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Scholarly Interest Report
         
Daniel S. Wagner
Associate Professor
Associate Professor in Biochemistry and Cell Biology
 
e-mail:dswagner@rice.edu
 
  • B.A. Biochemistry (1990) University of Texas, Austin, Texas
  • Ph.D. Genes and Development Program (1997) University of Texas Health Science Center, Houston, Texas
 
Primary Department
   Department of BioSciences
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Department Affiliations
 
  • Institute of Biosciences and Bioengineering
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    Research Areas
     Analysis of development of the zebrafish embryo to understand the genetic basis of cell differentiation and cell behavior. Our work examines the genetic regulation of diverse developmental events including epidermal differentiation, angiogenesis and post transcriptional regulation in the teleost yolk cell. This work is directed at understanding essential processes that are misregulated in human disease as well as the evolutionary plasticity that drive diversity.
     

    Research Statement 2009

     

    My laboratory investigates the function of essential elements required to execute vertebrate morphogenetic programs. Morphogenesis is a collective term for the processes by which cells move and change shape to transform the relatively featureless blastoderm into a recognizable vertebrate embryo. While the mechanisms of embryonic patterning have been investigated in some detail, morphogenesis remains poorly understood. To investigate vertebrate morphogenetic movements we analyze early development of the zebrafish, Danio rerio. The zebrafish embryo provides an excellent model for studying morphogenesis since they are optically transparent, develop rapidly and mutants have been identified that display defective morphogenetic movements.Wagner's Research In previous work I identified several mutants that are defective in epiboly movements and one mutant defective in anterior morphogenesis. We are now using these mutants to dissect morphogenetic movements. We are applying genetic, molecular and embryological approaches to determine the precise nature of the mutations and the morphogenetic defects we observe. Our immediate goals are to identify the genes encoded by the mutant loci and to determine in detail the defects that result from these mutations. By coupling the molecular identity of the mutants with their specific defects we will gain deep insight into the mechanisms of vertebrate morphogenesis.

     
    Teaching Areas
     BIOC447 Experimental Biology and the Future of Medicine

    BIOC547 Experimental Biology and the Future of Medicine

    HONS 470 Rice Undergraduate Scholars Program

    HONS 471 Rice Undergraduate Scholars program

    BIOC310 Undergraduate Research
     
    Selected Publications
     Refereed articles
     

    D.P. Noren, W.H. Chou, S.H. Lee, A.S. Popel, A.A. Qutub, A. Warmflash, D.S. Wagner, A. Levchenko. VEGF-Mediated Ca2+ Signaling Steers Endothelial Cell Phenotypes by a Combination of Stochastic and Deterministic Decoding.  Science Signaling  (In Press)

     
     
    E.C. Swindell, Q. Yuan, L.E. Maili, B. Tandon, D.S. Wagner, J.T. Hecht 2015 Crispld2 is required for neural crest cell migration and cell viability during zebrafish craniofacial development. Genesis 53 (10), 660-667
     
     
     

    G.T. Eisenhoffer, G. Slattum, H. Otsuna, C.D. Bryan, J. Lopez, D.S. Wagner, C-B. Chien, R.I. Dorksy, J Rosenblatt. A cell and molecular toolbox to study epithelia in zebrafish submitted to Journal of Cell Science

     
     

    S Hirota, T.P. Clements, L.K. Tang, J.E. Morales, H.S. Lee, S.P. Oh, G.M. Rivera, D.S. Wagner, J.H. McCarty 2015 Neuropilin-1 balances β8 integrin-activated TGFβ signaling to control sprouting angiogenesis in the brain. Development 142(24):4363-73. PMCID: PMC4689212

     
     

    D.P. Noren, W.H. Chou, S.H. Lee, A.S. Popel, A.A. Qutub, A. Warmflash, D.S. Wagner, A. Levchenko. VEGF-Mediated Ca2+ Signaling Steers Endothelial Cell Phenotypes by a Combination of Stochastic and Deterministic Decoding.  Science Signaling  (In revision)

