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Scholarly Interest Report
         
George N. Bennett
Professor
E. Dell Butcher Professor of Biochemistry and Cell Biology
 
e-mail:gbennett@rice.edu
 
  • B.S., University of Nebraska, Lincoln, 1968 (Chemistry)
  • Ph.D., Purdue University, West Lafayette, Indiana, 1974 (Biological Sciences)
 
Primary Department
   Department of BioSciences
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Department Affiliations
 
  • Department of Chemical and Biomolecular Engineering
  • Institute of Biosciences and Bioengineering
  •  
     
    Research Areas
     Molecular biology of prokaryotes, metabolic engineering
     
    Research Statement
     

    The laboratory focuses on genetic engineering of metabolic pathways of microbes for production of biofuels and chemicals. bennettwork In order to construct an effective biocatalyst and carry out effective modification of the metabolic pattern of cells, knowledge of the native regulatory processes, enzymes and pathways of the microbe must be understood. We study the responses of bacteria to stresses either encountered in nature or in an industrial fermentor, such as pH, oxygen limitation or salt concentration. These fundamental studies have developed our approaches to metabolic engineering: cofactor engineering-the modification of the availability of redox factors such as NADH; the “cellular refinery” approach of producing multiple compatible products during a process; and the modeling and use of available genetic resources from the large genomic and biochemical databases for optimal metabolic performance.


    Other areas of research involve Clostridium acetobutylicum where we seek to understand and manipulate genes related to butanol production. Efforts are also under way to study the genetic and biochemical basis of biodegradation of nitroaromatic and other hazardous compounds by this soil bacterium. Development of novel DNA technology for assembling gene constructs and introducing multiple chromosomal changes to enhance microbial genetic engineering via “synthetic biology” is also an ongoing project.

     
    Selected Publications
     Abstracts
     

    Zhao, Y., and Bennett, G.N. "The overexpression of cyclopropane fatty acid synthase suppresses solvent production in Clostridium acetobutylicum ATCC 824." Abstracts from American Society for Microbiology 102nd General Meeting, Abstract 0-60 (2002) : 357.

     
     Refereed articles
     

    Mountziaris PM, Shah SR, Lam J, Bennett GN, and Mikos AG. "A rapid, flexible method for incorporating controlled antibiotic release into porous polymethylmethacrylate space maintainers for craniofacial reconstruction." Biomater Sci, epub (2015)


    PMID: 26340063
     
     

    Thakker C, Lin K, Martini-Stoica H1, Bennett GN "Use of transposase and ends of IS608 enables precise and scarless genome modification for modulating gene expression and metabolic engineering applications in Escherichia coli.." Biotechnol J, epub (2015)

     
     

    Thakker C, Martínez I, Li W, San KY, Bennett GN.  Metabolic engineering of carbon and redox flow in the production of small organic acids.  J Ind Microbiol Biotechnol. 42(3):403-422. (2015)


    doi: 10.1007/s10295-014-1560-y
     
     

    Wang D, Thakker C, Liu P, Bennett GN, San KY "Efficient production of free fatty acids from soybean meal carbohydrates." Biotechnol Bioeng, 112: 2324-2333. (2015)

     
     

    Wang D, Wu H, Thakker C, Beyersdorf J, Bennett GN, San KY "Efficient free fatty acid production in engineered Escherichia coli strains using soybean oligosaccharides as feedstock." Biotechnol Prog, 31: 686-694 (2015) 

     
     

    Wu H, Bennett GN, San KY.  Metabolic control of respiratory levels in coenzyme Q biosynthesis-deficient Escherichia coli strains leading to fine-tune aerobic lactate fermentation.  Biotechnol Bioeng.  112(8):1720-1726. (2015)

     
     

    Wu H, Tuli L, Bennett GN, San KY.  Metabolic transistor strategy for controlling electron transfer chain activity in Escherichia coli.  Metab Eng. 28:159-68. (2015)

     
     

    Jang, Y. S., Han, M. J., Lee, J., Im, J. A., Lee, Y. H., Papoutsakis, E. T., Bennett, G., Lee, S. Y. "Proteomic analyses of the phase transition from acidogenesis to solventogenesis using solventogenic and non-solventogenic Clostridium acetobutylicum strains." Appl Microbiol Biotechnol. , 98(11): 5105-5115.  (2014) 


    DOI 10.1007/s00253-014-5738-z
     
     

    Shah, S.R., Henslee, A. M., Spicer, P. P., Yokota, S., Petrichenko, S., Allahabadi, S., Bennett, G. N., Wong, M. E., Kasper, F. K., and Mikos, A. G. "Effects of antibiotic physiochemical properties on their release kinetics from biodegradable polymer microparticles." Pharm. Res., 31(12): 3379-3389.  (2014) 


    doi: 10.1016/j.ymben.2015.01.002
     
     

    Balzer GJ, Thakker C, Bennett GN, San KY. "Metabolic engineering of Escherichia coli to minimize byproduct formate and improving succinate productivity through increasing NADH availability by heterologous expression of NAD+-dependent formate dehydrogenase." Metabolic Engineering, 20 (2013) : 1-8.

     
     

    Cai X, Servinsky M, Kiel J, Sund C, Bennett GN "Analysis of redox responses during TNT transformation by Clostridium acetobutylicum ATCC 824 and mutants exhibiting altered metabolism." Appl Microbiol Biotechnol., 97 (2013) : 4651-4663.

     
     

    Choi, J. D. R., Jang, Y. S., Cho, J. H., Seung, D., Lee, S. Y., Papoutsakis, E. T., Bennett, G. N., and Song, H. "Characterization and evaluation of corn steep liquid in acetone-butanol-ethanol production by Clostridium acetobutylicum." Biotechnol & Bioprocess Engineering, 18 (2013) : 266-271.

     
     

    Jan J, Martinez I, Wang Y, Bennett GN, San KY. "Metabolic engineering and transhydrogenase effects on NADPH availability in Escherichia coli." Biotechnol Prog. (2013) : doi: 10.1002/btpr.1765.

     
     

    Spicer PP, Shah SR, Henslee AM, Watson BM, Kinard LA, Kretlow JD, Bevil K, Kattchee L, Bennett GN, Demian N, Mende K, Murray CK, Jansen JA, Wong ME, Mikos AG, Kasper FK. "Evaluation of antibiotic releasing porous polymethylmethacrylate space maintainers in an infected composite tissue defect model.." Acta Biomater., 9(11) (2013) : 8832-8839.

     
     

    Thakker C, San KY, Bennett GN "Production of succinic acid by engineered E. coli strains using soybean carbohydrates as feedstock under aerobic fermentation conditions." Bioresour Technol., 130C (2013) : 398-405.

     
     

    Wang Y, San KY, Bennett GN. "Cofactor engineering for advancing chemical biotechnology." Curr Opin Biotechnol., 24(6) (2013) : 994-999.

     
     

    Wang Y, San KY, Bennett GN. "Improvement of NADPH bioavailability in Escherichia coli by replacing NAD(+)-dependent glyceraldehyde-3-phosphate dehydrogenase GapA with NADP (+)-dependent GapB from Bacillus subtilis and addition of NAD kinase." J Ind Microbiol Biotechnol., 40(12) (2013) : 1449-1460.

     
     

    Wang Y, San KY, Bennett GN. "Improvement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strains." Appl Microbiol Biotechnol., 97(15) (2013) : 6883-6893.

     
     

    Lee J, Jang YS, Choi SJ, Im JA, Song H, Cho JH, Seung do Y, Papoutsakis ET, Bennett GN, Lee SY "Metabolic engineering of Clostridium acetobutylicum ATCC 824 for isopropanol-butanol-ethanol fermentation." Appl Environ Microbiol., 78(5) (2012 Mar) : 1416-23.

     
     

    Thakker, C., Martinez, I. San, K. Y. and Bennett G. N. "Succinate production in Escherichia coli." Biotechnol J. , 7(2) (2012 Feb) : 213-24.

     
     

    Cai X, Bennett GN "Improving the Clostridium acetobutylicum butanol fermentation by engineering the strain for co-production of riboflavin." J Ind Microbiol Biotechnol, 38 (2011) : 1013-1025.

     
     

    Heath, A. P., Bennett, G. N., Kavraki, L. E. "An algorithm for efficient identification of branched metabolic pathways." Journal of Computational Biology, 18 (2011) : 1575-1597.

     
     

    Heath, A. P., Bennett, G. N., Kavraki, L. E. "Identifying branched metabolic pathways by merging linear metabolic pathways." RECOMB 2011In Press

     
     

    Lee, J.,Jang, Y. S., Song, H., Cho, J. H., Seung, D. Y., Papoutsakis, E. T., Bennett, G. N.,and Lee, S. Y. "Metabolic engineering of Clostridium acetobutylicum ATCC824 for isopropanol-butanol-ethanol fermentation." Applied Environ. Microbiol.In Press

     
     

    Thakker, C., Zhu, J., Bennett, G. N., San, K. Y. "Heterologous pyc expression under various promoters and through promoter engineering in a high succinate producing recombinant E. coli strain." Journal of BiotechnologyIn Press

     
     

    Wang, J., Zhu, J., Gennett, G. N., San K. Y. "Succinate production from different carbon sources under anaerobic conditions by metabolic engineered Escherichia coli strains." Metabolic Engineering, 13 (2011) : 328-335.

     
     

    Wang, J., Zhu, J., Gennett, G. N., San K. Y. "Succinate production from sucrose by metabolic engineered Escherichia coli strains under aerobic conditions." Biotechnology Progress, 27 (2011) : 1242-1247.

     
     

    Zhu, J. Sanchez, A., Bennett, G. N., & San K. Y. "Manipulating respiratory levels in Escherichia coli for aerobic formation of reduced chemical products." Metabolic Engineering, 13 (2011) : 704-712.

     
     

    Heath AP, Bennett GN, Kavraki LE "Finding metabolic pathways using atom tracking." Bioinformatics, 26: 1548-1555.

     
     

    Martinez, I., Lee, A., Bennett, G. N., San, K. Y "Effect of culture conditions on succinate pproduction by a high succinate producing Escherichia coli strain." Biotechnology & BioengineeringIn Press

     
     

    Martínez I, Bennett GN, San KY "Metabolic impact of the level of aeration during cell growth on anaerobic succinate production by an engineered Escherichia coli strain." Metab Eng, 12: 499-509.

     
     

    Zhu, J., Thakker, C., Bennett, G. N., San, K.Y. "Culture condition optimizations for the production of succinate in fed-batch cultures using a metabolic engineered Escherichia coli strain." Submitted

     
     

    Dittrich, C.R., Bennett, G.N., San, K.Y. "Metabolic engineering of the anaerobic central metabolic pathway in Escherichia coli for the simultaneous anaerobic production of isoamyl acetate and succinic acid." Biotechnology ProgressIn Press

     
     

    Park, Y.C., Shaffer, C.E.H., Bennett, G.N. "Microbial formation of esters." Applied Microbiology and BiotechnologyIn Press

     
     

    Portle, S., Adevaia, S., San, K.Y., Bennett, G. N., Mantzaris, N. "Environmentally modulated changes in fluorescence distribution in cells with oscillatory genetic network dynamics." J Biotechnol, 140 (2009) : 203-217.

     
     

    Sullivan, L., Bennett, G.N. "Local structural features of the Clostridium acetobutylicum ATCC825 butyrate kinase isozymes distinguish their enzymatic properties." Submitted

     
     

    Dittrich, C.R., Bennett, G.N., San, K.-Y. "Metabolic Engineering of the Anaerobic Central Metabolic Pathway in Escherichia coli for the Simultaneous Anaerobic Production of Isoamyl Acetate and Succinic Acid."  (2008) Submitted

     
     

    Iyer, A.V., Blinkova, A.L., Yang, S.-Y., Harrison, M., Tepp, W.T., Jacobson, M.J., Johnson, E.A., Bennett, G.N., and Walker, J.R. "Clostridium taeniosporum Is a Close Relative of the Clostridium botulinum Group II." Anaerobe (2008) In Press

     
     

    Martínez, I., Zhu, J., Lin, H., Bennett, G.N., San, K.-Y. "Replacing Escherichia coli NAD-dependent Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) with a NADP-dependent Enzyme from Clostridium acetobutylicum Facilitates NADPH Dependent Pathways." Metabolic Engineering, Epub Sept 23 (2008)

     
     

    Nikel, P.I., Zhu, J., San, K.-Y., Méndez, B.S., and Bennett, G.N. "Metabolic Flux Analysis of Carbon Catabolism in Escherichia coli creB and arcA Mutants Based on 13C-Labeling Experiments Under Microaerobic Conditions."  (2008) Submitted

     
     

    Portle, S., Iadevaia, S., San, K.-Y., Bennett, G.N., Mantzaris, N. "Environmentally-modulated Changes in Fluorescence Distribution in Cells with Oscillatory Network Dynamics." J. Biotechnology (2008) In Press

     
     

    Scotcher, M.C., and Bennett, G.N. "Activity of abrB310 Promoter in Wild Type and spo0A-deficient Strains of Clostridium acetobutylicum." J Ind Microbiol Biotechnol, 35 (2008) : 743-750.

     
     

    Singh, R., Vadlani, P.V., Harrison, M. L., Bennett, G.N., San, K.-Y "Aerobic Production of Isoamyl Acetate by Overexpression of the Yeast Alcohol Acetyl-transferase AFT1 and AFT2 in Escherichia coli and Using Low-cost Fermentation Ingredients." Bioprocess and Biosystems Engineering, 31 (2008) : 299-306.

     
     

    Wong, M.S., Causey, T.B., Bennett, G.N., and San, K.-Y "Engineering Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Copolymer Composition in E. coli." Biotechnology and Bioengineering, 99 (2008) : 919-928.

     
     

    Wong, M.S., Wu, S., Causey, T.B., Bennett, G.N., and San, K.-Y. "Reduction of Acetate Accumulation in Escherichia coli Cultures for Increased Recombinant Protein Production." Metabolic Engineering, 10 (2008) : 97-108.

     
     

    Kutty, R., and Bennett, G.N. "Characterization of a novel ferredoxin with N-terminal extension from Clostridium acetobutylicum ATCC 824." Arch. Microbiol., 187 (2007) : 161-169.

