston, Texas. (March 31, 2003)

     
     

    "NO Dioxygenation and the Hypertensive Effect of Hb-based Blood Substitutes." 10th Annual Meeting of the Society for Free Radical Biology and Medicine, Workshop 1, Methods in Free Radical Biology and Medicine and their Application, Seattle, Washington. (November 20, 2003)

     
     

    "Blood, Toil, Tears, and Sweat: The Design of Second Generation O2 Delivery Pharmaceuticals." New England Biolabs, Beverly, Massachusetts. (May 13, 2002)

     
     

    "NO Dioxygenation, Vasoregulation, and blood Substitutes." Dept. of Biochemistry and Molecular Pharmacology, U Mass Med. School, Worcester, Massachusetts. (May 15, 2002)

     
     

    "Protein Engineering at Rice." Special Session at the May Board of Trustees Meeting, Rice University. (Houston, Texas)

     
     

    "Protein Engineering at Rice." Lord Sainsbury, Parliamentary Under Secretary of State for Science, Department of Trade and Industry, U.K.. (June 12, 2002)

     
     

    "Protein Engineering at Rice." Tanox delegation, Rice University. (September 26, 2002)

     
     Lectures
     

    "Blood Substitutes (HBOCs), Transfusions with Old Red Blood Cells, and the Toxicity of Plasma Cell-free Hemoglobin." Structural Basis of Human Disease - Baylor College of Medicine Graduate Course, Houston, Texas. (May 2015)

     
     

    "Blood Substitutes (HBOCs), Transfusions with Old Red Blood Cells, and the Toxicity of Plasma Cell-free Hemoglobin." Structural Basis of Human Disease - Baylor College of Medicine Graduate Course, Houston, Texas. (May 2014)

     
     

    "Blood Substitutes (HBOCs), Transfusions with Old Red Blood Cells, and the Toxicity of Plasma Cell-free Hemoglobin." Structural Basis of Human Disease - Baylor College of Medicine Graduate Course, Houston, TX. (May, 2013)

     
     

    ""Recombinant Hb-Based Oxygen Carriers" BCM Course Structural Basis of Human Disease." (April 2012)

     
     

    "“Recombinant Hb-Based Oxygen Carriers: Optimizing Design, Testing Toxicities, and Production Problems,” – Rice University course, BIOE 615: Bioengineering and Cardiac Surgery.." (November 2012)

     
     

    "Hemoglobn disorders and structure-based design of blood substitutes." Baylor College of Medicine Graduate Course, Houston, TX. (May 2011)

     
     

    "Hemoglobn disorders and structure-based design of blood substitutes." Structural Basis of Human Disease - Baylor College of Medicine Graduate Course, Houston, Texas. (April 2010)

     
     

    "Evolution of O2 transport and storage by hemoglobins: From Bacteria to Plants and Animals." AP Biology Teachers Workshop, Rice University, Houston. (June 2008)

     
     

    "Hemoglobin-Based Oxygen Carriers (HBOCs) and Hypertension." Structural Basis of Human Disease - Baylor College of Medicine Graduate Course, BCM, Houston, TX. (April 2008)

     
     

    "Structure Based Design of Hb-Based Blood Substitutes." Structural Basis of Human Diseases (BCM 310-423J & 311-423J), Baylor College of Medicine, Houston, Texas. (April 3, 2004)

     
     

    "Blood Pressure, Baseball Gloves, and Blood Substitutes." Advances in Tissue Engineering Course, CETE and Department of Bioengineering, Rice University, Houston, Texas. (August 15, 2003)

     
     

    "The Design of Blood Substitutes." Baylor College of Medicine Course, Structural Basis of Human Disease, Texas Medial Center, Houston, Texas. (May 1, 2003)

     
     

    "Blood, Toil, Tears, and Sweat: The Design of Second Generation O2 Delivery Pharmaceuticals." Advances in Tissue Engineering course, CETE and Dept. of Bioengineering, Rice University, Houston, Texas. (August 17, 2002)