     
     
    S Hirota, T.P. Clements, L.K. Tang, J.E. Morales, H.S. Lee, S.P. Oh, G.M. Rivera, D.S. Wagner, J.H. McCarty 2015 Neuropilin-1 balances β8 integrin-activated TGFβ signaling to control sprouting angiogenesis in the brain. Development 142(24):4363-73. PMCID: PMC4689212 
     
     
     

    G. de la Garza, J.R. Schleiffarth, M. Dunnwald, A. Mankad, J. L. Weirather, G.Bonde, S. Butcher, T.A. Mansour, Y. Koussa, C. F. Fukazawa, , D.W. Houston, J. Manak, , B. C Schutte, D.S. Wagner, R.A. Cornell "Interferon Regulatory Factor 6 Promotes Differentiation Of The Periderm By Activation Of grainyhead-like 3." Journal of Investigative Dermatology, 133 (2013) : 68-77.

     
     

    Masiello CA, Chen Y, Gao X, Liu S, Cheng HY, Bennett MR, Rudgers JA, Wagner DS, Zygourakis K, Silberg JJ "Biochar and microbial signaling: production conditions determine effects on microbial communication." Environ Sci Technol (2013) : es401458s.

     
     

    Lukianova-Hleb EY, Ren X, Constantinou PE, Danysh BP, Shenefelt DL, Carson DD, Farach-Carson MC, Kulchitsky VA, Wu X, Wagner DS, Lapotko DO "Improved Cellular Specificity Of Plasmonic Nanobubbles Versus Nanoparticles In Heterogeneous Cell Systems." PLoS One , 7(4) (2012) : e34537.

     
     

    Lukianova-Hleb EY, Wagner DS, Brenner MK, Lapotko DO. "Cell-specific transmembrane injection of molecular cargo with gold nanoparticle-generated transient plasmonic nanobubbles. ." Biomaterials , 33(21) (2012) : 5441-50.

     
     

    de la Garza G, Schleiffarth JR, Dunnwald M, Mankad A, Weirather JL, Bonde G, Butcher S, Mansour TA, Kousa YA, Fukazawa CF, Houston DW, Robert Manak J, Schutte BC, Wagner DS, Cornell RA. "Interferon Regulatory Factor 6 Promotes Differentiation of the Periderm by Activating Expression of Grainyhead-Like 3 ." J Invest Dermatol. (2012 Nov 29 [Epub ahead of print] ) In Press

     
     

    Lukianova-Hleb EY, Huye LE, Wagner DS, Brenner MK, Lapotko DO "Guided Intracellular Injection Of Molecular Cargo With Plasmonic Nanobubble Flow System." Journal of Controled ReleaseIn Revision

     
     

    Lukianova-Hleb EY, Oginsky AO, Samaniego AP, Shenefelt DL, Wagner DS, Hafner JH, Farach-Carson MC, Lapotko DO Tunable Plasmonic Nanoprobes for Theranostics of Prostate Cancer. Theranostics. 2011;1(1):1-15.

     
     

    Miller R, Gomez de la Torre Canny S, Jang CW, Cho KH, Wagner DS, Jones E, Habas R, McCrea P "Daam1 Mediated PCP Signaling Regulates Pronephric Kidney Tubule Morphogenesis." of the American Society of Nephology, 9 (2011) : 1654-64.

     
     

    Fukazawa C, Santiago C, Park KM, Deery WJ, Gomez de la Torre Canny S, Holterhoff CK, Wagner DS. poky/chuk/ikk1 is required for differentiation of the zebrafish embryonic epidermis. Dev Biol. 2010 Oct 15;346(2):272-83

     
     

    Lukianova-Hleb, E., Santiago, C., Wagner, D.S., Hafner, J., and Lapotko, D. "Generation and detection of plasmonic nanobubbles in zebrafish." Nanotechnology, 21 (2010) : 225102.