     
     

    Park, Y.C., San, K.-Y., and Bennett, G.N. "Characterization of alcohol dehydrogenase 1 and 3 from Neurospora crassa FGSC2489." Appl. Microbiology Biotechnology, 76 (2007) : 349-356.

     
     

    Portle, S., Causey, T.B., Wolf, K., Bennett, G.N., San, K.-Y., and Mantzaris, N. "Cell population heterogeneity in expression of a gene-switching network with fluorescent markers of different half-lives." J. Biotechnol., 128 (2007) : 362-375.

     
     

    Singh, R., Lemm, J., Bennett, G., and San, K.-Y. "Formate effect in metabolic flux redistribution in Escherichia coli under aerobic conditions."  (2007) In Press

     
     

    Singh, R., Vadlani, P.V., Harrison, M.L., Bennett, G.N., and San K.-Y. "Aerobic production of isoamyl acetate by overexpression of the yeast alcohol acetyl-transferase AFT1 and AFT2 in Escherichia coli and using low-cost fermentation ingredients." Bioprocess and Biosystems Engineering (2007) In Press

     
     

    Sullivan, L., Paredes, C.J., Papoutsakis, E.T., and Bennett, G.N. "Analysis of the clostridial hydrophobic with a conserved W family (ChW) of proteins in Clostridium acetobutylicum with emphasis on chw14 and chw16/17." Enzyme and Microbial Technology, 42 (2007) : 29-43.

     
     

    Wong, M.S., Causey, T.B., Mantzaris, N., Bennett, G.N., and San, K.-Y. "Engineering poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer composition in E. coli." Biotechnol. Bioeng (2007) In Press

     
     

    Wong, M.S., Wu, S., Causey, T.B., Bennett, G.N., and San, K.-Y. "Reduction of acetate production in Escherichia coli for increased recombinant protein production."  (2007) In Press

     
     

    Zhu, J., Levanon, S.S., Bennett, G., and San, K.-Y. "The YfiD protein contributions to the pyruvate formate-lyase flux in an E. coli arcA mutant strain." Biotechnol. Bioeng, 97 (2007) : 138-143.

     
     

    Cox, S.J., Shelel Levanon, S., Sanchez, A., Lin, H., Peercy, B., Bennett, G.N., and San, K.-Y. "Development of a metabolic network design and optimization framework incorporating implementation constraints: A succinate production case study." Metab. Eng., 8 (2006) : 46-57.

     
     

    Horton, C.E., and Bennett, G.N. "Ester production in E. coli and C. acetobutylicum." Enzyme & Microbial Technology, 38 (2006) : 368-376.

     
     

    Kutty, R., and Bennett, G.N. "Studies on inhibition of transformation of 2,4,6-trinitrotoluene by Fe-only hydrogenase from Clostridium acetobutylicum." J. Ind. Microbiol. Biotechnol., 33 (2006) : 368-276.

     
     

    Lin, H., Castro, N.M., Bennett, G.N., and San, K.-Y. "Acetyl-CoA synthetase overexpression in Escherichia coli demonstrates more efficient acetate assimilation and lower acetate accumulation: A potential tool in metabolic engineering." Applied Microbiology & Biotechnology, 71 (2006) : 870-874.

     
     

    Oh, Y., Varmanen, P., Han, X.-Y., Bennett, G., Xu, Z., Lu, T., and Palva, A. "Lactobacillus plantarum for oral peptide delivery." Oral Microbiology and Immunology, 21 (2006) : 1-5.

     
     

    Park, Y.C., Choi, J.H., Bennett, G.N., and Seo, J.H. "Characterization of d-ribose biosynthesis in Bacillus subtilis JY200 deficient in transketolase gene." J. Biotechnology, 121 (2006) : 508-516.

     
     

    Park, Y.C., Yun, N.R., San, K.-Y., and Bennett, G.N. "Molecular cloning and characterization of the alcohol dehydrogenase ADH1 gene of Candida utilis ATCC 9950." J. Ind. Microbiol. Biotechnol., 33 (2006) : 1032-1036.

     
     

    Peercy, B.E., Cox, S.J., Shalel-Levanon, S., San, K.-Y., and Bennett, G. "A kinetic model of oxygen regulation of cytochrome production in Escherichia coli." J. Theoretical Biol., 242 (2006) : 546-563.

     
     

    Sánchez, A.M., Andrews, J., Hussein, I., Bennett, G.N., and San, K.-Y. "Effect of overexpression of a soluble pyridine nucleotide transhydrogenase (UdhA) on the production of poly (3-hydroxybutyrate) in Escherichia coli." Biotechnol. Prog., 22 (2006) : 420-425.

     
     

    Sanchez, A.M., Bennett, G.N., and San, K.-Y. "Batch culture characterization and metabolic flux analysis of efficient succinate-producting Escherichia coli strains." Metab. Eng., 8 (2006) : 209-226.

     
     

    Shalel Levanon, S., San, K.-Y., and Bennett, G.N. "Effect of oxygen on the Escherichia coli ArcA and FNR regulation systems and metabolic responses." Biotechnol. Bioeng., 89 (2006) : 556-564.

     
     

    Singh, R., Yang, Y.-T., Lu, B., Bennett, G.N., and San, K.-Y. "Expression of the pfl gene and resulting metabolite flux distribution in nuo and ackA-pta E. coli mutant strains." Biotechnol. Prog., 22 (2006) : 898-29.

     
     

    Sullivan, L., and Bennett, G.N. "Proteome analysis and comparison of Clostridium acetobutylicum ATCC824 and Spo0A strain variants." J. Ind. Microbiol. Biotechnol., 33 (2006) : 298-308.

     
     

    Zhu, J., Levanon, S.S., Bennett, G., and San, K.-Y. "Effect of the global redox sensing/regulation networks on Escherichia coli and metabolic flux distribution based on C-13 labeling experiments." Metab. Eng., 8 (2006) : 619-627.

     
     

    Ali, M.K., Rudolph, F.B., and Bennett, G.N. "Characterization of thermostable Xyn10A enzyme from mesophilic Clostridium acetobutylicum strain ATCC 824." J. Ind. Microbiol. Biotechnol., 32 (2005) : 12-18.

     
     

    Cox, S.J., Shalel Levanon, S., Bennett, G.N., and San, K.-Y. "Genetically constrained metabolic flux analysis." Metabolic Engineering, 7 (2005) : 445-456.

     
     

    Dittrich, C.R., Bennett, G.N., and San, K.-Y. "Characterization of the acetate-producing pathways in Escherichia coli." Biotechnology Progress, 21 (2005) : 1062-1067.

     
     

    Dittrich, C.R., Vadali, R.V., Bennett, G.N., and San, K.-Y. "Redistribution of metabolic fluxes in the central aerobic metabolic pathway of E. coli mutant strains with deletion of the ackA-pta and poxB pathways for the synthesis of isoamyl acetate." Biotechnology Progress, 21 (2005) : 627-631.

     
     

    Kutty, R., and Bennett, G.N. "Biochemical characterization of trinitrotoluene transforming oxygen-insensitive nitroreductases from Clostridium acetobutylicum ATCC 824." Arch. Microbiol., 184 (2005) : 158-167.

     
     

    Kutty, R., and Bennett, G.N. "Studies on inhibition of transformation of 2,4,6-trinitrotoluene by Fe-hydrogenase from Clostridium acetobutylicum." J. Ind. Microbiol. Biotechnol. (2005) In Press

     
     

    Lin, H., Bennett, G.N., and San, K.-Y. "Chemostat culture characterization of Escherichia coli mutant strains metabolically engineered for aerobic succinate production: A study of the modified metabolic network based on metabolite profile, enzyme activity, and gene expression profile." Metabolic Engineering, 7 (2005) : 337-352.

     
     

    Lin, H., Bennett, G.N., and San, K.-Y. "Genetic reconstruction of the aerobic central metabolism in Escherichia coli for the absolute aerobic production of succinate." Biotechnology & Bioengineering, 89 (2005) : 148-156.

     
     

    Lin, H., Bennett, G.N., and San, K.-Y. "Metabolic engineering of aerobic succinate production systems in Escherichia coli to improve process productivity and achieve the maximum theoretical succinate yield." Metabolic Engineering, 7 (2005) : 116-127.

     
     

    Lin, H., Bennett, GN., and San, K.-Y. "Effect of Sorghum vulgare phosphoenolpyruvate carboxylase and Lactococcus lactis pyruvate carboxylase on metabolite production in Escherichia coli." Applied Microbiology & Biotechnology, 67 (2005) : 515-523.

     
     

    Lin, H., Bennett, GN., and San, K.-Y. "Effect of carbon sources differing in oxidation state and transport route on succinate production in metabolically engineered Escherichia coli." J. Ind. Microbiol. Biotechnol., 32 (2005) : 87-93.

     
     

    Lin, H., Bennett, GN., and San, K.-Y. "Fed batch culture of a metabolically engineered Escherichia coli strain designed for high level succinate production and yield under aerobic conditions." Biotechnology Bioengineering, 90 (2005) : 775-779.

     
     

    Sanchez, A.M., Bennett, G.N., and San, K.-Y. "Batch culture characterization and metabolic flux analysis of efficient succinate production Escherichia coli strains."  (2005) Submitted

     
     

    Sanchez, A.M., Bennett, G.N., and San, K.-Y. "Effect of different levels of NADH availability on metabolic fluxes of Escherichia coli chemostat cultures in defined medium." J. Biotechnol., 117 (2005) : 395-405.

     
     

    Sanchez, A.M., Bennett, G.N., and San, K.-Y. "Efficient succinate acid production from glucose through overexpression of pyruvate carboxylase in an Escherichia coli alcohol dehydrogenase and lactate dehydrogenase mutant." Biotechnology Progress, 21 (2005) : 358-365.

     
     

    Sanchez, A.M., Bennett, G.N., and San, K.-Y. "Novel pathway engineering design of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity." Metabolic Engineering, 7 (2005) : 229-239.

     
     

    Scotcher, M.C., Rudolph, F.B., and Bennett, G.N. "Expression of abrB310 and sinR, and effects of decreased abrB310 expression on the transition from acidogenesis to solventogenesis, in Clostridium acetobutylicum ATCC824." Appl. Environ. Microbiol., 71 (2005) : 1987-1995.

     
     

    Scotcher, M.C., and Bennett, G.N. "SpoIIE regulates sporulation but does not directly affect solventogenesis in Clostridium acetobutylicum ATCC824." J. Bacteriol., 187 (2005) : 1930-1936.

     
     

    Shalal Levanon, S., San, K.-Y., and Bennett, G.N. "Effect of oxygen on the E. coli ArcA and FNR regulation systems and metabolic responses." Biotechnology and Bioengineering, 89 (2005) : 556-564.

     
     

    Shalel-Levanon, S., San, K.-Y., and Bennett, G.N. "Effect of ArcA and FNR on the expression of genes related to the oxygen regulation and the glycolysis pathway in Escherichia coli under microaerobic growth conditions." Biotechnology Bioengineering, 92 (2005) : 147-159.

     
     

    Shalel-Levanon, S., San, K.-Y., and Bennett, G.N. "Effect of oxygen, and ArcA and FNR regulators on the expression of genes related to the electron transfer chain and the TCA cycle in Escherichia coli." Metabolic Engineering, 7 (2005) : 364-374.

     
     

    Singh, R., Lemm, J., Bennett, G., and San, K.-Y. "Formate Effect in Metabolic Flux Redistribution in Escherichia coli Under Aerobic Conditions."  (2005) Submitted

     
     

    Sullivan, L., and Bennett, G.N. "Proteome analysis and comparison of Clostridium acetobutylicum ATCC 824 and Spo0A strain variants." J. Ind. Microbiol. Biotechnol. (2005) In Press

     
     

    Vadali, R.V., Fu. Y., Bennett, G.N., and San, K.Y. "Enhanced lycopene productivity by manipulation of carbon flow to isopentenyl diphosphate in Escherichia coli." Biotechnology Progress, 21 (2005) : 1558-1561.

     
     

    Yun, N.-R., San, K.-Y., and Bennett, G.N. "Enhancement of lactate and succinate formation in adhE or pta-ackA mutants of NADH dehydrogenase-deficient Escherichia coli." Journal of Applied Microbiology, 99 (2005) : 1404-1412.

     
     

    Zhang, C., and Bennett, G.N. "Biodegradation of xenobiotics by anaerobic bacteria." Applied Microbiology & Biotechnology, 67 (2005) : 600-618.

     
     

    Zhao, Y., Tomas, C.A., Rudolph, F.B., Papoutsakis, E.T., and Bennett, G.N. "Intracellular butyryl phosphate and acetyl phosphate concentrations in Clostridium acetobutylicum and implications for solvent formation." Appl. Environ. Micro., 71 (2005) : 530-537.

     
     

    Ali, M.K., Rudolph, F.B., and Bennett, G.N. "Thermostable xylanase10B from Clostridium acetobutylicum ATCC824." J. Ind. Microbiol. Biotechnol., 31 (2004) : 229-234.

     
     

    Berrios-Rivera, S.J., Sanchez, A.M., Bennett, G.N., and San, K.-Y. "Effect of different levels of NADH availability on metabolite distribution in Escherichia coli on minimal and complex media." Appl. Microbiol. Biotechnol., 65 (2004) : 426-432.

     
     

    Cox, S.J., Shalel Levanon, S.S., Bennett, G.N., and San, K.-Y. "Genetic network assisted metabolic flux analysis." Metabolic Engineering (2004) In Press

     
     

    Dittrich, C.R., San, K.-Y., and Bennett, G.N. "Cofactors in metabolic engineering of ester production." Proceedings of the American Chemical Society Symposium on Genetic Engineering in Flavor Chemistry: Applications and Potentials, ACS Press (2004) In Press

     
     

    Dittrich, C.R., Vadali, R.V., Bennett, G.N., and San, K.-Y. "Redistribution of metabolic fluxes in the central aerobic metabolic pathway of E. coli mutant strains with deletion of the ackA-pta and poxB pathways for the synthesis of isoamyl acetate." Biotechnology Progress (2004) In Press

     
     

    Horton, C.H., and Bennett, G.N. "Ester production in E. coli and C. acetobutylicum."  (2004) Submitted

     
     

    Kutty, R., and Bennett, G.N. "Studies on inhbition of transformation of 2,4,6-trinitrotoluene by Fe-hydrogenase from Clostridium acetobutylicum."  (2004) Submitted

     
     

    Lin, H., Bennett, GN., and San, K.-Y. "Effect of Sorghum phosphoenolpyruvate carboxylase and Lactococcus lactis pyruvate carboxylase on metabolite production in Escherichia coli." Applied Microbiology & Biotechnology (2004) In Press

     
     

    Lin, H., Vadali, R.V., Bennett, G.N., and San, K.-Y. "Increasing the acetyl-CoA pool in the presence of overexpressed phosphoenolpyruvate carboxylase or pyruvate carboxylase enhances succinate production in Escherichia coli." Biotechnol. Prog., 20 (2004) : 1599-1604.