     
     

    "Structural Basis of the Design of Blood Substitutes." Baylor College of Medicine Course "Structural Basis of Human Disease", Texas Medical Center, Houston, Texas. (April 25, 2002)

     
     Other
     

    "Alliances For Graduate Education And The Professoriate (Agep) At Rice University." NIGMS Workshop on Achieving Scientific Excellence Through Diversity, Bethesda, Maryland. (May 5-6, 2001)

     
     

    "Blood Substitutes." Invited lecture, Advances in Tissue Engineering Course, CETE and Department of Bioengineering, Rice University, Houston, Texas. (August 15, 2001)

     
     

    "De-mystifying the Reactions of NO With Hemoglobins and Myoglobins." invited speaker for Symposium on Interplay between Nitric Oxide and Hemoglobin: Current Concepts, The American Physiology Society Meeting, Orlando, Florida. (April 1, 2001)

     
     

    "NO Dioxygenation, Vasoregulation, and Blood Substitutes." invited lecture, Department of Biochemistry, University of Wisconsin, Madision, Wisconsin. (April 9, 2001)

     
     

    "O2 Delivery Pharmaceuticals, NO Scavenging, and Vasoregulation." Guest lecture for Baylor College of Medicine course entitled "Structural Basis of Human Diseases", Houston, Texas. (April 12, 2001)

     
     

    "Protein Engineering: Culmination of the Revolution in Biology." invited lecture for Continuing Studies Course: Encore: A Selection of Rice Teaching Award Winners, Houston, Texas. (November 26, 2001)

     
     

    "Science vs. Religion: From Evolution to the Human Genome." invited lecture for Continuing Studies Course: This Year's Best: 2001 Rice University Teaching Award Winners, Houston, Texas. (October 4, 2001)

     
     

    "Blood Substitues, An Update." Lecture, Advances in Tissue Engineering Course, Rice University, Houston, Texas. (August 13, 2000)

     
     

    "Blood Substitutes and NO Dioxygenation." Workshop on Myoglobin Biophysics and Physiology, Los Alamos National Laboratory, Los Alamos, New Mexico. (November 28, 2000)

     
     

    "Blood and Money: The Rational Design of Second Generation Blood Substitutes." Speaker, Structural Symposium on Structural Biology, UT Medical Branch at Galveston, Texas. (May 20, 2000)

     
     

    "Blood and Money: Using Chemistry and Biophysics to Design Second Generation O2 Delivery Pharmaceuticals." Welch Foundation Lecture Tour, The University of Texas, Permian Basin, Department of Chemistry, Odessa, Texas. (November 8, 2000)

     
     

    "Blood and Money: Using Chemistry and Biophysics to Design Second Generation O2 Delivery Pharmaceuticals." Welch Foundation Lecture Tour, McMurry University (and Hardin Simmons and Abilene Christian Universities), Department of Chemistry,. (November 9, 2000)

     
     

    "Blood and Money: Using Chemistry and Biophysics to Design Second Generation O2 Delivery Pharmaceuticals." Welch Foundation Lecture Tour, Midwestern State University, Department of Chemistry, Wichita Falls, Texas. (November 10, 2000)

     
     

    "NO Dioxygenation, Vasoregulation, And Blood Substitutes." Symposium in Honor of Reinhold and Ruth Benesch, Rockefeller University, New York, City, NY. (October 30, 2000)

     
     

    "Protein Engineering: Culmination of the Revolution in Biology." Lecture for the continuing education course entitled THE YEAR'S BEST: 2000 RICE UNIVERSITY TEACHING AWARD WINNERS, Rice University, Houston, Texas. (October 5, 2000)

     
     

    "Blood and Money: Development of Safe Oxygen Delivery Pharmaceuticals." Society of Rice University Women, Rice University. (October 25, 1999)