     
     

    Veth KN, Willer JR, Collery RF, Gray, MP, Willer GB, Wagner DS, Mullins MC, Udvadia AJ, Smith RS, John, SWM, Gregg RG, Link BA Mutations in zebrafish lrp2 result in adult onset ocular pathogenesis that models myopia and other risk factors for glaucoma. 2011 PLoS Genetics

     
     

    Wagner DS, Delk NA, Lukianova-Hleb EY, Hafner JH, Farach-Carson MC, Lapotko DO. The in vivo performance of plasmonic nanobubbles as cell theranostic agents in zebrafish hosting prostate cancer xenografts. Biomaterials. 2010 Oct;31(29):7567-7574.

     
     

    Holloway, B.A., Gomez de la Torre Canny, S., Ye, Y., Slusarski, D.C., Freisinger, C.M., Dosch, R., Chou, M.M., Wagner, D.S., and Mullins, M.C. "A novel role for MAPKAPK2 in morphogenesis during zebrafish development." PLoS Genetics, 5 (2009) : e1000413.

     
     

    Holterhoff, C.K., Saunders, R.H., Brito, E.E., and Wagner, D.S. "Sequence and expression of the zebrafish alpha-actinin gene family reveals conservation and diversification among vertebrates." Developmental Dynamics, 238 (2009) : 2936-2947.

     
     

    Birely, J., Schneider, V.A., Santana, E., Dosch, R., Wagner, D.S., Mullins, M.C., and Granato, M. "Genetic screens for genes controlling motor nerve-muscle development and interactions." Developmental Biology, 280 (2005) : 162-176.

     
     

    Panzer, J.A., Gibbs, S.M., Dosch, R., Wagner, D., Mullins, M.C., Granato, M., and Balice-Gordon, R.J. "Neuromuscular synaptogenesis in wild-type and mutant zebrafish." Developmental Biology, 285 (2005) : 340-357.

     
     

    Conejo-Garcia, J.R, Benencia, F., Courreges, M.-C., Kang, E., Mohamed-Hadley, A., Buckanovich, R.J, Holtz, D.O, Jenkins, A., Na, H., Zhang, L., Wagner, D.S, Katsaros, D., Caroll, R., and Coukos, G. "Tumor-infiltrating dendritic cell precursors recruited by a beta-defensin contribute to vasculogenesis under the influence of Vegf-A." Nature Medicine, 10 (2004) : 950-958.

     
     

    Dosch, R., Wagner, D.S., Mintzer, K.A., Runke, G., Wiemelt, A.P., and Mullins, M.C. "Maternal control of vertebrate development before the midblastula transition: Mutants from the zebrafish I." Developmental Cell, 6 (2004) : 771-780.

     
     

    Wagner, D.S., Dosch, R., Mintzer, K.A., Wiemelt, A.P., and Mullins, M.C. "Maternal control of vertebrate development at the midblastula transition and beyond: Mutants from the zebrafish II." Developmental Cell, 6 (2004) : 781-790.

     
    Presentations
     Invited Talks
     

    "Kinase signaling pathways regulating zebrafish morphogenesis." University of Toronto, Canada. (November 6, 2009)

     
     

    "Kinase signaling pathways regulating zebrafish morphogenesis." Louisiana State University Health Science Center, New Orleans. (April 13, 2009)

     
     

    "Push me pull you: Signaling pathways regulating zebrafish morphogenesis." Developmental Biology Program, Vanderbilt University, Nashville, TN. (February 12, 2009)

     
     

    "Push me pull you: Signaling pathways regulating zebrafish morphogenesis." University of Texas, Austin. (March 3, 2009)

     
     

    "Push me Pull you: Signaling pathways regulating zebrafish morphogenesis ." Trinity University Biology Department, San Antonio, Texas. (November 3, 2008)

     
     

    Faculty mentor.  "p38 MAPK signaling is required for zebrafish epiboly." Southwest and Gulf Coast Regional Society for Developmental Biology Meeting, Houston TX. (March 28-30, 2008)

     
     

    "Instructions from Mom: Maternal Control of Zebrafish Development." Program in Genetics, Texas A&M University, College Station, Texas. (February 2, 2006)

     
     

    "Instructions from Mom: Maternal Control of Zebrafish." Research Seminar Series in Cancer & Developmental Biology, University of Kansas Medical School. (September 8, 2005)

     
     

    "From the Egg to the Embryo: Maternal Control of Zebrafish Development." Department of Biochemistry & Cell Biology, Rice University, Houston, Texas. (January 21, 2003)

     
     

    "Maternal Genes Required for Embryonic Morphogenesis." Texas Regional Zebrafish Meeting, College Station, Texas.