     
     

    Sanchez, A.M., Bennett, G.N., and San, K.-Y. "Efficient succinate acid production from glucose through overexpression of pyruvate carboxylase in an Escherichia coli alcohol dehydrogenase and lactate dehydrogenase mutant." Biotechnology & Bioengineering (2004) In Press

     
     

    Sanchez, A.M., Bennett, G.N., and San, K.-Y. "Novel pathway engineering design of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity,."  (2004) Submitted

     
     

    Scotcher, M.C., Rudolph, F.B., and Bennett, G.N. "The expression of abrB310 and sinR, and the effects of AbrB310 on the transition from acidogenesis to solventogenesis in Clostridium acetobutylicum ATCC824." Applied & Environ. Microbiol. (2004) In Press

     
     

    Vadali, R.V., Bennett, G.N., and San, K.-Y. "Applicability of CoA/acetyl-CoA manipulation system to enhance isoamyl acetate production in Escherichia coli." Metabolic Engineering, 6 (2004) : 294-299.

     
     

    Vadali, R.V., Bennett, G.N., and San, K.-Y. "Cofactor engineering of CoA/Acetyl-CoA and its effect on metabolic flux redistribution in Escherichia coli." Metabolic Eng., 6 (2004) : 133-139.

     
     

    Vadali, R.V., Bennett, G.N., and San, K.-Y. "Enhanced isoamyl acetate production upon manipulation of the acetyl-CoA node in Escherichia coli." Biotechnol. Prog., 20 (2004) : 692-697.

     
     

    Vadali, R.V., Horton, C.E., Rudolph, F.B., Bennett, G.N., and San, K.Y. "Production of isoamyl acetate in ackA-pta and/or ldh mutants of Escherichia coli with overexpression of yeast ATF2." Appl. Microbiol. Biotechnol., 63 (2004) : 698-704.

     
     

    Yun, N.-R., San, K.-Y., and Bennett, G.N. "Effect of inactivation of adhE or pta-ackA in NADH dehydrogenase-deficient Escherichia coli on the pattern of anaerobic metabolic products."  (2004) Submitted

     
     

    Zhao, Y., Tomas, C.A., Rudolph, F.B., Papoutsakis, E.T., and Bennett, G.N. "Intracellular butyryl- phosphate and acetyl-phosphate concentrations in Clostridium acetobutylicum and implications for solvent formation." Applied and Environ. Micro. (2004) In Press

     
     

    Ali, M.K., Rudolph, F.B., and Bennett, G.N. "Cloning and expression of the gene encoding the Clostridium acetobutylicum thermostable xylanase 10B, a broad substrate specificity family 10 glycosyl hydrolase."  (2003) Submitted

     
     

    Berrios-Rivera, S.J., San, K.-Y., and Bennett, G.N. "The effect of carbon sources and lactate dehydrogenase deletion on 1,2-propanediol production in Escherichia coli." Journal of Industrial Microbiology and Biotechnology, 30 (2003) : 34-40.

     
     

    Horton, C.E., Huang, K.-X., Bennett, G.N., and Rudolph, F.B., "Heterologous expression of the Saccharomyces cerevisiae alcohol acetyltransferase genes in Clostridium acetobutylicum and Escherichia coli for the production of isoamyl acetate." Journal of Industrial Microbiology and Biotechnology, 30 (2003) : 427-432.

     
     

    Kim, H.-Y., Bennett, G.N., and Song, H.-G. "Degradation of 2,4,6-trinitrotoluene by Enterobacter sp. strain C1 isolated from activated sludge." Biotechnology Letters, 24 (2003) : 2023-2028.

     
     

    Padda, R.S., Wang, C., Hughes, J.B., Kutty, R., and Bennett, G.N. "Mutagenicity of nitroaromatic degradation compounds." Environmental Toxicology and Chemistry, 22 (2003) : 2293-2297.

     
     

    Scotcher, M., Huang, K.-X., Harrison, M.L., Rudolph, F.B., and Bennett, G.N. "Sequences affecting the regulation of solvent formation in Clostridium acetobutylicum." Journal of Industrial Microbiology and Biotechnology, 30 (2003) : 414-420.

     
     

    Vadali, R.V., Bennett, G.N., and San, K.-Y. "Applicability of CoA/Acetyl-CoA Manipulation System to Enhance Isoamyl Acetate Production in Escherichia coli."  (2003) Submitted

     
     

    Vadali, R.V., Bennett, G.N., and San, K.-Y. "Cofactor Engineering of CoA/Acetyl-CoA and its Effect on Metabolic Flux Redistribution in Escherichia coli."  (2003) Submitted

     
     

    Vadali, R.V., Bennett, G.N., and San, K.-Y. "Enhanced Isoamyl Acetate Production upon Manipulation of the Acetyl-CoA Node in Escherichia coli."  (2003) Submitted

     
     

    Vadali, R.V., Horton, C.E., Rudolph, F.B., Bennett, G.N., and San, K.Y. "Production of isoamyl acetate in ackA-pta and/or ldh mutants of Escherichia coli with overexpression of yeast ATF2." Appl. Microbiol. Biotechnol. (2003) : Epub ahead of print.

     
     

    Wang, C., Lyon, D.Y., Hughes, J.B., and Bennett, G.N. "Role of hydroxylamino-intermediates in the phytotransformation of 2,4,6-trinitrotoluene in Myriophyllum aquaticum." Environmental Toxicology and Chemistry, 37 (2003) : 3595-5600.

     
     

    Watrous, M.M., Clark, S., Kutty, R., Huang, S., Rudolph, F.B., Hughes, J.B., and Bennett, G.N., "2,4,6-Trinitrotoluene reduction by an Fe-only hydrogenase in Clostridium acetobutylicum." Applied and Environmental Microbiology, 69 (2003) : 1542-1547.

     
     

    Zhao, Y., Hindorff, L.A., Chuang, A., Monroe-Augustus, M., Lyristis, M., Harrison, M., Rudolph, F.B., and Bennett, G.N. "Expression of a cloned cyclopropane fatty acid synthase gene reduces solvent formation in Clostridium acetobutylicum ATCC 824." Applied and Environmental Microbiology, 69 (2003) : 2831-2841.

     
     

    Berrios-Rivera, S.J., Bennett, G.N., and San, K.-Y. "Metabolic engineering of Escherichia coli through genetic manipulation of NADH availability." Metabolic Engineering, 4 (2002) : 217-229.

     
     

    Berrios-Rivera, S.J., Bennett, G.N., and San, K.-Y. "The effect of manipulating NADH availability on the redistribution of metabolic fluxes in Escherchia coli chemostat cultures." Metabolic Engineering, 4 (2002) : 230-237.

     
     

    Berrios-Rivera, S.J., San, K.-Y., and Bennett, G.N. "The effect of NAPRTase overexpression on the total levels of NAD, NADH/NAD+ ratio, and the distribution of metabolites in Escherichia coli." Metabolic Engineering, 4 (2002) : 238-247.

     
     

    Horton, C.E., Rudolph, F., and Bennett, G. "Cloning and expression of genes encoding yeast alcohol acetyltransferases in E. coli and Clostridium acetobutylicum." Journal of Industrial Microbiology and Biotechnology (2002) Submitted

     
     

    Oh, Y., Osato, M.S., Han, X., Bennett, G., and Hong, W.K. "Folk yoghurt kills Helicobacter pylori." J. Appl. Microbiol., 93 (2002) : 1083-1088.

     
     

    Padda, R.S., Wang, C., Hughes, J.B., and Bennett, G.N. "Mutagenicity of nitroaromatic explosives during anaerobic transformation by Clostridium acetobutylicum." Environmental Toxicology and Chemistry (2002) Submitted

     
     

    San, K.-Y., Bennett, G.N., Berrios-Rivera, S.J., Vadali, R.V., Yang, Y.-T., Horton, E., Rudolph, F.B., Sariyar, B., and Blackwood, K. "Metabolic engineering through cofactor manipulation and its effects on metabolic flux redistribution in Escherichia coli." Metabolic Eng., 4 (2002) : 182-192.

     
     

    Wang, C., Bennett, G.N., and Hughes, J.B. "The role of hydroxylamino-intermediates in the phytotransformation of 2,4,6-trinitrotoluene in Myriophyllum aquaticum." Environmental Toxicology and Chemistry (2002) Submitted

     
     

    Yang, Y.-T., Bennett, G.N., and San, K.-Y. "Expressional analysis of Escherichia coli genome during low and high cell density growth."  (2002) Submitted

     
     Books
     

    Papoutsakis, E.T., and Bennett, G.N. "Metabolic Engineering of Clostridium acetobutylicum."  (1999) : 253-279.

     
     Book chapters
     

    San, K. Y. and Bennett, G. N. "Metabolic engineering of biochemical pathways." Encyclopedia of Science and Technology (2014 )

     
     

    Thakker, C., San, K. Y., Bennett, G. N. "Soybean carbohydrates as a renewable feedstock for the fermentative production of succinic acid and ethanol." Chapter 4 in American Chemical Society Symposium Series 1178 (2014) : 81-107 .


    doi: 10.1021/bk-2014-1178.ch004
     
     

    George N. Bennett and Ka -Yiu San "Enzyme and Co-factor Engineering: Current trends and Future Prospects in the Pharmaceutical and Fermentation Industries." Fermentation Microbiology and Biotechnology (third edition), Ch. 8In Press

     
     

    Bennett, G.N. and San, K.-Y., "Engineering E. coli central metabolism for enhanced primary metabolite production," Chapter 17 in Systems Biology and Biotechnology of Escherichia coli, Lee, S. Y. (ed.), Springer Publishing (2009), 351-376.

     
     

    Nikel, P.I., Zhu, J., San, K.Y. Mendez, B.S., Bennett, G.N. "Metabolic flux analysis of Escherichia coli creB and arcA mutants reveals shared control of carbon catabolism under microaerobic growth conditions." In Press

     
     

    Bennett G. N., & San, K.-Y. "Engineering E. coli Central Metabolism for Enhanced Primary Metabolite Production." Chaper 17 in Systems Biology and Biotechnology of E. coli (2008) : 351-375.

     
     

    Sullivan, L., Scotcher, M.C., and Bennett, G.N, "Increased biofuel production by metabolic engineering of Clostridium acetobutylicum." Microbial Biofuel Conversion: Microbial Contributions to Alternative Fuel (2008) : 361-376.

     
     

    Bennett, G.N., and San, K.-Y. "Enzyme and co-factor engineering and their applications in the pharmaceutical and fermentation industries." Chapter 8, Fermentation Microbiology and Biotechnology 2nd edition, (E.M.T. El-Mansi, ed.), Taylor & Francis Pub., Oxford UK (2006) : 217-248.

     
     

    Ahmad, F., Hughes, J.B., and Bennett, G.N. "Biodegradation of hazardous materials by Clostridia." Handbook on Clostridia, Chap. 38 (P. Durre, ed.), CRC Press, Boca Raton, Florida (2005) : 831-854.

     
     

    San, K.-Y., and Bennett, G.N. "Expression systems for DNA processes." Encyclopedia of Molecular Cell Biology and Molecular Medicine: Vol. 4 (R.A. Meyers, ed), Wiley-VCH, Wieuheim, Germany (2004) : 355-377.

     
     Conference papers
     

    Dittrich, C.R., San, K.-Y., and Bennett, G.N. "Cofactors in metabolic engineering of ester production." Proceedings of the American Chemical Society Symposium on Genetic Engineering in Flavor Chemistry: Applications and Potentials, ACS Press (2006) In Press

     
     

    Heath, A.P., Bennett, G.N., and Kavraki, L.E. "Visualizing the Results of Metabolic Pathway Queries."  (2008) In Press

     
     

    Kutty, R., Rudolph, F.B., and Bennett, G.N. "Transformation of 2,4,6-trinitrotoluene by Fe-hydrogenase from Clostridium acetobutylicum ATCC824: Relevance to mechanism of catalysis." Proceedings Remediation of Chlorinated and Recalcitrant Compounds: The Fourth International Conference, May 24-27, 2004, Monterey, California, Battelle Press, Columbus, OH (2005)

     
     Other
     

    Bennett, G.N., and San, K-Y. "Microbial Formation, Biotechnological Production and Applications of 1,2-Propanediol." Appl. Microbiol. Biotechnol., 55 (2001) : 1-9.

     
     

    Berrios-Rivera, S.J., Bennett, G.N., and San, K.-Y. "Metabolic Engineering of Escherichia coli Through Genetic Manipulation of NADH Availability."  (submitted)

     
     

    Berrios-Rivera, S.J., Bennett, G.N., and San, K.-Y. "The Effect of Manipulating NADH Availability on the Redistribution of Metabolic Fluxes in Escherchia coli Chemostat Cultures."  (submitted)

     
     

    Berrios-Rivera, S.J., San, K.-Y., and Bennett, G.N. "The Effect of Carbon Sources and Lactate Dehydrogenase Deletion on 1,2-Propanediol Production in Escherichia coli."  (submitted)

     
     

    Berrios-Rivera, S.J., San, K.-Y., and Bennett, G.N. "The Effect of NAPRTase Overexpression on the Total Levels of NAD, NADH/NAD+ Ratio, and the Distribution of Metabolites in Escherichia coli."  (submitted)

     
     

    Loke, H.-K., Bennett, G.N., and Lindahl, P.A. "Active Acetyl-CoA Synthase from Clostridium thermoaceticum Obtained by Cloning and Heterologous Expression of acsAB in Escherichia coli." Proc. National Acad. Sci. USA, 97 (2000) : 12530-12535.