     
     

    "Course in The Rational Design of Second Generation Blood Substitutes." Advances in Tissue Engineering, Rice University. (August 22, 1999)

     
     

    "Molecular Biophysics and Blood Substitutes: Designing Second Generation O2 Delivery Pharmaceuticals." Rice Alumni College, Rice University. (April 17, 19999)

     
     Posters
     

    "CaYHb1, a Flavohemoglobin from Candida albicans." Keck Center/HAMBP Annual Research Conference, Houston, Texas. (October 8, 2005)

     
     

    "Myoglobin Binding Pocket Size and Ligand Paths Revealed by Infrared Spectra of Bound Alkyl Isocyanides." AAAS Annual Meeting, Washington, D.C.. (February 13-18, 2005) With G. Blouin

     
     

    "NO Dioxygenation in a Flavohemoglobin from Candida albicans." AAAS Annual Meeting, Washington, D.C. (February 13-18, 2005)

     
     

    "Understanding and Engineering the NO Dioxygenation Mechanism in Hemoglobin and Myoglobin." Gordon Research Conference on Nitric Oxide, Il Ciocco, Italy. (May 22-27, 2005)

     
     

    "Understanding and Engineering the NO Dioxygenation Mechanism in Hemoglobin and Myoglobin." Society for Free Radical Biology and Medicine, Austin, Texas. (November 16-20, 2005)

     
     

    "Understanding and Engineering the NO Dioxygenation Mechanism in Hemoglobin and Myoglobin." Meeting on the Role of Nitrite in Physiology, Pathophysiology, and Therapeutics, NIH, Bethesda, Maryland. (September 8-9, 2005)

     
     Seminar Speaker
     

    "Blood Substitutes, Baseball Gloves, NO Dioxygenation, and Hemoglobin Engineering." Albert Einstein College of Medicine, Yeshiva University, New York City, NY. (March, 2008)

     
     

    "Recombinant Hemoglobin is the Ultimate Starting Material for all Hb-based blood substitutes." NSF REU Cellular Engineering Lunch Seminar, Rice University, Houston. (June 008)

     
     

    "The Development of rHb-Based Oxygen Carriers, NO Scavenging, Hypertension, and Nitrite Reduction." Seminar for Atherosclerosis and Vascular Biology Training Program, Baylor College of Medicine, Houston, TX. (June 2008)

     
     

    "Design of Hemoglobin-Based Oxygen Carriers (HBOCs)." Structural Basis of Human Disease -- Baylor College of Medicine Graduate Course, Houston, Texas. (April 2006)

     
     

    "Design of Third Generation Hemoglobin-Based Oxygen Carriers (HBOCs)." Short Course in Tissue Engineering, CETE, Rice University, Houston, Texas. (August 2006)

     
     

    "O2 Transport, NO Dioxygenation, and the Design of Recombinant Hemoglobin-Based Blood Substitute." Auburn University, Auburn, Alabama. (March 2006)

     
     

    "Design of Hemoglobin-Based Blood Substitutes: Recombinant Hb as a Potential Oxygen Carrier." Short Course in Tissue Engineering, Rice University, Houston, Texas. (August 13, 2005)

     
     

    "Recombinant Hemoglobin: A Potential Starting Material for all Hb-based Blood Substitutes." BioPure, Inc., Cambridge, Massachusetts. (June 10, 2005)

     
     Session Chair
     

    "“How ligands bind to Globins: A Sliding Rule for Affinity, NOD, and Gates versus Tunnels for Entry and Exit” Session Chair, XVIIth International Conference on Oxygen Binding and Sensing Proteins (O2BiP), University of Parma, Parma, Italy." ((August 2012))

     
     

    "NO Binding, NO dioxygenation, and Nitrite Reactions." XVth International Conference on Oxygen Binding and Sensing Proteins (O2BiP), Aarhus University, Aarhus, Denmark. (August 2008)