     
     Lectures
     

    "pollywog: an egg-cyting yolk tale" Southwest Regional SDB Meeting, Austin TX Oct1-3, 2010 (with Christopher Holterhoff)

     
     

    poky/chuk/ikk1 Is Required For Differentiation Of The Zebrafish Embryonic Epidermis. 9th International Zebrafish Meeting on Development and Genetics, Madison WI June 16-20 2010

     

     
     Posters
     

    "Homologous Recombination (HR) based genome editing using the CRISPR-Cas mechanism in zebrafish." 11th Internation Conference on Zebrafish Genetics and Development, University of Wisconsin, Madison. (June 24-28, 2014) With Clements TP, Abu-Shamat F, Rothfeld J, Chou W1

     
     

    "Identifying Regulatory Elements In 3’-UTR of mRNAs." Society for Developmental Biology 73rd Annual Meeting , Univ. of Washington, Seattle, WA. (July 17-21, 2014 ) With Tandon B, Gomez de la Torre Canny S, Nnoromele C, Jensen K

     
     

    "The Role Of p38 MAPK/MAPKAPK2 Signaling In The Regulation Of Contractility In The Yolk Cell During Epiboly In Zebrafish Embryos" 9th International Zebrafish Meeting on Development and Genetics, Madison WI June 16-20 2010 (with Sol Gomez de la Torre Canny)

     
     

    "The Role of aggf1 in Zebrafish Angiogenesis" Southwest Regional SDB Meeting, Austin TX Oct1-3, 2010 (With Navonil Ghosh)

     

     
     

    "Go Fish! Developmental stages in zebrafish." Genesis, (2009) With S. Gomez de la Torre Canny, C. Holterhoff, R. Behringer, D.J. Kozlowski

     
     

    advisor.  "MAP kinase signaling in early zebrafish morphogenesis." Rice Undergraduate Research Symposium, Rice University. (2009) With K.M. Park

     
     

    advisor.  "The role of MAP kinase kinases in zebrafish embryonic development." Rice Undergraduate Research Symposium, Rice University. (2009) With N. Ghosh, C. Santiago, Y.C. Liu, M. Bailey, G. Cody, J. Faust

     
     

    advisor.  "The role of integrin beta1 in zebrafish morphogenesis." Rice Undergraduate Research Symposium, Rice University. (2009) With S. Bartlett

     
     

    "The zebrafish maternal effect mutant poky: when cells proliferate and fail to differentiate." 5th Biennial Texas Zebrafish Conference, Denton, TX. (June 5-7, 2009) With C. Santiago, C. Fennimore-Fukazawa, S. Gomez de la Torre Canny

     
     

    Faculty mentor.  "p38 MAPK signaling is required for zebrafish epiboly." 8th International Conference in Zebrafish Development and Genetics, Madison, Wisconsin. (June 25-29, 2008)

     
     

    Faculty mentor.  "pollywog, a maternal factor necessary for zebrafish morphogenesis." 8th International Conference in Zebrafish Development and Genetics, Madison, Wisconsin. (June 25-29, 2008)

     
     

    Faculty mentor.  "pollywog, a maternal factor necessary for zebrafish morphogenesis." Southwest and Gulf Coast Regional Society for Developmental Biology Meeting, Houston, Texas. (March 28-30, 2008)

     
     

    "The Maternal-Effect Mutant poky Displays Defective Epiboly Progression." Strategic Conference of Zebrafish Investigators, Mount Desert Island Biological Laboratory, Bar Harbor, Maine. (September 15-17, 2005)