     
     

    Nolling, J., Breton, G., Omelchenko, M.V., Makarova, K.S., Zeng, Q., Gibson, R., Lee, H.M., Dubois, J., Qiu, D., Hitti, J., Wolf, Y.I., Tatusov, R.L., Sabathe, F., Doucette-Stamm, L., Soucaille, P., Daly, M.J., Bennett, G.N., Koonin, E.V., Smith, D.R. "Genome Sequence and Comparative Analysis of the Solvent-producing Bacterium Clostridium acetobutylicum." J. Bacteriol., 183 (2001) : 4823-1438.

     
     

    San, K.-Y., Bennett, G.N., Berrios-Rivera, S.J., Vadali, R.V., Yang, Y.-T., Horton, E., Rudolph, F.B., Sariyar, B., and Blackwood, K. "Metabolic Engineering Through Cofactor Manipulation and its Effects on Metabolic Flux Redistribution in Escherichia coli."  (in press)

     
     

    Tummala, S.B., Tomas, C., Harris, L.M., Welker, N.E., Rudolph, F.B., Bennett, G.N., and Papoutsakis, E.T. "Genetic Tools for Solventogenic Clostridia." In Clostridia - Biotechnology and Medical Applications (H. Bahl, P. Durre, eds.), Wiley-VCH, Weinheim, Germany (2001) : 105-123.

     
     

    Wardwell, S.A., Yang, Y.T., Chang, H.Y., San, K.Y., Rudolph, F.B., and Bennett, G.N. "Expression of the Klebsiella pneumoniae CG21 Acetoin Reductase Gene in Clostridium acetobutylicum ATCC 824." J. Ind. Microbiol. Biotechnol., 27 (2001) : 220-227.

     
     

    Yang, Y.-T., Bennett, G.N., and San, K.-Y. "Expressional Analysis of the Escherichia coli Genome During Low and High Cell Density Growth."  (in press)

     
     

    Yang, Y.-T., Bennett, G.N., and San, K.-Y. "The Effects of Feed and Intracellular Pyruvate Levels on the Redistribution of Metabolic Fluxes in Escherichia coli." Metabolic Engineering, 3 (2001) : 115-123.

     
     

    Berrios-Rivera, S.J., Yang, Y.-T., Bennett, G.N., and San, K.-Y. "Effect of Glucose Analog Supplementation on Metabolic Flux Distribution in Anaerobic Chemostat Cultures of Escherichia coli." J. Metabolic Engineering, 2 (2000) : 149-154.

     
     

    Huang, K.-x, Huang, S., Rudolph, F.B., and Bennett, G.N. "Identification and Characterization of a Second Butyrate kinase from Clostridium acetobutylicum ATCC 824." J. Molecular Microbiology and Biotechnology, 2 (2000) : 33-38.

     
     

    Huang, S., Lindahl, P.A., Wang, C., Bennett, G.N., Rudolph, F.B., and Hughes, J.B. "2,4,6-Trinitrotoluene Reduction by Carbon Monoxide Dehydrogenase from Clostridium thermoaceticum." Appl. Environ. Microbiol., 66 (2000) : 1474-1478.

     
     

    Lyristis, M., Boynton, Z.L., Petersen, D., Kan, Z., Bennett, G.N., and Rudolph, F.B. "Cloning, Sequencing and Characterization of the Gene Encoding Flagellin, flaC, and the Post-translational Modification of the Flagellin from Clostridium acetobutylicum ATCC 824." Anaerobe, 6 (2000) : 69-79.

     
     

    Padda, R.S., Wang, C.Y., Hughes, J.B., and Bennett, G.N. "Mutagenicity of Trinitotoluene and its Metabolites Formed During Anaerobic Degradation by Clostridium acetobutylicum ATCC 824." Environmental Toxicology and Chemistry, 19 (2000) : 2871-2875.

     
     

    Tyurin, M., Padda, R., Huang, K., Wardwell, S., Caprette, D., and Bennett, G.N. "Electrotransformation of Clostridium acetobutylicum ATCC 824 Using High-voltage Radio Frequency Modulated Square Pulses." J. Applied Bacteriol., 88 (2000) : 220-227.

     
     

    Yang, Y.-T., Peredelchuk, M., Bennett, G.N., and San, K.-Y. "Effect of Variation of Klebsiella pneumoniae Acetolactate Synthase Expression on Metabolic Flux Redistribution in Escherichia coli." Biotechnology & Bioengineering, 20 (2000) : 150-159.

     
     

    Aristidou, A.A., Bennett, G.N., and San, K.-Y. "Improvement of Biomass Yield and Recombinant Gene Expression in Escherichia coli by Using Fructose as the Primary Carbon Source." Biotech. Prog., 15 (1999) : 140-145.

     
     

    Aristidou, A.A., Bennett, G.N., and San, K.-Y. "Metabolic Flux Analysis of E. coli Expressing the B. subtilis ALS in Batch and Continuous Cultures." Biotech. Bioeng., 63 (1999) : 737-749.

     
     

    Huang, K-x., Scott, A.I., and Bennett, G.N. "Overexpression, Purification and Characterization of the Thermostable Mevalonate Kinase from Methanococcus jannaschii." Protein Expression and Purification, 17 (1999) : 33-40.

     
     

    Huang, K.-x., Rudolph, F.B., and Bennett, G.N. "Characterization of Methylglyoxal Synthase from Clostridium acetobutylicum ATCC 824 and Its Use in the Formation of 1,2-Propanediol." Appl. Environ. Microbiol., 65 (1999) : 3244-3247.

     
     

    Nair, R.V., Green, E.M., Watson, D.E., Bennett, G.N., and Papoutsakis, E.T. "Regulation of the Sol Locus Genes for Butanol and Acetone Formation in Clostridium acetobutylicum ATCC 824 by a Putative Transcriptional Repressor." J. Bacteriol., 181 (1999) : 319-330.

     
     

    Yang, Y.-T., Bennett, G.N., and San, K.-Y. "Effect of Inactivation of nuo and ackA-pta on Redistribution of Metabolic Fluxes in Escherichia coli." Biotech. Bioengineering, 65 (1999) : 291-297.

     
     

    Yang, Y.-T., San, K.-Y., and Bennett, G.N. "Metabolic Flux Analysis of E. coli Deficient in the Acetate Production Pathway and Expressing the B. subtilis Acetolactate Synthase." Metabolic Engineering, 1 (1999) : 26-34.

     
     

    Yang, Y.-T., San, K.-Y., and Bennett, G.N. "Redistribution of Metabolic Fluxes in Escherichia coli with LDHA Overexpression and Deletion." Metabolic Eng., 1 (1999) : 141-152.

     
     

    Belouski, E., Gui, L., Rudolph, F.B., and Bennett, G.N. "Complementation of an Escherichia coli Polypeptide Deformylase Mutant with a Gene from Clostridium acetobutylicum ATCC 824." Current Microbiol., 36 (1998) : 248-249.

     
     

    Belouski, E., Watson, D.E., and Bennett, G.N. "Cloning, Sequence and Expression of the Phosphofructokinase Gene of Clostridium acetobutylicum ATCC 824 in Escherichia coli." Current Microbiol., 37 (1998) : 17-22.

     
     

    Green, E.M., and Bennett, G.N. "Genetic Manipulation of Acid and Solvent Formation in Clostridium acetobutylicum ATCC 824." Biotechnology & Bioengineering, 58 (1998) : 215-221.

     
     

    Hughes, J.B., Wang, C., Bhadra, R., Richardson, A., Bennett, G., and Rudolph, F. "Reduction of 2,4,6-Trinitrotoluene by Clostridium acetobutylicum Through Hydroxylamino-nitrotoluene Intermediates." Environ. Tech. & Chem., 17 (1998) : 343-348.

     
     

    Hughes, J.G., Wang, C., Yesland, K., Richardson, A., Bhadra, R., Bennett, G., and Rudolph, F. "Bamberger Rearrangement During TNT-metabolism by Clostridium acetobutylicum." Environ. Sci. & Tech., 32 (1998) : 494-500.

     
     

    Yang, Y.-T., Bennett, G.N., and San, K.-Y. "Genetic and Metabolic Engineering." Electronic Journal of Biotechnology, 3 (1998)

     
    Presentations
     Invited Talks
     

    "Hosts and Expression Systems for Metabolic Engineering ." IIT Kharagpur, Kharagpur, India. (December 10, 2014)

     
     

    "Manipulation of redox cofactors in Clostridium acetobutylicum ATCC824 ." Clostridium XIII Conference, Shanghai, China. (September 19, 2014)

     
     

    "Manipulation of redox in microbial cells." Bacteria Interest Group (BIG Meeting), UTHSC, Houston TX. (March 5, 2014)

     
     

    "Manipulation of redox in microbial cells." Shanghai Jiao Tong University, Shanghai, China. (September 22, 2014)

     
     

    "Production of chemicals by controlling redox in microbial conversions.." East China College of Science & Technology, Shanghai, China. (September 18, 2014)

     
     

    "Rice succinate soy project." South Dakota Innovation Partners- Prairie Aquatech, Brookings SD. (November 13, 2014)

     
     

    "Successful Examples of Metabolic Engineering ." IIT Kharagpur, Kharagpur, India. (December 12, 2014)

     
     

    "Systems Metabolic Engineering ." IIT Kharagpur, Kharagpur, India. (December 17, 2014)

     
     

    "The Basics of Metabolic Engineering I." IIT Kharagpur, Kharagpur, India. (December 8, 2014)

     
     

    "The Basics of Metabolic Engineering II." IIT Kharagpur, Kharagpur, India. (December 9, 2014)

     
     

    "The Multi-scale Biomolecular Networks Research Experience at Rice University." San Jacinto College, Houston, TX. (November 6, 2014)

     
     

    Awardee, ACS-GHS Section Award.  "Microbial Production of Chemicals." Greater Houston Section, American Chemical Society , Houston, TX. (November 21, 2013)

     
     

    "The Multi-scale Biomolecular Networks Research Experience at Rice University." San Jacinto College, Houston, TX. (January 30, 2013)

     
     

    "Analysis of Redox Responses During TNT Transformation by Clostridium acetobutylicum ATCC824." Clostridium XII International Conference on the Genetics, Physiology and Biotechnology of Solvent- and Acid-forming Clostridia, Nottingham, UK. (September, 2012)

     
     

    "Biofuels: perspectives and opportunities." Focus the Nation Clean Energy Forum, University of St. Thomas, Houston, TX. (March 7, 2012)

     
     

    "Microbes can make many specialty chemicals." University Rotary International Group, Houston, TX. (October 17, 2012)

     
     

    "Microbial technologies for production of specialty chemicals." Celanese Corp., Pasadena, TX. (May 16, 2012)

     
     

    "Redox Responses and TNT Transformation by Clostridium acetobutylicum." Bacterial Interest Group, Houston, TX. (September 5, 2012)

     
     

    "Strategies for succinate production in E. coli." Opening Lecture, Symposium on Bio-based Production of Organic Acids, DECHEMA-House, Theodor-Heuss-Allee 25, Frankfurt, Germany. (May 10, 2012)

     
     

    "Modulating the clostridial butanol (ABE) fermentation for higher product value." 60th Meeting of the Society of Industrial Microbiology, San Francisco, CA. (August)

     
     

    "Clostridium acetobutylicum and biotechnology." First International Conference of Biological Sciences, ICBS-1, Assiut University, Egypt. (March 4-5, 2009)

     
     

    "Clostridium acetobutylicum and the ABE fermentation: approaches and trends." Cathay Industrial Biotech, Ltd., Shanghai, China. (October 17, 2008)

     
     

    "Clostridium acetobutylicum: biotechnology and ecological aspects." Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, China. (October 16, 2008)

     
     

    "Clostridium and butanol." Cobalt Biofuels, Palo Alto, California. (June 2, 2008)

     
     

    "Co-Production of riboflavin and butanol in Clostridium acetobutylicum 824." Clostricium 10, Wageningen, The Netherlands. (September 30, 2008)

     
     

    "Contribution of Clostridium to the degradation of hazardous compounds." Marie Curie Symposium: Non-Pathogenic Clostridia Congress, Toulouse, France. (February 24-28, 2008)

     
     

    "Genetic strategies related to succinate production in Escherichia coli." Roquette Freres-DSM meeting, Lestrum, France. (January 20, 2008)

     
     

    "Clostridium acetobutylicum and Butanol." Corporate and VC meeting, California. (September 23, 2007)

     
     

    "Clostridium acetobutylicum and the Production of Butanol." INRA, Rennes, France. (June 13, 2007)

     
     

    "Bioenergy Research at Rice University." Southeastern Universities Research Association: Southern Bioenergy Summit, Washington, D.C.. (February 27-28, 2007)

     
     

    "Biological Aspects of Deciphering and Engineering Regulatory Networks." Joint Meeting of DOE Genomics to Life: Systems Biology for Energy and Environment; USDA-DOE Plant Feedstock Genomics for Bioenergy Awardee Workshop 2007; and Metabolic Engineering Working Group Interagency Conference on Metabolic Engineering 2007, Bethesda, Maryland. (February 11-14, 2007)

     
     

    "Genetics and Biotechnology of Clostridium acetobutylicum." 15th Annual International Conference on Microbial Genomics, University of Maryland. (September 16-20, 2007)

     
     

    "Recent Genetic Work Related to Succinate Production in Escherichia coli." Business consortium, Europe. (June 7, 2007)

     
     

    "Analysis of Oxidative and Fermentative Pathways in Mutant E. coli and Application to Succinate Production." 3rd International E. coli Alliance Conference on Systems Biology (IECA2006), Juju, Korea. (October 31-November 4, 2006)

     
     

    "Biofuels and Chemicals from Sugars." 2nd Annual Rice Institute of Biosciences and Bioengineering Symposia, Rice University, Houston, Texas. (July 13, 2006)

     
     

    "Biomass to Chemical Production Systems." Rice Energy Symposium, EESI, Rice University, Houston, Texas. (January 10, 2006)

     
     

    "Butanol Production by Microbial Fermentation." 62nd Southwest Regional ACS Meeting, Houston, Texas. (October 20, 2006)

     
     

    "Butanol Production by Microbial Fermentation." Biomass to Chemicals and Fuels: Science, Technology and Public Policy Symposium, Baker Institute, Rice University. (Sepember 25-26, 2006)

     
     

    "Manipulation of Microbial Biochemistry for Chemical Production and Biodegradation." University of Missouri, Rolla, Missouri. (April 11, 2006)

     
     

    "Metabolic Engineering of Succinate Production in Escherichia coli." Archer-Daniels-Midland, Decatur, Illinois. (September 22, 2006)

     
     

    "Production of Chemicals by Microorganisms." Trinity University, San Antonio, Texas. (March 27, 2006)

     
     

    "Recent Genetic Work Related to Succinate Production and Other Chemicals in Escherichia coli." Roquette-Freres, Lestrum, France. (September 8, 2006)

     
     

    "Rice University and Biotechnology." BASF, Ludwigshafen, Germany. (February 16, 2006)

     
     

    "Engineering of Escherichia coli and Clostridium acetobutylicum to Produce Useful Chemicals." Dow AgroSciences, Indianapolis, Indiana. (August 8, 2005)

     
     

    "Metabolic Engineering of Succinate Production." DuPont, Wilmington, Delaware. (December 6, 2005)

     
     

    "Anaerobic biodegradation of hazardous compounds." Texas Envirochem, Houston, Texas. (October 13, 2004)

     
     

    "Assessment of clostridial action on hazardous substances." Army Research Office Life Sciences Division "Sensing and Processing by Neuronal and Microbial Communities", Cashiers, North Carolina. (April 25-28, 2004)

     
     

    "Cofactors in metabolic engineering of ester production." Symposium on Genetic Engineering in Flavor Chemistry: Applications and Potentials, 228th American Chemical Society National Meeting, Philadelphia, Pennsylvania. (August 22-26, 2004)

     
     

    "Messing Around with Microbial Metabolism." Department of Biochemistry & Cell Biology, Rice University, Houston, Texas. (August 25, 2003)

     
     

    "Metabolism of Nitroaromatics by Clostridium acetobutylicum." Army Research Office Biosciences Workshop, Cashier, North Carolina. (April 27-30, 2003) With Rudolph, F.B., and Hughes, J.B.