     
     Workshops
     

    "Strategies for Engineering Safer, More Efficient and More Stable Recombinant Hemoglobins for Use as O2 Delivery Pharmaceuticals." Hemoglobin Based Oxygen Carriers: Current Status and Future Directions, Center for Biologics Evaluation and Research, FDA, The National Heart, Lung, and Blood Institute, NIH and Office of the Secretary and Office of Public Health and Science, DHHS, NIH Campus, Bethesda, Maryland. (April 2008)

     
    Editorial Positions
     Member of the Editorial Board, Journal of Biological Chemistry. ASBMB. (2010 - 2015)

    Supervised Theses & Dissertations
     Ph.D. The Molecular Bases of Tryptophan Replacement Effects in RecA: A Biochemical and Biophysical Characterization of Single- and Null-Tryptophan Mutant Escherichia coli RecA Protein. (2002) (Committee Member)

     Ph.D. Polyelectrolyte and Hydrophobic Effects in Protein-DNA Interactions: PurR, LacI, and Human p53 Proteins. (2002) (Committee Member)

     Ph.D. Key Enzymes in Parasite Sterol Metabolism. (2002) (Committee Member)

     Ph.D. Repressor Proteins: Ligand Binding, Thermodynamics and Assembly. (2002) (Committee Member)

     Ph.D. The Effects of Amino Acid Substitution on Apomyoglobin Stability, Folding Intermediates, and Holoprotein Expression. (2002) (Thesis or Dissertation Director)

     Miguel L. Teodoro, Ph.D. Modeling Protein Flexibility Using Collective Modes of Motion: Applications to Drug Design. (2003) (Committee Member)

     David H. Maillett, Ph.D. Engineering Hemoglobins and Myoglobins for Efficient O2 Transport. (2003) (Thesis or Dissertation Director)

     Rachel Leininger Schweers, Ph.D. Electrostatic Regulation of O2 and CO Binding in the a and b Subunits of Recombinant Human Hemoglobin. (2003) (Thesis or Dissertation Director)

     John Bruning, Ph.D. Structural and Thermodynamic Analysis of Human PCNA Bound to Peptides Derived from DNA Polymerase-delta p66-Subunit and Flap Endonuclease-1 (FEN1) Proteins. (2004) (Committee Member)

     Angela Hale, M.A. Electrostatic Regulation of Oxygen Binding to the Neuronal Hemoglobin of Cerebratulus lacteu. (2004) (Thesis or Dissertation Director)

     Jeffery C. Myers, Ph.D. Human UP1 as a Model for Understanding Purine Recognition in the Family of Proteins Containing the RNA Recognition Motif (RRM). (2004) (Committee Member)

     Erin Foley, Ph.D. Physiologically Relevant Reactions of Myoglobin and Hemoglobin with NO. (2005) (Thesis or Dissertation Director)

     Jamie Catanese, Ph.D. Biophysical and Biochemical Characterization of the Disconnected Interacting Protein 1, DIP1, from Drosophila melanogaster. (2005) (Committee Member)

     Michelle K. Calabretta, Ph.D. Biophysical Characterization of Gold Nanocrystal Protein Conjugates: Formation, Stoichiometry and Function. (2005) (Committee Member)

     Renee LeClair, Ph.D. Elucidating the Mechanism of Triterpene Cyclization Using DNA Synthesis and Phylogenetic Approaches. (2005) (Committee Member)

     Hongli Zhan, Ph.D. Biophysical Characterization of the Allosteric Transition in Lactose Repressor Protein (LacI). (2005) (Committee Member)

     Rafael Counago, Ph.D. Single Gene Evolution: A Punctuated History of Chance. (2005) (Committee Member)

     Angela Noel Hvitved, Ph.D. NO Dioxygenation in Mammalian Hemoglobins and Microbial Flavohemoglobins. (2007) (Thesis or Dissertation Director)