     
     Seminar Speaker
     

    "Instructions from Mom: Maternal Control of Zebrafish Development." University of Texas Houston Medical School, Department of Ophthalmology, Houston, Texas. (March 7, 2007)

     
     

    "Instructions from Mom: Maternal Control of Zebrafish Development." MD Anderson Cancer Center, Department of Molecular Genetics, Houston, Texas. (March 12, 2007)

     
     Workshops
     

    ""The Zebrafish Enveloping Layer: A Model of Epidermal Differentiation"." 10th International Conference Zebrafish Development and Genetics. Epiboly: Molecules, Mechanisms and Meanings Workshop (Co-Organizer), Madison, WI. (June 20-24)

     
    Supervised Theses & Dissertations
     Yitai Tang, Ph.D. Characterization of CnrN-mediated Size Regulation in Dictyostelium. (2007) (Thesis Committee Member)

     Diana Dugas, Ph.D. Genetic Analysis of MicroRNA Function in Arabidopsis Development. (2007) (Thesis Committee Member)

     Damian Dalle Nogare, Ph.D. Molecular-Genetic Analysis of Cell Cycle Diversification in Early Zebrafish Embryos. (2007) (Thesis Committee Member)

     Elizabeth Poggio, M.A. Molecular Genetic Analyses of Plant Peroxisome Functions. (2008) (Thesis Committee Member)

     Eric Howlett, PhD A P13-kinase mediated negative feedback regulates neuronal excitability at the Drosophila neuromuscular junctio. (2009) (Thesis Committee Member)

     Wade Kothmann, PhD Signaling mechanisms that control gap junctional coupling between retinal neurons. (2010) (Thesis Committee Member)

     Stephanie Nemir, PhD Harnessing Cell Response to Substrate Rigidity for Tissue Engineering Applicaitons. (2010) (Thesis Committee Member)

     Shivas Amin, PhD Investigation of Mechanisms Regulating Proliferation in the Zebrafish Developmental Program. (2010) (Thesis Committee Member)

     Gregory Cody, MA Investigation of the role of Wee2 in the zebrafish midblastula transition. (2010) (Thesis Committee Member)

     Sol Gomez de la Torre Canny, Ph.D. The role of p38 MAPK/MAPKAPK2 signaling in zebrafish epiboly: using a conserved signaling pathway in the morphogenesis a novel embryonic structure. (2014) (Thesis Director)

     Curtis Warren, Ph.D. Evolution of the Perlecan/HSPG2 gene and Regulation of its Expression by Inflammatory Cytokines in Normal Tissue Models and Cancer. (2014) (Thesis Committee Member)

     James Summerville, Ph.D. Defended Thesis. (2015) (Thesis Committee Member)

     Brian Engel, Ph.D. Defended Thesis. (2015) (Thesis Committee Member)

     Emily Thomas, Ph.D. Graduate Research Advisor. (Thesis Director)

     Mariane Martinez, Ph.D. Graduate Research Advisor. (Thesis Director)

     Ruda Cui, Ph.D. Graduate Research Advisor. (Thesis Committee Member)

     Thomas Clements, Ph.D. Thesis Advisor. (Thesis Director)

     Bhavna Tandon, Ph.D. Thesis Advisor. (Thesis Director)

     Yiwen Liu, Ph.D. Graduate Research Advisor. (Thesis Committee Member)

     Yanwan Dai, Ph.D. Graduate Research Advisor. (Thesis Committee Member)

     Micaela Morgado, Ph.D. Graduate Research Advisor. (Thesis Committee Member)

     Savini Thrikawala, Ph.D. Graduate Research Advisor. (Thesis Committee Member)

     Patricia Chapela, Ph.D. Graduate Research Advisor. (Thesis Director)

     Fanli Zhou, Ph.D. Graduate Research Advisor. (Thesis Committee Member)

    Awards, Prizes, & Fellowships
     Faculty Initiative Fund Award, (2013)