     
     

    "Regulating Cofactors as a Metabolic Engineering Strategy." Society for Industrial Microbiology, Minneapolis, Minnesota. (August 10-14, 2003) With San, K.-Y.

     
     

    "Efforts in Metabolic Engineering of Microbes: Escherichia coli and Clostridium acetobutylicum." Chemistry-Biology Interface Program, University of Delaware, Newart, Delaware. (May 7, 2002)

     
     Keynote Speaker
     

    "Manipulation and balance of reducing equivalents to enhance productivity of chemicals in E. coli." Institute of Biological Engineering 2008 Annual Conference, CIII Advances in Engineering Metabolism & Microbial Conversion, Chapel Hill, North Carolina. (March 6-9, 2008) With K.-Y. San

     
     

    "Microbial Production of Butanol." 2nd North American Symposium on Chemical Reaction Engineering NASCRE-2, Houston, Texas. (February 4-7, 2007)

     
     Lectures
     

    "Metabolic engineering of succinate production in Escherichia coli." Cathay Industrial Biotech, Ltd, Shanghai, China. (October 17, 2008)

     
     

    "Pathway design for biochemical production in Escherichia coli." Society of Industrial Microbiology (SIM) Annual Meeting, San Diego, California. (August 10-14, 2008)

     
     

    "Strategies to enhance recombinant protein production in Escherichia coli by reducing acetate accumulation." Society of Industrial Microbiology (SIM) Annual Meeting, San Diego, California. (August 10-14, 2008)

     
     Other
     

    "Improvement of Production of Biochemicals through Metabolic Engineering." SIM Annual Meeting, Denver, Colorado. (July 29-August 2, 2007) With San, K.-Y.

     
     

    "Increasing Intracellular Reducing Equivalents to Enhance Productivity in Escherichia coli." Third Conference on Renewable Resources and Biorefineries, Ghent, Belgium. (June 4-6, 2007) With San, K.-Y.

     
     

    "Value-added Chemicals from Grain Sorghum: Isoamyl acetate and Succinic Acid Biosynthesis via Fermentation and Engineered Escherichia coli." AACC International Annual Meeting, San Antonio, Texas. (October 7-10, 2007) With Vadlani, P.V, and San, K.-Y.

     
     

    "Analysis of Escherichia coli Global Redox Sensing/Regulation Networks and Cellular Responses." Israel Society for Microbiology 2006 Annual Meeting, Beer Sheva, Israel (S.S. Levanon, J. Zhu, K.-Y. San and G.N. Bennett). (February 21-22, 2006)

     
     

    "Design and Analysis of Highly Efficient Escherichia coli Succinate Production Systems." 62nd Southwest Regional Meeting of The American Chemical Society, Houston, Texas (K.Y. San and G.N. Bennett). (October 19-22, 2006)

     
     

    "Alternative 2D Visualization for Differential Expression Analysis." Beyond Genomics 2001 (Proteomics section), San Francisco, California. (June 2001) With with D. Allen, M. Hurley, R. Edmondson, M. Pisano, and B. Allen

     
     

    "Application of Metabolic and Biochemical Engineering Techniques for the Enhancement of Metabolic Flux Throughput in Escherichia coli." Nineteenth Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February 8-9, 2001) With with A. Chhiber and K.-Y. San

     
     

    "Cofactor Engineering of Acetyl-CoA and Its Subsequence Effect on the Production of Ester in Escherichia coli." Nineteenth Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February 8-9, 2001) With with R.V. Vadali, E. Horton, K.-Y. San, and F.B. Rudolph

     
     

    "Cofactor Engineering of Acetyl-CoA and its Subsequence Effect on Metabolic Flux Redistribution in Escherichia coli." 2001 Annual AIChE Meeting, Nevada. (November 4-9, 2001) With with R.V. Vadali, E. Horton, K.-Y. San and F.B. Rudolph

     
     

    "Degradation of TNT by Clostridium acetobutylicum." ARO-Biology Workshop, Cashiers, North Carolina. (April 29-May 2, 2001) With with J.B. Hughes and F.B. Rudolph

     
     

    "Effect of Different Carbon Sources on the Succinate Production in Escherichia coli with Overexpression of the Feedback Resistant Sorghum PEPC System." 2001 Annual AIChE Meeting, Nevada. (November 4-9, 2001) With with H. Lin, S.G. Patel, A. Chhiber and K.-Y. San

     
     

    "Expression Analysis of the Eschericia coli Genome During Low and High Cell Density Growth in Batch and Chemostat Modes." Nineteenth Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February 8-9, 2001) With with Y.-T. Yang and K.-Y. San

     
     

    "Expression of Yeast Alcohol Acetyltransferase Genes in Escherichia coli and Clostridium acetobutylicum for the Production of Esters." American Society for Microbiology National Meeting, Orlando, Florida. (May 20-24, 2001) With with C.E. Horton and F. Rudolph

     
     

    "The Effect of Genetic Manipulations on the NAD(H+) Ratio and the Metabolic Flux Redistribution in Escherichia coli." Nineteenth Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February 8-9, 2001) With with S.J. Berrios-Rivera and K.-Y. San

     
     

    "The Effect of Manipulating NADH Availability on the Distribution of Metabolites in Escherichia coli." 2001 Annual AIChE Meeting, Nevada. (November 4-9, 2001) With with S.J. Berrios-Rivera and K.-Y. San

     
     

    "Cloning Sequencing and Characterization of the Gene Encoding Flagellin, flaC and the Posttranslational Modification of the Flagellin from Clostridium acetobutylicum ATCC 824." Clostridium 2000, The Sixth International Workshop on the Regulation of Metabolism, Genetics and Development of the Solvent- and Acid-Forming Clostridia, Urbana, Illinois. (May 26-27, 2000) With M. Lyristis, Z.L. Boynton, D. Petersen, Z. Kan and F.B. Rudolph

     
     

    "Expression of the Klebsiella pneumoniae CG21 Acetoin Reductase Gene in Clostridium acetobutylicum ATCC 824." Clostridium 2000, The Sixth International Workshop on the Regulation of Metabolism, Genetics and Development of the Solvent- and Acid-Forming Clostridia, Urbana, Illinois. (May 26-27, 2000) With S. Wardwell, Y.T. Yang and H.Y. Chang

     
     

    "Expressional Analysis of Escherichia coli During Low and High Cell Density Growth." Metabolic Engineering III, Colorado Springs, Colorado. (October 22-27, 2000) With Y.-T. Yang and K.-Y. San

     
     

    "Expressional Analysis of Escherichia coli Genome During Low and High Cell Density Growth." Eighteenth Annual Houston Conference on Biomedical Engineering Research, Houston, Texas. (February 10-11, 2000) With Y.-T. Yang and K.-Y. San

     
     

    "Metabolic Engineering Through Cofactor Manipulation and Its Effects on Metabolic Flux Redistribution in E. coli." Metabolic Engineering III, Colorado Springs, Colorado. (October 22-27, 2000) With K.-Y. San, S. Berrios-Rivera, E. Horton, and R.V. Vadali

     
     

    "Strategies for Metabolic Engineering of Clostridium acetobutylicum." 10th National American Society of Microbiology Meeting, Los Angeles, California. (March 25, 2000)

     
     

    "The Effect of NADH Manipulations of the NADH/NAD+ Ratio and Metabolic Flux Redistribution in Escherichia coli." Eighteenth Annual Houston Conference on Biomedical Engineering Research, Houston, Texas. (February 10-12, 2000) With S.J. Berrios-Rivera and K.-Y. San

     
     

    "Cloning, Sequencing and Characterization of the Gene Encoding Flagellin, flaC, and the Post-translational Modification of the Flagellin from Clostridium acetobutylicum ATCC 824." 99th National American Society for Microbiol. Meeting, Chicago, Illinois. (June 2, 1999) With with Lyristis, M., Boynton, Z.L., Petersen, D., Kan, Z., and Rudolph, F.B.

     
     

    "Effect of Glucose Analog Supplementation in Anaerobic Chemostat Cultures of E. coli." 17th Annual Houston Conference on Biomedical Engineering Research, Houston, Texas. (February 11-12, 19999) With with Berrios-Rivera, S.J., Yang, Y.-T., and San, K.-Y.

     
     

    "Expression of the Klebsiella pneuomoneae CG21 Acetoin Reductase Gene in Clostridium acetobutylicum ATCC 824." 99th National American Society for Microbiol. Meeting, Chicago, Illinois. (June 1, 1999) With with Wardwell, S.A., Yang, Y.T., and Chang, H.Y.

     
     

    "Improved Electrotransformation Method for Clostridium acetobutylicum ATCC 824." 99th National American Society for Microbiol. Meeting, Chicago, Illinois. (June 3, 1999) With with Tyurin, M., Padda, R.S., Huang, K., Wardwell, S.A.M., and Caprette, D.

     
     

    "Reduction of Nitroaromatic Explosives by Clostridium acetobutylicum." The Fifth International Symposium "In Situ and On-site Bioremediation, San Diego, California. (April 19-22, 1999) With with Hughes, J.B., Wang, C., Zhang, C., Padda, R.S., and Clark, S.

     
     

    "The Effect of nuo and ackA-pta Deletion on the Redistribition of Metabolic Fluxes in Escherichia coli." 17th Annual Houston Conference on Biomedical Engineering Research, Houston, Texas. (February 11-12, 1999) With with Yang, Y.-T., and San, K.-Y.

     
     

    "The Effect of Inactivation of neo and ackA-pta on the Expression of the pfl (pyruvate formate-lyase) Gene in Escherichia coli." Annual Meeting of the American Institute of Chemical Engineers, Dallas, Texas. (October 31-November 5, 1999)

     
     

    "The Effect of NAD(H/+) Perturbations on the NADH/NAD+ Ratio and Metabolic Flux Redistribution of E. coli." Annual Meeting of American Institute of Chemical Engineers, Dallas, Texas. (October 31-November 5, 1999) With with Berrios-Rivera, S.J., Lu, B., and San, K.-Y.

     
     Posters
     

    "Precise and scarless genome modification in Escherichia  coli using transposase." Society for Industrial Microbiology and Biotechnology Annual Meeting, Saint Louis, MO. (July 20-24, 2014) With Chandresh Thakker

     
     

    "The Rice University Biological networks REU: a site for students pursuing diverse science and engineering majors." NSF REU Grantees Meeting, Washington, DC. (April 14-15, 2014) With Jonathan Silberg

     
     

    "Cottonseed meal derived soluble sugars as an economical feedstock for the fermentative production of C4 dicarboxylic acid using engineered E. coli  ." Society for Industrial Microbiology and Biotechnology Annual Meeting, San Diego, CA. (August 11-15, 2013) With Chandresh Thakker, and Ka-Yiu San

     
     

    "Metabolic engineering of E. coli to minimize byproduct formate during succinate production by heterologous overexpression of NAD+-dependent formate dehydrogenase." Society for Industrial Microbiology and Biotechnology Annual Meeting, San Diego, CA. (August 11-15, 2013) With Grant J. Balzer, Chandresh Thakker, and Ka-Yiu San

     
     

    "A Metabolic Engineering Approach to Improve NADPH Bioavailability in E. coli." 60th Meeting of the Society of Industrial Microbiology, San Francisco, CA . (August) With Yipeng Wang & Ka-Yiu San

     
     

    "Co-production of riboflavin and biobutanol in Clostridium acetobutylicum." Clostridium 10, Wageningen, The Netherlands. (September 29-October 1, 2008) With X. Cai

     
     

    "Co-production of riboflavin and biobutanol in Clostridium acetobutylicum." 30th Symposium on Biotechnology for Fuels and Chemicals, New Orleans, Louisiana. (May 4-7, 2008) With X. Cai

     
     

    "Co-production of riboflavin and biobutanol in Clostridium acetobutylicum." Genes to Products- Agricultural Plant, Microbe, and Biobased Product Research, Bethesda, Maryland. (April 16-18, 2008) With X. Cai

     
     

    "Construction and analysis of cell population heterogeneity of negative feedback gene auto-regulatory systems in Escherichia coli." AIChE Meeting, Philadelphia, Pennsylvania. (November 12-16, 2008) With M. Li, J. Zhu, R. Simonette, M.L. Harrison, S. Portle and K.-H. San

     
     

    "Detection and analysis of Clostridium acetobutylicum in soils." Clostridium 10, Wageningen, The Netherlands. (September 29-October 1, 2008) With P. Sar and R. Kutty

     
     

    "Manipulation and balance of reducing equivalents to enhance productivity of chemicals in E. coli." Synthetic Biology 4.0, Hong Kong. (October 10-12, 2008) With K.-Y. San

     
     

    "Production of C4 compounds by metabolic engineered Escherichia coli." AIChE Meeting, Philadelphia, Pennsylvania. (November 12-16, 2008) With I. Martinez, A. Lee and K.-Y. San

     
     

    "Production of natural flavor chemicals: Isoamyl acetate biosynthesis from renewable sugars using engineered Escherichia coli." AIChE Meeting, Philadelphia, Pennsylvania. (November 12-16, 2008) With P.V. Vanlani and K.-Y. San

     
     

    "Production of platform chemicals from renewable sugars: Low-cost succinic acid fermentation using engineered Escherichia coli." AIChE Meeting, Philadelphia, Pennsylvania. (November 12-16, 2008) With P.V. Vadlani and K.-Y. San

     
     

    "Visualizing the results of metabolic pathway queries." Graph Drawing 2008, 16th International Symposium on Graph Drawing, Crete, Greece. (September 21-24, 2008) With A.P. Heath and L.E. Kavraki

     
     

    "Biological Aspects of Deciphering and Engineering Regulatory Networks." Joint Meeting of DOE Genomics to Life: Systems Biology for Energy and Environment and USDA-DOE Plant Feedstock Genomics for Bioenergy Awardee Workshop 2007 and Metabolic Engineering Working Group Interagency Conference on Metabolic Engineering 2007, Bethesda, Maryland. (February 11-14, 2007)

     
     

    "Characterization of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) Production in Escherichia coli." Twenty Fourth Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February 8-9, 2007) With Wong, M.S., Causey, T.S., Mantzaris, N., and San, K.-Y.