     Adina Maximiuc, Ph.D. Structural Basis for Calmodulin-mediated Regulation of the Ryanodine Receptor. (2008) (Committee Member)

     Megan Guelker, Ph.D. Overcoming the Challenges of Twinning and Pseudo Symmetry in Solving the Structure of Flavodoxin from Desulfovibrio desulfuricans 29577 by X-ray Crystallography. (2008) (Committee Member)

     Ian Dews, Ph.D. The Four Syndecans: A Fugue in GxxxG Minor. (2008) (Committee Member)

     Todd Jaszewski, Ph.D. Specificity of Transmembrane Domain Self-association by Mutagenesis and Protein Design. (2008) (Committee Member)

     Crystal Stanworth, M.A. The Saccharomyces cerevisiae Hsp70 Escort Protein, Hep1, Exhibits Broad-spectrum Chaperone Activity. (2008) (Committee Member)

     George Blouin, Ph.D. Alkyl Isocyanides as Transition State Analogs for Ligand Entry and Exit in Globins. (2008) (Thesis or Dissertation Director)

     Aaron Collier, Ph.D. Initiation of RNA polymerization and polymerase encapsidation by a Picobirnavirus. (2014) (Committee Member)

     Truong Nguyen, Ph.D. Understanding dynamic activities of genetic networks under environmental changes . (2015) (Committee Member)

     Anisha Perez, Ph.D. Predicting the Biochemical Parameters of an Antibiotic Efflux Pump Using a Mathematical Model. (2016) (Committee Member)

     Lu Han, PhD Method development for natural products structure determination. (Committee Member)

     Xu Wang, PhD Structure-function studies of E. faecalis YybT and GshF associated with daptomycin resistance. (Committee Member)

     Premila P. Samuels Mohan Das, PhD Engineering recombinant myoglobins and hemoglobins for optimized globin stability. (Co-Director)

     Yanlin Fan, PhD Structure and function studies of Orsay virus. (Committee Member)

    Awards, Prizes, & Fellowships
     Fellow, American Association for the Advancement of Science,

     Attendee, Robert A. Welch Foundation Meeting, "Chemistry for the 21st Century," Houston, Texas, (October 29-30, 2001)

     Member, American Chemical Society,

     Member, Biophysical Society,

     Member, American Society of Biological Chemists,

     Attendee, American Physiological Society Meeting (FASEB), Orlando, Florida, (March 31-April 2, 2001)

     Fellow, American Association for the Advancement of Science

     Member, American Society of Biological Chemists

     Attendee, Structural Symposium on Structural Biology 2000, The University of Texas Medical Branch, Galveston, Texas (May 19-21, 2000)

     Attendee, Keck Symposium 2000, Structural and Functional Genomics, Rice University and Baylor College of Medicine, Houston, Texas (October 16-17, 2000)

     Member, Biophysical Society

     Attendee, Symposium in Honor of Reinhold and Ruth Benesch, Rockefeller University, New York City, New York (October 30, 2000)

     Attendee, Workshop on Myoglobin Biophysics and Physiology, Los Alamos National Laboratory, Los Alamos, New Mexico (November 28-29, 2000)

     Attendee, Welch Foundation 44th Conference on Chemical Research, "Macromolecular Structures and Function," Houston, Texas (October 23-24, 2000)

     Member, American Chemical Society

     Attendee, NSF Summit Meeting on Minorities in Sciences and Engineering, Rice University (October 18-19, 1999)

     Attendee, Robert A. Welch Foundation Meeting "Synthetic and Biological Chemistry," Houston, Texas (October 25-26, 1999)

     Attendee, Scientific Advisory Board Meeting for Baxter Health Care Corporation, Boulder, Colorado (March 22-24, 1999)

    Positions Held
     Adjunct Professor of Pediatrics-Hematology & Oncology, Baylor College of Medicine. (2014 - 2014)