     
     

    "Complex Inducer-Controlled Behavior In Cell Populations With Oscillatory Genetic Network Dynamics." 2007 Annual AIChE meeting, Salt Lake City, Utah. (November 4-9, 2007) With Portle, S., San, K.-Y., and Mantzaris, N.V.

     
     

    "Computational Modeling and Metabolic Analysis of Succinate Production in Engineered Escherichia coli." 29th Symposium on Biotechnology for Fuels and Chemicals, Denver, Colorado. (April 29-May 2, 2007) With Sánchez, A.M., Lin, H., Cox, S.J., Shalel Levanon, S., Peercy, B., San, K.-Y.

     
     

    "Construction And Characterization Of Negative Feedback Gene Regulation Systems In Escherichia coli." 2007 Annual AIChE meeting, Salt Lake City, Utah. (November 4-9, 2007) With Li, M., Zhu, J., Mantzaris, N.V., and San, K.-Y.

     
     

    "Development and Evaluatation of a Q-PCR Based Approach for Accurate Quantification of the Hydrogenase Gene from Clostridium acetobutylicum to Monitor TNT Bioremediation." 3rd International Conference on Environmental Science and Technology (EST2007), Houston, Texas. (August 6-9, 2007) With Kutty, R.

     
     

    "Increasing The In Vivo Availability Of Reducing Equivalents By Metabolic Engineering In Escherichia coli." 2007 Annual AIChE meeting, Salt Lake City, Utah. (November 4-9, 2007) With Martinez, I., Zhu, J., Lin, H., and San, K.-Y.

     
     

    "Manipulation of NADH Availability in Escherichia coli by Genetic Modification of the Glycolytic Pathway." , Twenty Fourth Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February 8-9, 2007) With Martinez, I., Zhu, J., Lin, H., San, K.-Y.

     
     

    "Metabolic Engineering of Escherichia coli to Overproduce C4 Compounds." 2007 Annual AIChE meeting, Salt Lake City, Utah. (November 4-9, 2007) With Lee, A., Martinez, I., San, K.-Y.

     
     

    "Metabolic Engineering of Pyruvate Metabolism By Combining Pathway Mutations And Manipulating Nadh Availability." 2007 Annual AIChE meeting, Salt Lake City, Utah. (November 4-9, 2007) With Zhu, J., and San, K.-Y.

     
     

    "Microbial Production of Butanol." 2nd North American Symposium on Chemical Reaction Engineering, NASCRE-2, Houston, Texas. (February 4-7, 2007)

     
     

    "Reduction of Acetate Production In Escherichia Coli for Increased Recombinant Protein Production." 2007 Annual AIChE meeting, Salt Lake City, Utah. (November 4-9, 2007) With Wong, M.S., Wu, S., Causey, T.B., and San, K.-Y.

     
     

    "User-Controlled Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics." Twenty Fourth Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February 8-9, 2007) With Portle, S., San, K.-Y., and Mantzaris, N.

     
     

    "Analysis of Escherichia coli Global Redox Sensing/Regulation Networks and Cellular Responses." Israel Society for Microbiology 2006 Annual Meeting, Beer Sheva, Israel. (February 21-22, 2006) With S. Shaele Levanon, J. Zhu and K.-Y. San

     
     

    "Biosynthesis of Ethyl Esters in Recombinant Escherichia coli Expressing Alcohol Acyltransferase from Strawberry and Thiolase from Clostridium acetobutylicum." 106th ASM General Meeting, Orlando, Florida. (May 21-25, 2006) With Y.-C. Park, L. Sullivan and K.-Y. San

     
     

    "Construction of a Genetic Toggle Switch for Polyhydroxyalkanoate Production in Escherichia coli." 106th ASM General Meeting, Orlando, Florida. (May 21-25, 2006) With T.B. Causey, S. Portal, N. Mantzaris, and K.-Y. San

     
     

    "Development of a Mechanistic Model for Sugar-Utilization Regulatory Systems." 2006 Annual AIChE Meeting, San Francisco, California. (November 12-17, 2006) With J. Zhu, R. Peacock, J.V. Shanks R. Gonzalez and K.-Y. San

     
     

    "Effect of yfiD and poxB Gene Distruption on Microaerobic Pyruvate Catabolization and Metabolic Flux Distributions Based on C-13 Labeling Experiments." 2006 Annual AIChE Meeting, San Francisco, California. (November 12-17, 2006) With J. Zhu, S. Shalel Levanon and K.-Y. San

     
     

    "Effect of Operating Conditions on the Performance of Efficient Succinate-Producing Escherichia coli Strains." 2006 Annual AIChE Meeting, San Francisco, California. (November 12-17, 2006) With I. Martinez and K.-Y. San

     
     

    "Establishment of Q-PCR Based Approach for Accurate Quantification of Hydrogenase Gene from Clostridium acetobutylicum to Monitor TNT Bioremediation." Clostridium IX: Ninth International Workshop on the Regulation of Metabolism, Genetics and Development of the Solvent- and Acid-forming Clostridia, Houston, Texas. (May 18-21, 2006) With R. Kutty

     
     

    "Genetic Modification of NADPH Availability in Escherichia coli." 3rd International E. coli Alliance Conference on Systems Biology (ICA2006), Juju, Korea. (October 31-November 4, 2006) With I. Martinez, J. Zhu, H. Lin and K.-Y. San

     
     

    "Genetic Modification of NADPH Availability in Escherichia coli." Natural Products Discovery and Production: New Challenges, New Opportunities, Santa Fe, New Mexico. (June 4-8, 2006) With I. Martinez, J. Zhu, H. Lin and K.-Y. San

     
     

    "Increasing the in vivo Nadph Availability by Metabolic Engineering in Escherichia coli." 2006 Annual AIChE Meeting, San Francisco, California. (November 12-17, 2006) With I. Martinez, J. Zhu, H. Lin and K.-Y. San

     
     

    "Mathematical and Metabolic Analysis of Succinate Production in Engineered Escherichia coli." 3rd International E. coli Alliance Conference on Systems Biology (ICA2006), Juju, Korea. (October 31-November 4, 2006) With A.M. Sanchez, H. Lind, S.J. Cox, S. Shalel Levanon, B. Peercy and K.-Y. San

     
     

    "Purification and Characterization of Ferredoxin with N-terminal Extension from Clostridium acetobutylicum ACCT 824." 106th ASM General Meeting, Orlando, Florida. (May 21-25, 2006) With R. Kutty

     
     

    "Research on Genetic Technology in Clostridium acetobutylicum." Clostridium IX: Ninth International Workshop on the Regulation of Metabolism, Genetics and Development of the Solvent- and Acid-forming Clostridia, Houston, Texas. (May 18-21, 2006) With L. Sullivan, M. Scotcher, Y. Zhao and M. Tyurin

     
     

    "The Clostridial Hydrophobic with a Conserved W Family (ChW) of Proteins Unique to Clostridium acetobutylicum." 106th ASM General Meeting, Orlando, Florida. (May 21-25, 2006) With L. Sullivan

     
     

    "The Clostridial Hydrophobic with a Conserved W Family (ChW) of Proteins Unique to Clostridium acetobutylicum." Clostridium IX: Ninth International Workshop on the Regulation of Metabolism, Genetics and Development of the Solvent- and Acid-forming Clostridia, Houston, Texas. (May 18-21, 2006)

     
     

    "User-Controlled Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics." 2006 Annual AIChE Meeting, San Francisco, California. (November 12-17, 2006) With S. Portle, T.B. Causey, K.-Y. San and N.V. Mantzaris

     
     

    "Utilization of a Genetic Toggle in the Control of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) Composition in Recombinant Escherichia coli." 2006 Annual AIChE Meeting, San Francisco, California. (November 12-17, 2006) With M. Wong, T.B. Causey, N.V. Mantzaris and K.-Y. San

     
     

    "Batch Reactor Design and Optimization for Large-scale Succinate Production under Absolute Aerobic Conditions Using a Metabolically Engineered Aerobic Succinate Production System in Escherichia coli." Twenty-second Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February 10-11, 2005) With H. Lin and K.-Y. San

     
     

    "Characterization of Trinitrotoluene Transforming Flavoprotein from Clostridium acetobutylicum." General Meeting, American Society of Microbiology, Atlanta, Georgia. (June 5-9, 2005) With R. Kutty

     
     

    "Characterization of a Gene-Switching Network." Twenty-second Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February 10-11, 2005) With S. Portle, M. Harrison, K.-Y. San and N. Mantzaris

     
     

    "Chemostat Culture Characterization of Metabolically Engineered Escherichia coli Mutant Strains Designed for Optimal Aerobic Production of Succinate." ACS National Meeting, San Diego, California. (March 2005) With H. Lin and K.-Y. San

     
     

    "Construction of a Genetic Toggle Switch for the Production of Polyhydroxyalkanoates in Escherichia coli." ACS National Meeting, San Diego, California. (March 2005) With T.B. Causey, S. Portle and K.-Y. San

     
     

    "Construction of a Genetic Toggle Switch for Polyhydroxyalkanoate Production in Escherichia coli." Annual AIChE Meeting, Cincinnati, Ohio. (October 30-November 4, 2005) With T.B. Causey, S. Portle, K.-Y. San and N.V. Mantzaris

     
     

    "Development of Efficient Escherichia coliSuccinate Production Systems." Biochemical Engineeringt XIV, Harrison Hot Springs, BC., Canada. (July 10-14, 2005) With K.-Y. San, A.M. Sanchez and H. Lin

     
     

    "Dynamics of Cell Populations Carrying Gene-Switching Networks with Fluorescent Protein Markers of Different Half-Lives." Annual AIChE Meeting, Cincinnati, Ohio. (October 30-November 4, 2005) With S. Portle, T.B. Causey, K.-Y. San and N.V. Mantzaris

     
     

    "Effect of Acetyl-CoA Synthetase Overexpression in Escherichia coli on Metabolite Production in the Presence of Glucose and Acetate Carbon Sources." ACS National Meeting, San Diego, California. (March 2005) With H. Lin, N.M. Castro and K.Y. San

     
     

    "Effect of Gene Inactivation of adhE in pta-ackA in NADH Dehydrogenase-deficient Escherichia coli on Anaerobic Metabolic Pathway." Annual Meeting and International Symposium of the Korean Society for Microbiology and Biotechnology, Seoul, Korea. (June 20-July 1, 2005) With N.R. Yun and K.-Y. San

     
     

    "Effect of Gene Inactivation of arcA and/or fnr in Escherichia coli on Anaerobic Metabolic Pathway." International Meeting of the Microbiological Society of Korea, Whengsun, Kangwon-do, Korea. (May 12-13, 2005) With N.R. Yun and K.-Y. San

     
     

    "Effect of Oxygen on the E. coli Global Redox Sensing/Regulation Networks and Cellular Responses." ACS National Meeting, San Diego, California. (March 2005) With S. Shalel Levanon and K.-Y. San

     
     

    "Effect of Oxygen on the E. coli Global Redox Sensing/Regulation Networks and Metabolic Flux Distribution Based on C-13 Labeling Experiments." Annual AIChE Meeting, Cincinnati, Ohio. (October 30-November 4, 2005) With J. Zhu, K.-Y. San, S. Shalel Lenanon and B. Peercy

     
     

    "Effect of Oxygen on the Escherichia coli Global Redox Sensing/Regulation Networks and Cellular Responses." Twenty-second Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February 10-11, 2005) With S.S. Levanon and K.-Y. San

     
     

    "Mathematical Model of Oxygen Sensing in Eschericia coli." Twenty-second Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February 10-11, 2005) With B. Peercy, S.S. Levanon, S. Cox and K.-Y. San

     
     

    "Metabolic Network Engineering and Optimization of the Aerobic Central Metabolism of Escherichia coli for Achieving the Maximum Theoretical Yield of Succinate under Absolute Aerobic Conditions." Twenty-second Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February 10-11, 2005) With H. Lin and K.-Y. San

     
     

    "Population Distribution Patterns in Cells with Oscillatory Genetic Network Dynamics." Annual AIChE Meeting, Cincinnati, Ohio. (October 30-November 4, 2005) With S. Portle, M.L. Harrison, K.-Y. San and N.V. Mantzaris

     
     

    "Role of nuo Gene Cluster during Anaerobic Pyruvate Metabolism in Escherichia coli." Twenty-second Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February 10-11, 2005) With R. Singh and K.-Y. San

     
     

    "Role of nuo Gene Cluster during Anaerobic Pyruvate Metabolism in Escherichia coli." ACS National Meeting, San Diego, California. (March 2005) With R. Singh and K.-Y. San

     
     

    "The Clostridial Hydrophobic with a Conserved W Family (ChW) of Proteins Unique to Clostridium acetobutylicumL Analysis of Promoter Architecture and Expression of Two of its Members, chw14 and chw16." Lost Pines Molecular Biology Conference, Smithville, Texas. (October 21-23, 2005) With L. Sullivan

     
     

    "A genetic network driven metabolic flux analysis scheme." Metabolic Engineering Conference, Lake Tahoe, California. (September 19-23, 2004) With K.-H. San, S. Shalel Levanon, S. Cox, B. Peercy, J.V. Shanks and R. Gonzalez

     
     

    "A metabolic engineering application: Pathway design and engineering for enhancing succinate productivity in both aerobic and anaerobic fermentation processes." Metabolic Engineering Conference, Lake Tahoe, California. (September 19-23, 2004) With H, Lin, A. Sanchez and K.Y. San

     
     

    "A practical metabolic network design and optimization framework to guide pathway reconstruction." Annual National AIChE Meeting, Austin, Texas. (November 7-12, 2004) With S. Cox, S. Shalel Levanon, H. Lin, A.M. Sanchez and K.-Y. San

     
     

    "Batch reactor design and optimization for large-scale succinate production under absolute aerobic conditions using a metabolically engineered aerobic succinate production system in Escherichia coli." Annual National AIChE Meeting, Austin, Texas. (November 7-12, 2004) With H. Lin and K.-Y. San

     
     

    "Characterization of a metabolically engineered Escherichia coli mutant designed for aerobic production of succinate: A systematic approach to study the metabolic response to multiple pathway manipulations in the central metabolism." 227th American Chemical Society National Meeting, Anaheim, California. (March 28-April 1, 2004) With H. Lin and K.-Y. San

     
     

    "Cloning and expression of the gene encoding the Clostridium acetobutylicum (ATCC 824) thermostable xylanase 10A1 in E. coli." International Workshop on the Regulation of Metabolism, Genetics and Development of the Solvent- and Acid-forming Clostridia, Edinburgh, Scotland. (July 1-4, 2004) With M.K. Ali and F.B. Rudolph

     
     

    "Cloning expression and characterization of an NADH:flavin mononucleotide nitroreductase nitA from Clostridium acetobutylicum." 2004 Texas Branch Meeting of The American Society for Microbiology, Houston, Texas. (November 4-6, 2004) With R. Kutty

     
     

    "Co-factor engineering as a metabolic engineering tool to increase process productivity." Metabolic Engineering Conference, Lake Tahoe, California. (September 19-23, 2004) With K.-Y. San, R. Vadali, H. Lin and A. Sanchez

     
     

    "Construction and characterization of a gene-switching network." Annual National AIChE Meeting, Austin, Texas. (November 7-12, 2004) With S. Portle, M. Harrison, K.-Y. San and N. Mantzaris

     
     

    "Development of a metabolic network design and optimization framework incorporating implementation constraints." 227th American Chemical Society National Meeting, Anaheim, California. (March 28-April 1, 2004) With K.-H. San, S. Shalel Levanon, H. Lin and S. Cox

     
     

    "Development of high yield Escherichia coli succinate production systems through pathway restruction." Annual National AIChE Meeting, Austin, Texas. (November 7-12, 2004) With A.M. Sanchez and K.-Y. San

     
     

    "Effect of oxygen on the E. coli global redox sensing/regulation networks and cellular responses." 227th American Chemical Society National Meeting, Anaheim, California. (March 28-April 1, 2004) With S. Shalel Levanon and K.-Y. San

     
     

    "Effect of oxygen on the E. coli global redox sensing/regulation networks and cellular responses." 2004 Texas Branch Meeting of The American Society for Microbiology, Houston, Texas. (November 4-6, 2004) With S. Shalel Levanon and K.-Y. San

     
     

    "Effect of oxygen on the E. coli global redox sensing/regulation networks and cellular responses." Annual National AIChE Meeting, Austin, Texas. (November 7-12, 2004) With S. Shalel Levanon and K.-Y. San.

     
     

    "Function of E. coli global redox sensor/ regulator, ArcAB, in anaerobic metabolic regulation and cellular responses." American Society for Microbiology (ASM) 104th General Meeting, New Orleans, Louisiana. (May 23-27, 2004) With N.-R. Yun and K.-Y. San

     
     

    "Function of redox proteins, Ndh and Nuo, in anaerobic metabolic regulation and cellular responses in E. coli." American Society for Microbiology Conference on Integrating Metabolism and Genomics, Montreal, Quebec, Canada. (April 30-May 3, 2004) With N.-R. Yun and K.-Y. San

     
     

    "Genetic reconstruction of the tricarboxylic acid cycle in Escherichia coli for the complete aerobic production of succinate through the glyoxylate bypass." 227th American Chemical Society National Meeting, Anaheim, California. (March 28-April 1, 2004) With H. Lin and K.-Y. San

     
     

    "Metabolic engineering of Escherichia coli to study the effects of ackA-pta and poxB deletions on the control of acetate production." 227th American Chemical Society National Meeting, Anaheim, California. (March 27-April 1, 2004) With C.R. Dittrich and K.-Y. San

     
     

    "Metabolic engineering of NADPH availability on the productivity of poly(3-hydroxybutyrate) in Escherichia coli." 227th American Chemical Society National Meeting, Anaheim, California. (March 28-April 1, 2004) With A.M. Sanchez, I. Hussain and K.-Y. San

     
     

    "Metabolic engineering of the central metabolic pathways of Escherichia coli: A systematic approach to study the metabolic response to multiple pathway manipulations for the production of fermentation products." Twenty First Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February12-13, 2004) With H. Lin and K.-Y. San

     
     

    "Metabolic network engineering and optimization of the aerobic central metabolism of Escherichia coli for achieving the maximum theoretical yield of succinate under absolute aerobic conditions." Annual National AIChE Meeting, Austin, Texas. (November 7-12, 2004) With H. Lin and K.-Y. San

     
     

    "Modeling of the oxygen sensing/regulatory circuit in E. coli." 227th American Chemical Society National Meeting, Anaheim, California. (March 28-April 1, 2004) With K.-H. San, S. Shalel Levanon and S. Cox

     
     

    "Oxygen sensing and regulation of cytochromes in E. coli." Gordon Research Conference on Theoretical Biology and Biomathematics, Tilton, New Hampshire. (June 6-11, 2004) With B. Peercy, S. Shalel Levanon, S.J. Cox and K.-Y. San

     
     

    "Pathway engineering of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity." 227th American Chemical Society National Meeting, Anaheim, California. (March 28-April 1, 2004) With A.M. Sanchez and K.-Y. San

     
     

    "Pathway engineering of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity." Twenty First Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February 12-12, 2004) With A.M. Sanchez and K.-Y. San

     
     

    "Production of isoamyl acetate in E. coli mutant strains with deletion of the ackA-pta and poxB pathways." Annual National AIChE Meeting, Austin, Texas. (November 7-12, 2004) With C.R. Dittrich and K.-Y. San

     
     

    "Redistribution of metabolic fluxes in the central aerobic metabolic pathway of E. coli mutant strains with deletion of the ackA-pta and poxB pathways." Twenty First Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February 12-13, 2004) With C.R. Dittrich, R.V. Vadali, A.M. Sanchez, and K.Y. San

     
     

    "Role of nuo gene cluster during anaerobic pyruvate metabolism in Escherichia coli." Annual National AIChE Meeting, Austin, Texas. (November 7-12, 2004) With R. Singh and K.-Y. San

     
     

    "SpoIIE regulates sporulation but does not directly affect solventogenesis in Clostridium acetobutylicum ATCC824." 2004 Texas Branch Meeting of The American Society for Microbiology, Houston, Texas. (November 4-6, 2004) With M.D. Scotcher

     
     

    "The expression and regulation of ChW 14 and 16: possibly related to solvent transition in Clostridium acetobutylicum." 2004 Texas Branch Meeting of The American Society for Microbiology, Houston, Texas. (November 4-6, 2004) With L. Sullivan

     
     

    "The lysine riboswitch: A model for riboswitch control of metabolite synthesis and transport in clostridia." Clostridium VIII, Eighth International Workshop on the Regulation of Metabolism, Genetics and Development of the Solvent- and Acid-forming Clostridia, Edinburgh, Scotland. (July 1-4, 2004) With M. Scotcher

     
     

    "Transformation of 2,4,6-trinitrotoluene by Fe-hydrogenase from Clostridium acetobutylicum ATCC 824: Relevance to mechanism of catalysis." International Workshop on the Regulation of Metabolism, Genetics and Development of the Solvent- and Acid-forming Clostridia, Edinburgh, Scotland. (July 1-4, 2004) With R. Kutty and F.B. Rudolph

     
     

    "Transformation of 2,4,6-trinitrotoluene by Fe-hydrogenase from Clostridium acetobutylicum: Relevance to mechanism of catalysis." The Fourth International Conference on Remediation of Chlorinated and Recalcitrant Compounds, Monterey, California. (May 2004) With R. Kutty and F.B. Rudolph

     
     

    "Two stage fermentations using high yield succinate producing Escherichia coli systems." Annual National AIChE Meeting, Austin, Texas. (November 7-12, 2004) With A.M. Sanchez and K.-Y. San

     
     

    "A Data Driven Mathematical Model of the O2 Sensing/Regulatory Circuit in E. coli." 2003 Annual AIChE Meeting, San Francisco, California. (November 16-21, 2003) With S. Shalel Levanon, K.-Y. San and S. Cox

     
     

    "Analysis of E. coli Global Redox Sensing/Regulation Networks and Cellular Responses." 2003 Annual AIChE Meeting, San Francisco, California. (November 16-21, 2003) With S. Shalel Levanon and K.-Y. San

     
     

    "Characterization of a Thermostable Xyn10A Enzyme from Mesophilic Clostridium acetobutylicum Strain ATCC 824." American Society for Microbiology National Meeting, Washington, D.C.. (May 2003) With Ali, M.K. and Rudolph, F.B.

     
     

    "Cloning and Expression of the Gene Encoding the Clostridium acetobutylicum (ATCC 824) Thermostable Xylanase10A1 in E. coli." Cellulose and Cellulosome Gordon Research, Andover, New Hampshire. (July 27-August 1, 2003) With Ali, M.K., and Rudolph, F.B.

     
     

    "Cofactor Engineering of Acetyl-CoA and its Subsequent Effect on Metabolic Flux Redistribution and the Production of Ester in Escherichia coli." Twentieth Conference on Biomedical Engineering Research in Houston, Houston, Texas. (April 3-4, 2003) With R.V. Vadali, E. Horton, K.-Y. San and F.B. Rudolph

     
     

    "Cofactor Engineering of CoA/Acetyl-CoA: A Novel Metabolic Engineering Technique to Enhance Process Productvity in Escherichia coli." 2003 Annual AIChE Meeting, San Francisco, California. (November 16-21, 2003) With R.V. Vadali and K.-Y. San

     
     

    "Cofactor Metabolic Engineering: Comparative Study of the Effect of Increasing NADH Availability in E. coli Chemostat Cultures on Minimal and Complex Medium." 225th American Chemical Society National Meeting, New Orleans, Louisiana. (March 23-27, 2003) With A. Sanchez and K.-Y. San

     
     

    "Cofactor and Pathway Engineering: A Precise Strategy to Increase Productivity of Industrially Useful Compounds in Escherichia coli." 2003 Annual AIChE Meeting, San Francisco, California. (November 16-21, 2003) With A.M. Sanchez and K.-Y. San

     
     

    "Effect of Increased NADH Regeneration on Succinate Production in Escherichia coli Using NAD+-Dependent Formate Dehydrogenase." Twentieth Conference on Biomedical Engineering Research in Houston, Houston, Texas. (April 3-4, 2003) With A. Sanchez, H. Lin and K.-Y. San

     
     

    "Effect of Pathway Manipulations on the Production of Lycopene in Metabolically Engineered Escherichia coli." 2003 Annual AIChE Meeting, San Francisco, California. (November 16-21, 2003) With R.V. Vadali, Y. Fu and K.-Y. San

     
     

    "Effect of Pathway Manipulations on the Production of Lycopene in Metabolically Engineered Escherichia coli." Twentieth Conference on Biomedical Engineering Research in Houston, Houston, Texas. (April 3-4, 2003) With Y. Fu, R.V. Vadali and K.-Y. San

     
     

    "Effects of NADH/NAD+ and NADPH/NADP+ Cofactor Pair Manipulations in Escherichia coli." 2003 Annual AIChE Meeting, San Francisco, California. (November 16-21, 2003) With A.M. Sanchez, R.V. Vadali and K.-Y. San

     
     

    "Enhanced Isoamyl Acetate Production in Escherichia coli upon Manipulation of the Actyl-CoA Node." 225th American Chemical Society National Meeting, New Orleans, Louisiana. (March 23-27, 2003) With R.V. Vadali and K.-Y. San

     
     

    "Increasing Acetyl-CoA Pool in the Presence of Overexpressed Phosphoenolpyruvate Carboxylase or Pyruvate Carboxylase Enhances Succinate Production in Escherichia coli." 225th American Chemical Society National Meeting, New Orleans, Louisiana. (March 23-27, 2003) With H. Lin and K.-Y. San

     
     

    "Manipulation of NADH Availability as a Metabolic Engineering Tool on Cofactor Dependent Production Systems." Twentieth Conference on Biomedical Engineering Research in Houston, Houston, Texas. (April 3-4, 2003) With A. Sanchez and K.-Y. San

     
     

    "Metabolic Engineering of a Succinate-Producing Mutant Escherichia coli Strain to Enhance Succinate Productivity." Twentieth Conference on Biomedical Engineering Research in Houston, Houston, Texas. (April 3-4, 2003) With H. Lin and K.-Y. San

     
     

    "Metabolic Engineering of the Central metabolic Pathways of Escherichia coli: A Systematic Approach to Study the Metabolic Response to Multiple Pathway Manipulations for the Production of Fermentation Products." 2003 Annual AIChE Meeting, San Francisco, California. (November 16-21, 2003) With H. Lin and K.-Y. San

     
     

    "Metabolic Flux Analysis Based on Dynamic Genomic Information." Twentieth Conference on Biomedical Engineering Research in Houston, Houston, Texas. (April 3-4, 2003) With S. Cox, S. Levanon and K.-Y. San

     
     

    "Metabolic Flux Analysis Based on Dynamic Genomic Information." 225th American Chemical Society National Meeting, New Orleans, Lousiana. (March 23-27, 2003) With K.-Y. San and S. Cox

     
     

    "Production of Isoamyl Acetate in Genetic Engineered Escherichia coli." Twentieth Conference on Biomedical Engineering Research in Houston, Houston, Texas. (April 3-4, 2003) With R.V. Vadali, E. Horton, K.-Y. San and F.B. Rudolph

     
     

    "Production of Succinate through Metabolic Engineering of Escherichia coli with Coexpression of Sorghum Phosphoenolpyruvate Carboxylase and lactococcus Lactis Pyruvate Carboxylase." Twentieth Conference on Biomedical Engineering Research in Houston, Houston, Texas. (April 3-4, 2003) With H. Lin and K.-Y. San

     
     

    "Redistribution of Metabolic Fluxes in the Central Aerobic Metabolic Pathway of E. coli Mutant Strains with Deletion of the ackA-pta and poxB Pathways." 2003 Annual AIChE Meeting, San Francisco, California. (November 16-21, 2003) With C.R. Dittrich, R.V. Vadali, A.M. Sanchez and K.-Y. San

     
     

    "Spo0A Regulates Global Gene Expression in Clostridium acetobutylicum." American Society for Microbiology National Meeting, Washington, D.C.. (May 2003) With Sullivan, L.

     
     

    "Cofactor Engineering of Acetyl-CoA and its Subsequent Effect on Metabolic Flux Redistribution and the Production of Ester in E. coli." American Institute of Chemical Engineers National Meeting, Indianapolis, Indiana. (November 2002) With Vadali, R.V., San, K.-Y., and Bennett, G.N.

     
     

    "Effect of Different Levels of NADH Availability on Metabolite Distribution in Escherichia coli." American Institute of Chemical Engineers National Meeting, Indianopolis, Indiana. (November 2002) With Berrios-Rivera, S.J., and San, K.-Y.

     
     

    "Effect of Increased NADH Regeneration on Succinate Production in Escherichia coli Using NAD+-Dependent Formate Dehydrogenase." American Institute of Chemical Engineers National Meeting, Indianapolis, Indiana. (November 2002) With Sanchez, A.M., Berrios-Rivera, S.J., Lin, H., Bennett, G.N., and San, K.-Y.

     
     

    "Expression of Alcohol Acetyltransferase in Clostridium acetobutylicum for the Production of Isoamyl Acetate." Clostridium VII Seventh International Workshop on the Regulation of Metabolism, Rostock-Warnemunde, Germany. (Sept. 19-22, 2002) With Horton, C.E., and Rudolph, F.B.

     
     

    "Manipulation of NADH Availability as a Metabolic Engineering Tool on Cofactor Dependent Production Systems." American Institute of Chemical Engineers National Meeting, Indianapolis, Indiana. (November 2002) With Sanchez, A.M., Bennett, G.N., and San, K.-Y.

     
     

    "Production of Isoamyl Acetate in Various Deletion Mutants of Escherichia coli with Overexpression of Yeast ATF2." American Institute of Chemical Engineers National Meeting, Indianapolis, Indiana. (November 2002) With Vadai, R.V., Horton, E., San, K.-Y., and Rudoph, F.B.

     
     

    "Production of Succinate Through Metabolic Engineering of Escherichia coli with Coexpression of Sorghum Phosphoenolpyruvate Carboxylase and Lactococcus Lactis Pyruvate Carboxylase." American Institute of Chemical Engineers National Meeting, Indianapolis, Indiana. (November 2002) With Lin, H., San, K.-Y., and Bennett, G.N.

     
     

    "Studies of the Regulation of Solvent Production Genes in Clostridium acetobutylicum." Clostridium VII Seventh International Workshop on the Regulation of Metabolism, Rostock-Warnemunde, Germany. (Sept. 19-22, 2002) With Scotcher, M., Huang, K-X, Harrison, M.L., and Rudolph, F.B.

     
     

    "The Overexpression of Cyclopropane Fatty Acid Synthase Suppresses Solvent Production in Clostridium acetobutylicum ATCC 824." American Society for Microbiology 102nd General Meeting, Salt Lake City, Utah. (May 19-23, 2002) With Zhao, Y.

     
    Editorial Positions
     Editor, Special issue of Journal Fermentation on Metabolic Engineering . MDPI AG Basel, Switzerland. (2015 - 2015)

     Member of the Editorial Board, Applied Microbiology & Biotechnology. (2010 - 2010)

     Member of the Editorial Board, Recent Patents on Biotechnology. (2011 - 2011)

     Member of the Editorial Board, Electronic Journal of Biotechnology. (2011 - 2011)

     Member of the Editorial Board, Applied Microbiology & Biotechnology. (2011 - 2011)

     Member of the Editorial Board, Recent Patents on Biotechnology. (2010 - 2010)

     Member of the Editorial Board, Electronic Journal of Biotechnology. (2010 - 2010)

     Member of the Editorial Board, Applied Microbiology & Biotechnology. (2007 - 2007)

     Member of the Editorial Board, Electronic Journal of Biotechnology.. (1999 - 1999)

     Member of the Editorial Board, Anaerobe, Applied Microbiology and Biotechnology, Electronic Journal of Biotechnology. (2003 - 2003)

     Member of the Editorial Board, Anaerobe. (2005 - 2005)

     Member of the Editorial Board, Applied Microbiology & Biotechnology. (2006 - 2006)

     Member of the Editorial Board, Anaerobe. (2004 - 2004)

     Member of the Editorial Board, Anaerobe. (2002 - 2002)

     Member of the Editorial Board, Applied Microbiology & Biotechnology. (2008 - 2008)

     Member of the Editorial Board, Applied Microbiology & Biotechnology. (2004 - 2004)

     Member of the Editorial Board, Applied Microbiology & Biotechnology. (2005 - 2005)

     Member of the Editorial Board, Electronic Journal of Biotechnology. (2008 - 2008)

     Member of the Editorial Board, Electronic Journal of Biotechnology. (2007 - 2007)

     Member of the Editorial Board, Applied Microbiology and Biotechnology. (2002 - 2002)

     Member of the Editorial Board, Electronic Journal of Biotechnology. (2006 - 2006)

     Member of the Editorial Board, Recent Patents on Biotechnology. (2008 - 2008)

     Member of the Editorial Board, Electronic Journal of Biotechnology. (2004 - 2004)

     Member of the Editorial Board, Electronic Journal of Biotechnology. (2005 - 2005)

     Member of the Editorial Board, Recent Patents on Biotechnology. (2007 - 2007)

     Member of the Editorial Board, Electronic Journal of Biotechnology. (2002 - 2002)

     Editor for Special Issue, Current Opinion in Biotechnology. (2008 - 2008)

    Supervised Theses & Dissertations
     Bethany K. Zolman, Ph.D. Genetic Analysis of Indole-3-Butyric Acid Response Mutants in Arabidopsis thaliana. (2002) (Thesis Committee Member)

     James Yager, Ph.D. Control of Perineurial Glial Growth in Drosophila melanogaster. (2002) (Thesis Committee Member)

     Heather Myler, Ph.D. Heparanase and Platelet Factor-4 Induce Smooth Muscle Cell Proliferation and Migration via Basic Fibroblast Growth Factor Release from the Extracellular Matrix: Implications in the Testenosis Process. (2002) (Thesis Committee Member)

     Monica Magidin, Ph.D. Genetic Analysis of Auxin Homeostasis: Conjugate Sensitivity and Auxin Supersensitivity. (2002) (Thesis Committee Member)

     Han Xu, Ph.D. Repressor Proteins: Ligand Binding, Thermodynamics and Assembly. (2002) (Thesis Committee Member)

     Ravi Vadali, Ph.D. Metabolic Engineering of Cofactors: Cofactor Engineering of CoA/acetyl-CoA to Increase Ester Productivity. (2003) (Thesis Committee Member)

     Wafa Elbjeirami, Ph.D. Genetic Modification of Smooth Muscle Cells to Enhance the Performance of Tissue Engineered Vascular Grafts. (2003) (Thesis Committee Member)

     Mary M. Watrous, M.S. --. (2003) (Thesis Committee Member)

     Emily Horton, Ph.D. Heterologous Expression of Alcohol Acetyltransferase Genes in Escherichia coli and Clostridium acetobutylicum for the Production of Esters. (2004) (Thesis Committee Member)

     Veronica Hall-O'Connell, Ph.D. Broad-Complex, a Mediator of Ecdysone Induced Stress Response in Drosophila melanogaster. (2004) (Thesis Committee Member)

     Miles Scotcher, Ph.D. Genetic Factors Affecting the Regulation of Solventogenesis in Clostridium acetobutylicum ATCC 824. (2004) (Thesis Director)

     A. Raquel Adham, Ph.D. Molecular and Genetic Analysis of IBA Homeostasis and Action in Arabidopsis. (2005) (Thesis Committee Member)

     Andrew Woodward, Ph.D. Genes, Organelles, and Molecules that Influence Plant Development Through Auxin Regulation. (2005) (Thesis Committee Member)

     David Ji, M.A. Elucidating the Mechanism of Action of Lmo4 in Vertebrate Eye Development. (2005) (Thesis Committee Member)

     Rafael Counago, Ph.D. Single Gene Evolution: A Punctuated History of Chance. (2005) (Thesis Committee Member)

     Song Liu, Ph.D. In Vitro Fusion Catalyzed by the Sporulation-Specific t-SNARE Light-chain Spo20p Is Stimulated by Phosphatidic Acid. (2007) (Thesis Committee Member)

     Sergio Iadevaia, Ph.D. Design of Artificial Genetic Networks to Regulate the Biosynthesis of Polyhydroxyalkanoate Copolymers with Desirable Structures. (2007) (Thesis Committee Member)

     Neda Nikravan, Ph.D. The Study of an Autocrine Signal Transduction Pathway that Represses Cell Proliferation in Dictyostelium discoideum. (2007) (Thesis Committee Member)

     Xiaoyang Bai, Ph.D. The Molecular Mechanism of Membrane Fusion in the Yeast S. cerevisiae. (2007) (Thesis Committee Member)

     Elizabeth Poggio, M.A. Molecular Genetic Analyses of Plant Peroxisome Functions. (2008) (Thesis Committee Member)

     Leighann Sullivan, Ph.D. Molecular and Genomic Analyses in Clostridium acetobutylicum. (2008) (Thesis Director)

     Andria Denmon, Ph.D. The Role of Base Modification on Tyrosyl-tRNA Structure, Stability, and Function in Bacillus subtilis and Bacillus anthracis. (2013) (Thesis Committee Member)

     Wanding Zhou, Ph.D. Graph-based Modeling and Evolutionary Analysis of Microbial Metabolism. (2013) (Thesis Committee Member)

     Jie Ma, Ph.D. Microbial processes influencing the attenuation and impacts of fuel ethanol releases. (2013) (Thesis Committee Member)

     Allan Henslee, Ph.D. Polymer-Based Approaches to Bone Tissue Repair and Regeneration. (2013) (Thesis Committee Member)

     Andrew Chang, Ph.D. T box mechanism and anticodon stem-loops: Molecular and structural studies of glycyl-tRNA anticodon stem-loops and their binding to T box specifier domain. (2013) (Thesis Committee Member)

     Mengyan Li, Ph.D. Genetic Catabolic Probes to Assess the Natural Attenuation of 1,4-Dioxane. (2013) (Thesis Committee Member)

     Hema Puppala, Ph.D. (Chemistry) Engineering silver nanoparticles: Towards a Tunable Antimicrobial. (2014) (Thesis Committee Member)

     Zachary Crannell, Ph.D. Development of Recombinase Polymerase Amplification (RPA) Assays to Diagnose Infectious Diseases (BIOE). (2015) (Thesis Committee Member)

     Dhiraj Paul, Ph.D. IIT Kharagpur, INDIA Examine. (2015) (Thesis Committee Member)

     Bahar Salimian Rizi, Ph.D. Nitric Oxide: The Missing Link in Omentum-Induced Metabolic Reprogramming of Ovarian Cancers (CHBE). (2015) (Thesis Committee Member)

     Premila Samuels Mohan Das, PhD Engineering recombinant myoglobins and hemoglobins for optimized globin stability. (Thesis Committee Member)

     Yun-Ting Kao, PhD Using chemical genetics and reverse genetics to study peroxisome biogenesis and peroxin functions. (Thesis Committee Member)

     Fenbeng Wang, PhD Structural characterization of enzymes involved in the biosynthesis of anti-tumor natural products. (Thesis Committee Member)

     Kathryn R Beaubout, PhD Evolutionary dynamics leading to tigecycline resistance in a hospital strain of Enterococcus faecalis. (Thesis Committee Member)

    Awards, Prizes, & Fellowships
     Fellow, American Institute for Medical and Biological Engineering

     Top 50 Reviewers Biotechnology & Bioengineering, (2013)

     American Chemical Society Section Award, Greater Houston Section of the ACS (2013)

     John S. Dunn Foundation Award, (2012)

     Fellow of the American Academy of Microbiology, (2006)

     E. Dell Butcher Professor in Biochemistry and Cell Biology, Rice University (2006)

     Hershel M. Rich Outstanding Invention Award, School of Engineering, Rice University (2005)

     Outstanding Associate, Lovett College (2004)

     Hershel Rich Invention Awardee, School of Engineering (2004)

     Member, Society for Industrial Microbiology

     Attendee, R.A. Welch Foundation Meeting, Houston, Texas (October 29-30, 2001)

     Member, American Chemical Society

     Member, American Society for Microbiology

     Member, American Association for the Advancement of Science

     Member, American Society for Biochemistry and Molecular Biology

     Member, American Society for Microbiology

     Member, American Association for the Advancement of Science

     Member, Society for Industrial Microbiology

     Attendee, American Society for Microbiology, 100th National Meeting, Los Angeles, California (May 21-25, 2000)

     Attendee, Robert A. Welch Foundation Meeting, Houston, Texas (October 23-24, 2000)

     Attendee, Clostridium 2000, The 6th International Workshop on the Regulation of Metabolism, Genetics and Development of the Solvent- and Acid-Forming Clostridia, Urbana, IL

     Member, American Society for Biochemistry and Molecular Biology

     Member, American Chemical Society

     Attendee, American Society for Microbiology, 99th National Meeting, Chicago, Illinois (May 30-June 3, 1999)

     Attendee, 7th Conference on Small Genomes, Washington, D.C. (November 13-17, 1999)

     Attendee, Robert A. Welch Foundation Meeting, Houston, Texas (October 25-26, 1999)